Effective component of cogongrass rhizome and preparation method thereof

An effective component, the technology of Imperatae imperatae, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-10-07
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of cogongrass rhizome and preparation method thereof
  • Effective component of cogongrass rhizome and preparation method thereof
  • Effective component of cogongrass rhizome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1 Preparation of Imperatae effective components

[0078]The preparation method of the effective components of Imperatae Rhizome of the present invention comprises the following steps: adding ethyl acetate and ethanol (1:1) after pulverizing the Rhizoma Imperatae medicinal material, heating and refluxing for 1 hour, extracting twice, and combining the filtrates to obtain an extract; Concentrate into an extract, dissolve and load the sample with ethanol, and pass through the ODS-C18 column. First, use 30% EtOH (5BV; 1BV≈250ml) as the mobile phase to obtain eluent I, and then replace it with 95% EtOH (5BV) as the mobile phase phase, to obtain eluent II, and concentrate and dry the eluent II to obtain a sample; continue to separate the sample obtained by preparative liquid chromatography; separation conditions for preparative chromatography: the chromatographic column is a preparative column ZorbaxSB-C18; 21.2mm×250mm, The mobile phase is water A and acetonitrile...

Embodiment 2

[0081] The analysis of the effective component of embodiment 2 coronal root

[0082] The HPLC-ELSD joint analysis to the effective component of the Rhizoma Imperatae of embodiment 1

[0083] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5...

Embodiment 3

[0086] Embodiment 3 Rhizoma Imperatae effective component preparation

[0087] Take the effective component C14 of Imperata coronata in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, after the material is changed, move it to the drip irrigation, and drop the drug solution into the liquid paraffin at 6-8°C , degreasing, made 400 dropping pills.

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PUM

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Abstract

The invention relates to an effective component of cogongrass rhizome as well as a preparation method and an application thereof. The preparation process of the effective component comprises the following steps: (1) a mixture of ethyl acetate and ethanol is used as a solvent to extract the cogongrass rhizome; (2) an extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (3) the eluent is eluted by prepared liquid chromatogram gradient and is collected for 56.0-60.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.

Description

Technical field: [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from the rhizome of Imperata coronata, a preparation, a preparation method and application thereof. Background technique: [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural c...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61P35/00
Inventor 贺庆刘雳程翼宇水文波窦静葛志伟
Owner TIANJIN TASLY PHARMA CO LTD
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