Effective component of chebula fruit, preparation method and use thereof

An effective component, myrobalan technology, applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, which can solve the problems of side effects, involving normal cells, etc.

Inactive Publication Date: 2009-05-13
TIANJIN TASLY PHARMA CO LTD
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective component of chebula fruit, preparation method and use thereof
  • Effective component of chebula fruit, preparation method and use thereof
  • Effective component of chebula fruit, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The preparation of embodiment 1 Myrobalan effective component

[0069] 250g chebula is crushed, add 8 times the amount of ethyl acetate:ethanol=1:1, heat and reflux for 1 hour, extract 2 times, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, extract 2 times, and combine the filtrates to obtain Extract, after concentrating the extract, dissolve the sample with 5% methanol and pass it through the ODS-C18 column. First, use 5% ethanol (5BV; 1BV≈250ml) as the mobile phase, and then replace it with 50% ethanol (5BV) as the mobile phase Phase, continue to separate the eluent with preparative liquid chromatography, separation conditions: the chromatographic column is an Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows

[0070]

[0071] The flow rate is 10ml / min, and the column temperature is room temperature; the sample is dissolved in 10...

Embodiment 2

[0072] Embodiment 2 Analysis of the effective components of Myrobalan

[0073] The HPLC-ELSD joint analysis to the effective component of the chebula of embodiment 1

[0074] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial ...

Embodiment 3

[0077] Example 3 Preparation of effective components of Myrobalan

[0078] Take the active ingredient of myrobalan in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, and move the material to the drip irrigation after the material, and drop the medicine liquid into the liquid paraffin at 6-8°C, Degreasing, made 400 dropping pills.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an active component of a myrobalan fruit, and a preparation method and the application thereof. The preparation process comprises the following steps: firstly, a mixture of ethyl acetate and ethanol is taken as a solvent for extracting the myrobalan fruit; secondly, the eluent is obtained from an extracting solution through chromatographic column chromatography; and thirdly, the eluent is prepared by liquid chromatography gradient elution, the mobile phase includes water and acetonitrile, and the active component is obtained after the eluent is collected for 24.0-28.0min, 28.0-32.0min, 32.0-36.0min, 48.0-52.0min, 52.0-56.0min and 60.0-64.0min.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from chebula chebula, a preparation and a preparation method and application thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/185A61P35/00A61K131/00
Inventor 贺庆刘雳程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap