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Effective component of morinda officinalis and preparation method thereof

An effective component, the technology of Morinda officinalis, applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-10-07
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of morinda officinalis and preparation method thereof
  • Effective component of morinda officinalis and preparation method thereof
  • Effective component of morinda officinalis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The preparation of embodiment 1 Morinda officinalis effective component

[0075] Morinda officinalis was crushed, sieved through a 20-mesh sieve, 8 times the amount of ethyl acetate:ethanol=1:1 was added, heated and refluxed for 1 hour, extracted twice, 8 times the amount of 70% ethanol was added to the dregs, heated and refluxed for 1 hour, Extract 2 times, combine the filtrates to obtain the extract, concentrate the extract, dissolve the sample with 5% ethanol, and pass it through the ODS-C18 column. First, use 1250ml 5% ethanol as the mobile phase, and then replace it with 1250ml 50% ethanol as the mobile phase , to obtain the eluent, and continue to separate the eluent by preparative liquid chromatography, separation conditions: the chromatographic column is an Agilent preparative column (ZorbaxSB-C18; 21.2mm×250mm), the mobile phase is water A and acetonitrile B, and the gradient elution procedure as follows

[0076]

[0077]The flow rate is 10ml / min, and the c...

Embodiment 2

[0078] The analysis of embodiment 2 Morinda officinalis effective components

[0079] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A was 5% 0.2% glacial acetic acid in water, and the mobile phase B was 95% 0.2% glacial acetic acid in acetonitri...

Embodiment 3

[0082] Embodiment 3 Morinda officinalis active ingredient preparation

[0083] Take the active ingredient 1 or 2 of Morinda officinalis in Example 1, mix 0.5g with 10.5g of polyethylene glycol-6000 evenly, heat and melt, and move the material to the drip irrigation after the material is formed, and the liquid drops to 6-8°C In liquid paraffin, degreasing, made 400 dropping pills.

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Abstract

The invention relates to an effective component of morinda officinalis as well as a preparation method and an application thereof. The preparation process of the effective component of the morinda officinalis comprises the following steps: (1)a mixture of ethyl acetate and ethanol is used as a solvent to extract the morinda officinalis; (2)dregs of a decoction are extracted with the ethanol so as to obtain an extracting solution; (3)the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; (4)the eluent is eluted by prepared liquid chromatogram gradient and collected for 28.0-32.0 minutes and 52.0-56.0 minutes to obtain the effective component by taking water and acetonitrile as flow phases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Morinda officinalis Radix Morinda officinalis, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical nat...

Claims

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Application Information

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IPC IPC(8): A61K36/746A61P35/00
Inventor 贺庆刘雳程翼宇水文波窦静葛志伟
Owner TIANJIN TASLY PHARMA CO LTD
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