Fingerprint spectrum detection method for ethyl acetate part of ligusticum wallichii

A technology of ethyl acetate and detection method of Chuanxiong, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of restricting the clinical application of ethyl acetate of Chuanxiong, differences in active ingredients, unfavorable development of Chuanxiong, etc., and achieve a perfect quality evaluation system , Guaranteed clinical curative effect and simple method

Active Publication Date: 2020-03-24
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, due to the lack of quality detection methods for the ethyl acetate part of Chuanxiong, the active ingredients in the analgesic effective parts of Chuanxiong obtained from experiments and production are significantly different, resu...

Method used

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  • Fingerprint spectrum detection method for ethyl acetate part of ligusticum wallichii
  • Fingerprint spectrum detection method for ethyl acetate part of ligusticum wallichii
  • Fingerprint spectrum detection method for ethyl acetate part of ligusticum wallichii

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1 The fingerprint detection of the ethyl acetate part of Ligusticum chuanxiong of the present invention

[0027] 1) Preparation of reference substance solution: take chuanxionglide I, chuanxionglide H, chuanxionglide A, and ligustilide, accurately weigh them, add methanol to dissolve them, and make a reference substance stock solution with an appropriate mass concentration , and then accurately measure the above stock solution into the same volumetric flask, add methanol to dilute to the mark, and shake well to obtain the mass concentration of chuanxionglide I, chuanxionglide H, chuanxionglide A and ligustilide Mixed reference solution of 0.0210mg / mL, 0.0038mg / mL, 0.0197mg / mL, 0.0137mg / mL respectively;

[0028] 2) Preparation of the test solution: take 0.5 g of Rhizoma Chuanxiong sample powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered conical flask, add 50 mL of 70% ethanol to infiltrate it, weigh it, ultrasonically extract fo...

experiment example 1

[0036] 1 Materials and methods

[0037] 1.1 Drugs

[0038] Ligusticum chuanxiong, collected in the main production areas of Sichuan Province and introduced areas outside the province, was identified as Ligusticum Chuanxiong Hort. by Professor Ma Yuying of Chengdu University of Traditional Chinese Medicine. The sample information is shown in Table 1.

[0039] Table 1 Sample information of Rhizoma Chuanxiong

[0040]

[0041]

[0042] 1.2 Reagents

[0043] Ligustilide I (Lot No.: CHB170926), Ligustilide H (Lot No.: CHB161228), Ligustilide A (Lot No.: CHB160917), Ligustilide (Lot No.: CHB170224) were purchased from Chengdu Chromar Biotechnology Co., Ltd., the purity is ≥98%;

[0044] Methanol was chromatographically pure (Fisher Company, USA), water was pure water, and ethanol and ethyl acetate reagents were all chemically pure, provided by Chengdu Kelong Chemical Reagent Factory.

[0045] 1.3 Instruments

[0046] LC-2010A High Performance Liquid Chromatography (Shimad...

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Abstract

The invention discloses a fingerprint spectrum detection method for an ethyl acetate part of ligusticum wallichii. The fingerprint spectrum detection method adopts high performance liquid chromatography for detection. Through the detection method provided by the invention, the senkyunolide I, the senkyunolide H, the senkyunolide A and the ligustilide in the ligusticum wallichii can be measured atthe same time, the quality of effective parts with the analgesic effect can be controlled, the method is simple, convenient and reliable, a quality evaluation system of the ligusticum wallichii is perfected, the clinical curative effect of the ligusticum wallichii is guaranteed, and practical application and popularization value is achieved.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicines, in particular to a method for detecting fingerprints of ethyl acetate parts of Chuanxiong. Background technique [0002] Ligusticum chuanxiong is the dried rhizome of Ligusticum Chuanxiong Hort., an umbrella plant. It is a well-known authentic medicinal material produced in Sichuan. It has the effects of promoting blood circulation and qi, dispelling wind and relieving pain. Irregular menstruation, amenorrhea, dysmenorrhea, abdominal pain, headache, rheumatic arthralgia and other symptoms have been used by physicians of all dynasties as a good medicine for promoting blood circulation and relieving pain. Modern pharmacological studies have shown that Chuanxiong has pharmacological activities such as protecting cardiovascular and cerebrovascular, sedative and analgesic, anticoagulant, and is widely used clinically in the treatment of coronary heart disease, ang...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/36G01N30/34G01N30/74G01N30/8686G01N2030/027
Inventor 蒋桂华陈玲马俊邓薇连艳刘晓芬黄凤胡明勋袁茂华高必兴唐艺
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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