Extract of an traditional Chinese medicine containing isopsoralen, preparation method and use thereof

A technology of isopsoralen and extract, which is applied in the field of traditional Chinese medicine extract, achieves the effect of simple preparation, obvious inhibitory effect, and quality control

Inactive Publication Date: 2007-08-01
ZHEJIANG UNIV
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, judging from the current research on psoralen, a higher concentration of psoralen is required to show anti-tumor effect when used alone

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extract of an traditional Chinese medicine containing isopsoralen, preparation method and use thereof
  • Extract of an traditional Chinese medicine containing isopsoralen, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 Preparation of psoraleae extract of the present invention

[0019] Weigh 500 g of psoraleae medicinal material, dissolve it in 2 L of pure water, heat and reflux for 5 hours, use a Soxhlet extraction device to collect volatile oil components, and obtain a volatile oil extract. Add 2L of industrial alcohol (95% ethanol) to the obtained dregs, extract in a water bath at 99° C. for 2 hours under reflux to obtain extract 1, and concentrate extract 1 to obtain about 100 g of extract. ODS-C18 column is used to separate it, and the sample extract is about 50g, and the flow rate is 10ml / min; first use 5% methanol as the mobile phase, then change 15% methanol as the mobile phase, then change 30% methanol as the mobile phase, and then Replace 50% methanol as the mobile phase, and then replace 70% methanol as the mobile phase to obtain eluent 1. The eluent 1 was concentrated and dried to obtain 1.55 g of the extract (psoralen extract).

Embodiment 2

[0020] Example 2 Composition analysis of psoraleae extract in the present invention

[0021] 1. Component analysis in psoraleae extract (high performance liquid chromatography)

[0022] Chromatographic conditions Chromatographic column: Agilent Zorbax SB-C18 chromatographic column (4.6mm×250mm, 5μm); column temperature: 30°C; mobile phase: (A) water, (B) acetonitrile, linear gradient elution, gradient elution program See Table 1; flow rate: 0.8mL min -1 ; Detection wavelength 190 ~ 400nm; analysis time 60min; injection volume: 10μL.

[0023] Table 1 Changes in mobile phase ratios for linear gradient elution

[0024] time

(min)

0

25

50

60

water%

Acetonitrile%

75

25

65

35

50

50

30

70

[0025] Preparation of the test solution Weigh this product, dissolve it in a volumetric flask with methanol, dilute to the mark, shake well, and you get it.

[0026] Determina...

Embodiment 3

[0031] Example 3 Determination of the content of active ingredients in the psoralen extract of the present invention

[0032] 1. Determination of psoralen, isopsoralen, neopsoralen isoflavones and psoralenin in psoralen extract (high performance liquid chromatography)

[0033] For chromatographic conditions, refer to the first step of Example 2, 1. Component Analysis in Psoraleae Extract (High Performance Liquid Chromatography). The recorded chromatographic time was 55 minutes.

[0034] Preparation of the test solution Weigh psoralen, isopsoralen, new psoralen isoflavones and psoralenin standard and psoralen extract samples, dissolve them in a volumetric flask with methanol, and dilute to Scale, shake well, that is.

[0035] 2. Determination method Precisely absorb the test solution, inject it into a high performance liquid chromatograph, measure the peak area absorbed at 300nm, and calculate the content of the sample by the external standard method.

[0036] Analysis Resul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention has provided Chinese traditional medicine extract which contains 10%-24% of angelicin, 8-19% part of psoralen, 4-12% of neobavaisoflavone and 2-9% of corylin. The preparing procedure of the invention includes the following steps: soaking the psoraleae medicine into pure water; heating and backstreaming to remove volatile oil; putting the gruffs into alcohol, heating and extracting the medicine; condensing the eluted solution into extractum; separating the extractum with ODS-C18 column using asgradient methanol from low density to high density; drying the eluted solution to get the extract which has definite structures and high content. The medical experiment shows that the extract can substantially inhibit sensitive tumor cell and multidrug resistance tumor cell so that it has obvious anti-tumor function. The invention has proper design and simple preparing method which can be used in preparing medicine that can inhibit sensitive tumor cell and multidrug resistance tumor cell.

Description

technical field [0001] The invention belongs to a traditional Chinese medicine extract, and relates to an effective Chinese medicine extract containing compounds such as isopsoralen, psoralen, new psoralen isoflavone and psoralenin extracted from psoralen medicinal material, and the extract The application of the drug in the preparation of drugs capable of inhibiting sensitive tumor cells and multi-drug resistant tumor cells. Background technique [0002] Cancer seriously threatens people's health, among which lung cancer, liver cancer, breast cancer, and leukemia are several cancers with high incidence rates. Lung cancer has become the largest cancer in my country, and its morbidity and mortality are increasing most rapidly. The incidence of liver cancer is second only to lung cancer, and the incidence of liver cancer in some areas is increasing year by year. About 260,000 patients die of liver cancer every year in the world, and nearly 100,000 in my country. Statistics s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/487A61K31/37A61P35/00
Inventor 程翼宇周晨光许冬梅黄欣
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap