Effective component of Schisandra chinensis as well as preparation method and use thereof

An effective component, the technology of Schisandra chinensis, applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, can solve the problems of involving normal cells and side effects, etc.

Inactive Publication Date: 2009-05-20
TIANJIN TASLY PHARMA CO LTD
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective component of Schisandra chinensis as well as preparation method and use thereof
  • Effective component of Schisandra chinensis as well as preparation method and use thereof
  • Effective component of Schisandra chinensis as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1 Preparation of effective components of Schisandra chinensis

[0079] Take 250g of Schisandra chinensis, crush it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. Concentrate the extract into extract to obtain 8.6g, dissolve and load the sample with ethanol, and pass through the ODS-C18 column. First, use 1250ml of 30% ethanol as the mobile phase to obtain the eluate, discard it, and then replace it with 1250ml of 95% ethanol as the mobile phase. phase to obtain eluent II, and concentrate and dry the eluent II to obtain a sample; continue to separate the sample obtained by preparative liquid chromatography; separation conditions for preparative chromatography: the chromatographic column is a preparative column Zorbax SB-C18; 21.2mm×250mm , the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows:

[0080]

[0081] The flow rate is 10ml / min...

Embodiment 2

[0082] Example 2 Analysis of Schisandra Effective Components

[0083] The HPLC-ELSD joint analysis of the effective components of Schisandra chinensis of Example 1

[0084] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial ac...

Embodiment 3

[0087] Example 3 Schisandra active ingredient preparation

[0088] Take the active ingredient of Schisandra chinensis in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, move the material to drip irrigation, drop the medicine liquid into 6-8°C liquid paraffin, remove Oil, made 400 drop pills.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an effective composition of fructus schizandrae. The preparation process comprises the following: step one: a mixture of acetic ether and ethanol is used as a solvent and carries out extraction on the fructus schizandrae; step two: an extraction solution is subjected to column chromatography to obtain an eluant; and step three: the eluant prepared through liquid phase chromatographic gradient elution uses water and acetonitrile as a mobile phase; and the eluant is collected for 44.0 to 48.0 minutes to obtain the effective composition. The invention also provides a method for preparing the effective composition and application thereof. The effective composition is first obtained through the method and is first applied to the drug effect screening of various tumor cell strains; and as the effective composition is precise and has definite content, a convenient preparation process and good activity, the effective composition is suitable to be developed into a novel antitumor Chinese medicine.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from schisandra chinensis, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained fr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/57A61P35/00A61K131/00
Inventor 贺庆刘雳程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap