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Effective component of gamene and preparation and use thereof

An effective component, the technology of madder, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of gamene and preparation and use thereof
  • Effective component of gamene and preparation and use thereof
  • Effective component of gamene and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The preparation of embodiment 1 madder effective component

[0069] Crush madder, pass through a 20-mesh sieve after crushing, add 8 times the amount of ethyl acetate: ethanol = 1:1, heat and reflux for 1 hour, extract 2 times, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, and extract 2 times Once, the filtrate was combined to obtain the extract, after the extract was concentrated, the sample was dissolved with 5% ethanol, and the ODS-C18 column was crossed. At first, 1250ml of 5% ethanol was used as the mobile phase, and then 1250ml of 50% ethanol was replaced as the mobile phase to obtain a wash. Remove the liquid, and continue to separate the eluent by preparative liquid chromatography. Separation conditions: the chromatographic column is an Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure is as follows

[0070]

[0071] The flow rate is 10...

Embodiment 2

[0072] The analysis of embodiment 2 madder effective components

[0073] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic acid in water, and the mobile phase B was 95% 0.2% glacial acetic acid in acetonitrile. Soluti...

Embodiment 3

[0076] Embodiment 3 madder effective component preparation

[0077] Take the effective component of madder in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, after the material is changed, move it to the drip irrigation, and drop the drug solution into the liquid paraffin at 6-8°C, remove Oil, made 400 drop pills.

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Abstract

The invention relates to an effective component of madder root, a preparation method and the application thereof; the preparation process of the effective component of madder root in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting madder root; step 2, herb residue is extracted by ethanol to obtain extract; step 3, the extract is processed with chromatographic column chromatography to obtain eluate; step4, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 48.0-52.0 min is collected to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from madder, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from plants with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P35/00C07D307/94A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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