Effective component of hedyotis diffusa and method of preparing the same and application

A technology of Hedyotis diffusa and active components, which is applied in medical preparations containing active ingredients, organic chemistry, drug combinations, etc., can solve problems such as side effects and normal cells involved, and achieve increased content and simple and clear chemical components , good pharmacological activity

Inactive Publication Date: 2008-02-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of hedyotis diffusa and method of preparing the same and application
  • Effective component of hedyotis diffusa and method of preparing the same and application
  • Effective component of hedyotis diffusa and method of preparing the same and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment one Hedyotis diffusa effective component

[0028] Crush 200g of Hedyotis diffusa medicinal material, add ethyl acetate and ethanol, heat and extract to obtain an extract, concentrate the extract into 21.6g of extract, mix it with silica gel, and separate it with a normal phase silica gel column , first use sherwood oil and ethyl acetate as mobile phase, obtain eluent I, discard it, then change chloroform and methanol as mobile phase, obtain eluent II, obtain sample 3.6g after eluent II is concentrated and dried; Continue to separate the obtained samples by preparative liquid chromatography; the separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, and gradient elution is performed. The sample was dissolved in 100% ethanol, separated by preparative liquid chromatography, and the solution was collected at a time period of 22.4 to 28.1 minutes, conce...

Embodiment 2

[0029] The preparation of embodiment two Hedyotis diffusa effective components

[0030] Grind 500g of Hedyotis diffusa medicinal material, add ethyl acetate and ethanol (1:0.8~1.2), heat and reflux for 0.8~1.2 hours, extract 1~3 times, combine the filtrates to obtain the extract, concentrate the extract into extract 41.8 g, and it is mixed with silica gel as a sample, and it is separated with a normal phase silica gel column. First, petroleum ether and ethyl acetate (47~53: 1) are used as mobile phase to obtain eluent I, which is discarded, and then Change chloroform and methanol (8~13:1) as mobile phase, obtain eluent II, obtain sample 7.0g after eluent II is concentrated and dried; Continue to separate the sample obtained by preparative liquid chromatography; Separation conditions of preparative chromatography : The chromatographic column is a preparative column, the mobile phase is water and acetonitrile, gradient elution, the flow rate is 9-11ml / min, and the column tempera...

Embodiment 3

[0031] The preparation of embodiment three Hedyotis diffusa effective components

[0032] Take 250 g of Hedyotis diffusa medicinal material, grind it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 23.2g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 3.9 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C 18; 21.2mm * 250mm), the mobile phase is water (A) and acetonitrile (B), and...

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Abstract

The invention provides the effective constituents of oldenlandia, which mainly contain two compounds of 2-methoxyl-3-hydroxyanthraquinone (A) and 2-methyl-3-hydroxyanthraquinone (B), wherein the content of A is 55-75%, the content of B is 15-25%. The constituents are obtained through steps of heating, extracting, concentrating, separating with silica gel column, eluting, concentrating and drying the eluent, continuously conducting liquid phase chromatographic separation, collecting the solution, concentrating and drying the solution. The effective constituents can be used for treating and preventing tumors.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extract, and in particular relates to an effective component extracted from Hedyotis diffusa, a preparation method thereof, and an application of the effective component in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61K45/06A61P35/00C07C50/34C07C46/10
Inventor 贺庆刘雳程翼宇瞿海斌水文波史强葛志伟窦静
Owner ZHEJIANG UNIV
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