Active components of gallnut and preparation method and use thereof

An effective component, the gallnut technology, applied in the direction of drug combination, medical raw materials derived from arthropods, anti-tumor drugs, etc., can solve problems involving normal cells, side effects, etc.

Inactive Publication Date: 2009-08-19
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Active components of gallnut and preparation method and use thereof
  • Active components of gallnut and preparation method and use thereof
  • Active components of gallnut and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1 The preparation of Galla gall effective components

[0077] Take 250g of Galla gall medicinal material, crush it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into an extract, dissolved and loaded with ethanol, and passed through an ODS-C18 column. At first, 5% ethanol (5BV; 1BV≈250ml) is used as the mobile phase to obtain eluent I, and then 95% ethanol ( 5BV) as the mobile phase, to obtain eluent II, and to obtain a sample after concentrating and drying the eluent II; continue to separate the sample obtained by preparative liquid chromatography; separation conditions for preparative chromatography: the chromatographic column is a preparative column Zorbax SB-C18; 21.2mm×250mm, the mobile phase is water A and acetonitrile B, the gradient elution procedure is as follows:

[0078]

[0079] The flow rate is 10ml / min, and the column temperature is ...

Embodiment 2

[0080] Embodiment 2 The analysis of Galla gall effective components

[0081] The HPLC-ELSD joint analysis to the Galla gall effective component of embodiment 1

[0082] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic...

Embodiment 3

[0085] Example 3 Galla gall effective component preparation

[0086] Take the gallnut active ingredient of Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, move the material to the drip irrigation after the material, drop the medicine liquid into the liquid paraffin at 6-8°C, remove Oil, made 400 drop pills.

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PUM

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Abstract

The invention discloses an effective component for nutgall, a preparation method of the same and application of the same in resisting tumor. The effective component is obtained through the following steps that: the extraction is performed on the nutgall through a mixture of ethyl acetate and ethanol which is taken as a solvent, and an extracting solution is obtained; the extracting solution is subjected to chromatography in a chromatographic column to obtain an eluent; and the eluent is subjected to gradient elution by preparing liquid-phase chromatography, a mobile phase is water and acetonitrile, and the eluent is collected for 48.0 to 52.0 minutes or 52.0 to 56.0 minutes.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Galla galla, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from plants...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P35/00
Inventor 贺庆刘雳程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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