Effective components of Cacumen Platycladi and preparation method thereof

An effective component, the technology of oriental cypress leaves, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-10-07
TIANJIN TASLY PHARMA CO LTD
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective components of Cacumen Platycladi and preparation method thereof
  • Effective components of Cacumen Platycladi and preparation method thereof
  • Effective components of Cacumen Platycladi and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1 Preparation of effective components of Platycladus orientalis leaves

[0106] The method for preparing the effective components of Platycladus orientalis leaves of the present invention includes the following steps: pulverizing the herbal medicines of Platycladus orientalis, adding ethyl acetate and ethanol (1:1), heating and refluxing for 1 hour, extracting twice, and combining the filtrate to obtain the extract; The extract was concentrated into an extract, dissolved in ethanol and loaded on the ODS-C18 column. First, 30% EtOH (5BV; 1BV≈250ml) was used as the mobile phase to obtain eluent I, and then changed to 95% EtOH (5BV) As the mobile phase, the eluent II is obtained, and the eluent II is concentrated and dried to obtain a sample; the sample obtained is continued to be separated by preparative liquid chromatography; the separation condition of the preparative chromatography: the chromatographic column is the preparative column ZorbaxSB-C18; 21.2mm× 250mm, t...

Embodiment 2

[0109] Example 2 Analysis of effective components of Platycladus orientalis leaves

[0110] HPLC-ELSD combined analysis of effective components of Platycladus orientalis leaves in Example 1

[0111] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm×150mm, 5μm); using gradient elution, mobile phase A phase is 0.2% glacial acetic acid aqueous solution, mobile phase B phase is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid in water, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid The aqueous solution and mobile phase B are 50% 0.2% glacial acetic acid in acetonitrile; at 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid in water, and mobile phase B is 95% 0.2% glacial acetic acid in acetonitrile; 35 At minutes, mobile phase A is 5% 0.2% glacial...

Embodiment 3

[0114] Example 3 Preparation of effective components of Platycladus orientalis leaves

[0115] Take the effective component 1 or 2 of Platycladus orientalis leaves of Example 1, mix 0.5g and 10.5g polyethylene glycol-6000 uniformly, heat and melt, transfer to drip irrigation after dissolving, and drop the medicine to 6-8℃ In the liquid paraffin, the oil was removed to prepare 400 drop pills.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to effective components of Cacumen Platycladi as well as a preparation method and a purpose thereof. The preparation process comprises the following steps: 1. the mixer of ethyl acetate and ethanol is used as a solvent for extracting Cacumen Platycladi; 2. eluent is obtained from an extracting solution through chromatographic column chromatography; 3. with the moving phase ofwater and acetonitrile, the eluent obtained by the phase color chromatographic gradient elution of a preparation solution is collected for 36.0-40.0 minutes, and the effective components are obtained from the eluent after 44.0-48.0 minutes.

Description

Technical field: [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from the leaves of Platycladus orientalis, a preparation and a preparation method and application thereof. Background technique: [0002] Tumor is a common and frequently-occurring disease, among which malignant tumors are currently the most serious type of diseases that endanger human health. At present, the treatment of malignant tumors in the industry is mainly surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genetic toxicity of botanical drugs is not obvious. Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation. Moreover, Chinese medicine also plays an important role in the adjuvant treatment of tumors. Paclitaxel is a typical natura...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C61/35C07C62/32C07C51/42C07D307/58A61K31/191A61K31/365A61K36/14A61P35/00
Inventor 贺庆刘雳程翼宇水文波窦静葛志伟
Owner TIANJIN TASLY PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap