Method for determining concentration of 5'-methoxyl-3',4'-methylenedioxyphenyl cinnamic acid isobutyl amide in plasma

A technology of isobutyl amide of cinnamic acid and methylene dioxyphenyl group, which is applied in the field of drug analysis, can solve the problem that the determination method is not reported in literature and the like, and achieves the effects of simple pretreatment method, simple operation and rapid method.

Inactive Publication Date: 2014-10-01
JIANGSU PROVINCIAL HOSPITAL OF TCM
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Problems solved by technology

[0003] At present, there is no literature report on the determination method of K

Method used

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  • Method for determining concentration of 5'-methoxyl-3',4'-methylenedioxyphenyl cinnamic acid isobutyl amide in plasma

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1: Determination of K6 concentration in human plasma.

[0024] 1. Experimental materials and instruments

[0025] K6 reference substance: provided by Suzhou Yihua Biomedical Technology Co., Ltd., batch number: 20131121; tinidazole reference substance: provided by the National Institute for the Control of Pharmaceutical and Biological Products, batch number: 100336-200402; test water: ultrapure water; methanol: chromatography Pure (Merck Company).

[0026] API4000LC / MS / MS combination instrument (Applied Biosystems, USA), Chromatography workstation: Analyst1.4.2; Mettler MT-5 electronic balance (Mettler, Switzerland); LEGEND MICRO17R refrigerated high-speed centrifuge (Thermo, USA) ; 80-2 centrifuge (Jiangsu Jintan Ronghua Instrument Manufacturing Co., Ltd.) Millipore Drict-Q5 ultrapure water machine (Millipore, France). Centrifugal concentrator: Labconco CentriVap Console (Labconco, USA)

[0027] 2. Liquid quality conditions

[0028] 1. Liquid chromatography conditions ...

Example Embodiment

[0048] Example 2: Determination of K6 concentration in human plasma

[0049] Referring to Example 1, 6 women took the extract of the effective part of the konjac leaf (containing K620.8mg) on ​​an empty stomach, and took it with 250mL of warm water; 4mL of peripheral venous blood was collected 3h after the administration, injected into the heparin tube, protected from light, and centrifuged (3000r·min -1 , 10min) prepare plasma. The treatment and determination conditions of the medicated plasma samples were the same. As a result, the K6 content in the plasma of the six women who were given the extract of the effective part of the leaf konjac was 9.323, 12.54, 12.55, 13.09, 5.011, 7.569 ng·mL, respectively -1 .

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Abstract

The invention discloses a met/hod for determining the concentration of 5'-methoxyl-3',4'-methylenedioxyphenyl cinnamic acid isobutyl amide in plasma. A liquid chromatography-mass system is adopted to determine. The method comprises the following steps: taking a to-be-determined sample first, adding a certain quantity of organic solvent for extraction, separating through a chromatographic column after pretreatment, and determining by using a mass spectrometry detector. The method disclosed by the invention has the advantages of rapidity, accuracy, high sensitivity, simplicity and convenience in operation and is suitable for determining the concentration of 5'-methoxyl-3',4'-methylenedioxyphenyl cinnamic acid isobutyl amide in plasma.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and relates to a method for analyzing and measuring drugs in vivo, in particular to a method for measuring the concentration of 5'-methoxy-3',4'-methylenedioxyphenylcinnamic acid isobutylamide in plasma. Background technique [0002] Piper Laetispicum (Piper Laetispicum C.DC.) is a plant belonging to the family Piperaceae. It is mainly produced in Hainan, Guangdong, Guangxi, Yunnan and other places. It is used for promoting blood circulation, reducing swelling and relieving pain. It is mainly used for bruises, blood stasis and swelling . Recent studies have found that betel has a strong antidepressant effect, and its activity is related to a series of amide compounds. Among them, 5'-methoxy-3',4'-methylenedioxyphenyl cinnamic acid isobutylamide (C15H19NO4) (K6) was the alkaloid with the highest content. [0003] At present, there is no literature report on the determination method of K6 i...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 张军储继红李长印居文政吴婷许美娟刘史佳
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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