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Salvia miltiorrhiza valid component, preparing method and application

An effective component, the technology of Salvia miltiorrhiza, applied in the field of preparation of medicines for treatment and prevention of tumors, can solve the problems of involving normal cells and side effects, and achieve the effects of improving content, easy quality control, and good pharmacological activity.

Inactive Publication Date: 2008-06-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Salvia miltiorrhiza valid component, preparing method and application
  • Salvia miltiorrhiza valid component, preparing method and application
  • Salvia miltiorrhiza valid component, preparing method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment one salvia miltiorrhiza effective components

[0026] Add ethyl acetate and ethanol after pulverizing 200g of salvia miltiorrhiza medicinal material, heat and extract to obtain the extract, concentrate the extract into 4.6g of medicinal extract, and mix it with silica gel as a sample, and separate it with a normal phase silica gel column. Petroleum ether and ethyl acetate are used as mobile phase to obtain eluent I, discard it, then change chloroform and methanol as mobile phase to obtain eluent II, and obtain sample 1.5g after eluent II is concentrated and dried; Phase chromatography continues to separate the obtained samples; separation conditions of preparative chromatography: the chromatographic column is a preparative column, the mobile phase is water and acetonitrile, and gradient elution is performed. The sample was dissolved in 100% ethanol, separated by preparative liquid chromatography, and the solution was collected at a time per...

Embodiment 2

[0027] The preparation of embodiment two salvia miltiorrhiza effective components

[0028] After pulverizing 500g of Salvia miltiorrhiza, add ethyl acetate and ethanol (1:0.8-1.2), heat and reflux for 0.8-1.2 hours, extract 1-3 times, combine the filtrates to obtain an extract, concentrate the extract into 9.0 g of extract, and Mix it with silica gel, and separate it with a normal phase silica gel column. First, use petroleum ether and ethyl acetate (47-53:1) as the mobile phase to obtain eluent I, discard it, and then replace it with chloroform and Methanol (8~13:1) was used as the mobile phase to obtain the eluent II, and the eluent II was concentrated and dried to obtain 3.1 g of the sample; the sample obtained by continuing to separate the preparative liquid chromatography; the separation condition of the preparative chromatography: chromatographic column To prepare the column, the mobile phase is water and acetonitrile, gradient elution, the flow rate is 9-11ml / min, and t...

Embodiment 3

[0029] The preparation of embodiment three salvia miltiorrhiza effective components

[0030] Take 250 g of Danshen medicinal material, pulverize it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 4.8g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 1.6 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C 18 ; 21.2mm * 250mm), mobile phase is water (A) and acetonitrile (B), and the g...

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Abstract

The invention provides effective components of radix salviae miltiorrhizae, mainly comprising TrijuganoneC as A compound, 7-Hydroxy-8, 13-abietadiene-11, 12-dione as B compound and Dihydrotanshinone I as C compound with respective content of 40 to 50 percent, 30 to 40 percent and 3 to 10 percent. The effective components can be obtained through following procedures: heating extraction, condensing, silica gel column separation, elution, condensing and drying of the eluted solution, liquid chromatography separation, solution collecting and condensing and drying of the solution. The effective components can be used in the preparation of drugs for treating and preventing tumors. With reasonable design, the invention can be quickly obtained and can be more favorable for quality control of the drugs.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extracts, and in particular relates to an effective component extracted from Salvia miltiorrhiza, a preparation method thereof, and an application of the effective component in preparing medicines for treating and preventing tumors. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61K31/343A61K45/06A61P35/00A61K125/00
Inventor 贺庆程翼宇瞿海斌刘雳水文波葛志伟窦静
Owner ZHEJIANG UNIV
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