Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry

A technology of carbamazepine and LC/MS, applied in the field of medicine, can solve the problems such as the need to improve the accuracy and sensitivity, the health hazards of operators, and the import cost of reagents, etc., and achieve high sensitivity, simple pretreatment method, and stable baseline. Effect

Inactive Publication Date: 2019-03-29
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The radioactive rays of the radioimmunoassay method have caused great harm to the health of the operators, and are rarely used in the world at present; the reagents required by the fluorescence polarization method are mainly imported and the cost is extremely expensive, and at the same time, expensive analytical instruments need to be purchased, and the above-mentioned The accuracy and sensitivity of the two monitoring methods need to be improved

Method used

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  • Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry
  • Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry
  • Method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Carbamazepine Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment

[0034] Carbamazepine (analyte): Toronto Research Chemicals or equivalent or higher grade standard

[0035] Oxybutynin (internal standard): Toronto Research Chemicals or equivalent or higher grade standard

[0036] The reagents used are shown in Table 1 below:

[0037] Table 1 Reagent Details

[0038] Reagent name

level

manufacturer

Acetonitrile

HPLC

J. T. Baker

formic acid

ACS

Adamas

[0039] Note: Reagents of the same grade or higher can also be used

[0040] The analytical equipment used is shown in Table 2 below:

[0041] Table 2 Details of equipment used

[0042] components

type

manufacturer

Binary pump (binary pump)

AC Pump

AB SCIEX

Degasser

Degasser

AB SCIEX

Column oven

AC Column oven

AB SCIEX

Autosa...

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Abstract

The invention discloses a method for determining concentration of carbamazepine in plasma by liquid chromatography mass spectrometry. A liquid chromatography mass spectrometry system is used for determining. A sample to be determined is added with a certain amount of mixed organic solvent to extract twice, is separated through a chromatographic column after pretreatment, and is detected by a massspectrometer. The method is rapid, accurate, high in sensitivity, and simple and convenient to operate. The method provides a basis for the blood drug concentration determination of carbamazepine. Thelinear range of a plasma standard curve in the method is 5 to 1000 ng/mL. The intra-batch and inter-batch precision RSD are less than +/-15%. The method is suitable for determining the concentrationof carbamazepine in the plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for determining the concentration of carbamazepine in blood plasma by liquid mass spectrometry. Background technique [0002] Carbamazepine (5H-dibenzo[b,f]azepine-5-carboxamide, 5H-dibenzo[b,f]azepine-5-carboxamide), its structural formula is as follows: [0003] [0004] Carbamazepine is a common psychiatric drug with antiepileptic, antitrigeminal neuralgia, antiglossopharyngeal neuralgia, prevention and treatment of manic depression and antiarrhythmia. However, due to the narrow range of its effective therapeutic concentration, the therapeutic effect cannot be achieved when the concentration is low, and side effects such as dizziness, lethargy, fatigue, vomiting, aplastic anemia, toxic hepatitis and shock can be caused after overdose. Therefore, it is very necessary to monitor drug concentration during treatment. [0005] At present, the methods for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 林明弘
Owner 徐州立顺康达医药科技有限公司
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