Effective component of Atractylodes macrocephala and preparation and use thereof

An effective component, the technology of Atractylodes macrocephala, is applied in the field of Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemothera...

Method used

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  • Effective component of Atractylodes macrocephala and preparation and use thereof
  • Effective component of Atractylodes macrocephala and preparation and use thereof
  • Effective component of Atractylodes macrocephala and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0129] The preparation of embodiment 1 Atractylodes macrocephala effective component

[0130] Take 250 g of Atractylodes macrocephala, crush it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 18.2g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 2.9 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is an Agient preparative column (Zorbax SB-C18; 21.2mm×250mm), the mobile phase is water (A) and acetonitrile (B), and the gradi...

Embodiment 2

[0133] The analysis of the effective component of embodiment 2 Atractylodes Rhizome

[0134] The HPLC-ELSD joint analysis of the effective components of Atractylodes Rhizome of Example 1

[0135] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A ...

Embodiment 3

[0138] Example 3 Preparation of Active Components of Atractylodes Rhizome

[0139] Take 0.5 g of the active ingredient of Atractylodes Rhizoma Atractylodes Rhizome in Example 1 and mix evenly with 10.5 g of polyethylene glycol-6000, heat and melt, and then transfer the material to drip irrigation, drop the drug solution into liquid paraffin at 6-8°C, and remove oil , made 400 dropping pills.

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Abstract

The present invention relates to an effective component of Rhizoma Atractylodis and the preparation method and usage. For the effective component of Rhizoma Atractylodis of the present invention, the preparation process includes the following steps: step 1: the mixture of ethyl acetate and ethanol is used as a solvent to extract Rhizoma Atractylodis; step 2: the extract goes through column chromatography to obtain the eluent; step 3: the mobile phase of the eluent obtained from the preparation of liquid chromatogram gradient elution is water and acetonitrile, the eluent is collected for 28.1 to 33.9min so as to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Atractylodes macrocephala, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtain...

Claims

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Application Information

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IPC IPC(8): A61K36/284A61P35/00C07D307/92A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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