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Effective component of chebula fruit as well as preparation method and use thereof

A technology of effective components and myrobalan, which is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve the problems of normal cells and side effects.

Inactive Publication Date: 2009-05-20
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of chebula fruit as well as preparation method and use thereof
  • Effective component of chebula fruit as well as preparation method and use thereof
  • Effective component of chebula fruit as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1 Preparation of effective components of Myrobalan

[0078] 250 g of myrobalan was crushed, added with 8 times the amount of ethyl acetate: ethanol = 1:1, heated to reflux for 1 hour, extracted twice, added 8 times the amount of 70% ethanol to the drug residue, heated to reflux for 1 hour, extracted twice, and the filtrate was combined to obtain The extract was concentrated, dissolved in 5% ethanol and loaded, and passed through an ODS-C18 column. First, 5% ethanol (5BV; 1BV≈250ml) was used as the mobile phase, and then 50% ethanol (5BV) was used as the mobile phase. The eluent was separated by preparative liquid chromatography, and the separation conditions were as follows: the chromatographic column was an Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase was water A and acetonitrile B, and the gradient elution procedure was as follows

[0079]

[0080]

[0081] The flow rate is 10ml / min, and the column temperature is room temper...

Embodiment 2

[0082] Example 2 Analysis of the effective components of Myrobalan

[0083] HPLC-ELSD combined analysis of the effective components of Myrobalan in Example 1

[0084] Chromatographic conditions Column Agilent Zorbax SB-C18 column (4.6mm×150mm, 5μm); gradient elution is adopted, mobile phase A is 0.2% glacial acetic acid aqueous solution, and mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid in water, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile; at 10 minutes, mobile phase A is 70% 0.2% glacial acetic acid Aqueous solution and mobile phase B are 30% 0.2% glacial acetic acid in acetonitrile; at 30 minutes, mobile phase A is 50% 0.2% glacial acetic acid in water, and mobile phase B is 50% 0.2% glacial acetic acid in acetonitrile; 35 At 10 minutes, mobile phase A was 5% 0.2% glacial acetic acid in water and mobile phase B was ...

Embodiment 3

[0087] Example 3 Preparation of active ingredients of Myrobalan

[0088] Take the effective component of Myrobalan in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000, heat and melt, transfer the material to drip irrigation, drop the medicine into 6-8 ℃ of liquid paraffin, Oil was removed to obtain 400 dripping pills.

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Abstract

The invention relates to an effective component of myrobalam. The preparation process for the effective component comprises the following: step one, using a mixture of ethyl acetate and ethanol as solvent to extract the myrobalam; step two, using the ethanol to extract medicine dregs to obtain extract; step three, obtaining eluent through chromatography of the extract; and step four, using prepared liquid-phase chromatographic gradient to elute the eluent, and collecting the eluent for 48.0 to 52.0 minutes to obtain the effective component, wherein a mobile phase comprises water and methyl cyanide. The invention also discloses a preparation method for the effective component, a pharmaceutically acceptable preparation, and application thereof to preparing anti-tumor medicament.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from myrobalan, a preparation and a preparation method and application thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease endangering human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical drugs is not obvious, Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and traditional Chinese medicine also plays an important role in the adjuvant therapy of tumors. Paclitaxel is a typical natural compound with good anticancer activi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61K131/00
Inventor 贺庆刘雳程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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