Effective component of cortex dictamni, preparation method and use thereof

An effective component, white fresh cortex technology, applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-05-13
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy,

Method used

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  • Effective component of cortex dictamni, preparation method and use thereof
  • Effective component of cortex dictamni, preparation method and use thereof
  • Effective component of cortex dictamni, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Example 1 The preparation of the effective components of white fresh cortex

[0151]Grind 250g of white fresh skin, add 8 times the amount of ethyl acetate:ethanol=1:1, heat and reflux for 1 hour, extract 2 times, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, extract 2 times, and combine the filtrates Obtain the extract, after concentrating the extract, dissolve the sample with 5% methanol, and pass through the ODS-C18 column. First, use 5% EtOH (5BV; 1BV≈250ml) as the mobile phase, and then replace it with 50% EtOH (5BV) as the mobile phase. Mobile phase, to obtain eluent, continue to separate eluent with preparative liquid chromatography, separation conditions: chromatographic column is Agilent preparative column (Zorbax SB-C18; 21.2mm × 250mm), mobile phase is water A and acetonitrile B, gradient The elution procedure is as follows

[0152]

[0153] The flow rate is 10ml / min, the column temperature is room temperature; the sample...

Embodiment 2

[0154] Example 2 Analysis of the effective components of Radix Dictatus Cortex

[0155] The HPLC-ELSD joint analysis of the effective components of the white fresh cortex of embodiment 1

[0156] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A...

Embodiment 3

[0159] Example 3 Preparation of Active Ingredients of Dictatorium Cortex

[0160] Take any one of the active components of the white fresh skin of Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, after the material is made, move it to the drip irrigation, and the medicine liquid is dropped to 6-8°C In liquid paraffin, degreasing, made 400 dropping pills.

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PUM

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Abstract

The invention relates to an active component of cortex dictamni radicis, and a preparation method and the application thereof. The preparation process comprises the following steps: step 1, the mixture of ethyl acetate and ethanol is taken as a solvent for extracting the cortex dictamni radicis; step 2, the eluent is obtained from an extracting solution through chromatographic column chromatography; and step 3, the eluent is prepared by liquid chromatography gradient elution, the mobile phase includes water and acetonitrile, and the active component is obtained after the eluent is collected for 24.0-28.0 min, 28.0-32.0 min, 32.0-36.0 min, 48.0-52.0 min, 52.0-56.0 min and 60.0-64.0 min.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Cortex Dictica, a preparation, a preparation method and application thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained fro...

Claims

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Application Information

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IPC IPC(8): A61K36/75A61P35/00A61K125/00
Inventor 贺庆刘雳程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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