Establishment method of Dichondra repens fingerprint spectrum and Dichondra repens fingerprint spectrum

Abstract

The invention provides a dichondra fingerprint establishing method and a dichondra fingerprint, and belongs to the technical field of traditional Chinese medicine quality identification. Comprising the following steps: preparation of a reference substance solution: precisely weighing a proper amount of chlorogenic acid, umbelliferone, scopoletin and rutin reference substances, and adding methanol for dissolving to prepare the reference substance solution; preparing a test solution, namely precisely weighing dichondra repens medicinal material powder, precisely adding methanol for dissolving, weighing, carrying out ultrasonic extraction, cooling, re-weighing, complementing the lost weight with methanol, uniformly shaking, filtering with a microfiltration membrane, and taking a subsequent filtrate, thereby obtaining the test solution; hPLC (High Performance Liquid Chromatography) detection: chromatographic conditions are as follows: a mobile phase A is methanol and a mobile phase B is 0.1% phosphoric acid; the flow rate is 1.0 mL / min; the column temperature is 30 DEG C; the detection wavelength is 340 nm; the sample size is 10 microliters. According to the method, the defects of the existing quality control technology are overcome, and the quality control of the dichondra repens medicinal material is more perfect and scientific.

Description

technical field [0001] The invention relates to the technical field of quality identification of traditional Chinese medicines, in particular to a method for establishing a water chestnut fingerprint and the fingerprint. Background technique [0002] As the quintessence of our country that has been passed down for thousands of years, traditional Chinese medicine has remarkable curative effects. Fingerprint of traditional Chinese medicine is a quality evaluation model of traditional Chinese medicine that can be widely accepted at home and abroad. Japanese Kampo medicine used HPLC fingerprints to control the quality of traditional Chinese medicines and natural medicines in the 1980s. The European Community also applied fingerprints monitoring technology to the quality control of herbal medicines. The US Food and Drug Administration allows herbal health products to declare data Fingerprints are provided. In recent years, the research on fingerprints of traditional Chinese med...

AI Technical Summary

Problems solved by technology

At present, the quality control of people's horseshoe gold is only limited to the qualitative and quantitative analysis of a certain active ingredient or index ingredient among its many components, and there is a lack of standard control methods that fully reflect the quality of wolfberry.

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