Method for detecting fingerprints of green bark medicinal material and its fingerprints

A technology of fingerprints and detection methods, which is applied to the establishment method of fingerprints and the field of fingerprints, which can solve the problems of mixed use, few chromatograms, and inability to establish characteristic fingerprints of green skin, so as to achieve complete retention, good reproducibility, and perfection The effect of the quality evaluation system

Active Publication Date: 2020-05-15
JIUZHITANG
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AI Technical Summary

Problems solved by technology

[0004] At present, in the existing research on the fingerprints of Qingpi, there are gas chromatography to establish fingerprints for volatile oil components, and there is no lack of liquid phase technology for quality control, but there are too few common peaks in the chromatograms, and the characteristic fingerprints of Qingpi cannot be established. Map, easy to mix with medicinal materials such as Citrus aurantium and Citrus aurantium

Method used

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  • Method for detecting fingerprints of green bark medicinal material and its fingerprints
  • Method for detecting fingerprints of green bark medicinal material and its fingerprints
  • Method for detecting fingerprints of green bark medicinal material and its fingerprints

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Experimental program
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Effect test

Embodiment 1

[0042] The establishment of embodiment 1 green bark medical material fingerprint

[0043] 1) Source of medicinal materials

[0044] In order to make the medicinal materials sufficiently representative, medicinal materials from different origins or different harvest seasons were collected as test samples. See Table 3.

[0045] Table 3 Sources of Qingpi Medicinal Materials

[0046]

[0047] 2) Instruments and reagents

[0048] Waters e2695-2998 high performance liquid chromatography, Empower chromatography workstation; column: ZORBAX SB C 18 Column (4.6mm×250mm, 5μm, American Agilent Company); KQ 3200 DE CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water system (Millipore Company, United States); AY220 electronic balance (Shimadzu Corporation, Japan) ).

[0049] 3) Selection of extraction method:

[0050] (1) Take green skin samples and crush them (pass through a No. 4 sieve), accurately weigh 1 g, accurately add 25 ml of 70% methanol, weigh, ultr...

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Abstract

The invention discloses a method for establishing a fingerprint of a green tangerine peel medicinal material and a fingerprint thereof, and relates to the technical field of fingerprints of Chinese medicinal materials. The method comprises the steps of (a), preparation of a reference solution; (b), preparation of a test solution; (c), HPLC detection conditions; (d), formulation of a standard fingerprint with hesperidin as a reference peak; (e), quality control of the fingerprint. According to the method, a pretreatment method for each test product is simple, characteristic components are completely reserved, the repeatability and stability are good, the degree of precision is high, and there is a certain specificity; the separation effect of each characteristic peak in the obtained fingerprint is good; the method can be used for identifying the authenticity and quality of the green tangerine peel medicinal material.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing fingerprints of green bark medicinal materials and the fingerprints thereof. Background technique [0002] The fingerprint of Chinese herbal medicine refers to the spectrum that can mark the characteristics of the Chinese herbal medicine, which is obtained by using certain analytical methods and testing instruments after proper treatment of the Chinese herbal medicine. One of the means has also been recognized by the international community. [0003] Qingpi is a commonly used traditional Chinese medicine, mainly the green peel of dried young fruit or immature fruit of Rutaceae plant orange (Citrus reticulata Blanco) and its cultivars. Qingpi is mainly divided into Sihuaqingpi and Geqingpi. From May to June every year, collect the young fruit that falls from the sun, and dry it in the sun, which is commonl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89G01N30/34G01N30/06G01N30/14
CPCG01N30/06G01N30/14G01N30/34G01N30/89G01N2030/146
Inventor 邓芸谷陟欣叶惠煊谭真鲁鲁袁莉黄胜朱丽
Owner JIUZHITANG
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