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Establishment method and fingerprints of the medicinal material of Prunus chinensis

A technology of fingerprints and establishment methods, which is applied in the establishment method of the fingerprints and fingerprints of the medicinal material of Shixiantao chinensis, and can solve the problems of inability to comprehensively and objectively detect and evaluate the quality of Shixiantao sibifolia, and achieve perfect quality evaluation System, ensure authenticity, and good stability

Active Publication Date: 2020-11-10
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in the prior art, most of the detections to the Xylophyllum can only roughly distinguish the authenticity of the Xylotype, and cannot comprehensively and objectively detect and evaluate the quality of the Xylotype

Method used

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  • Establishment method and fingerprints of the medicinal material of Prunus chinensis
  • Establishment method and fingerprints of the medicinal material of Prunus chinensis
  • Establishment method and fingerprints of the medicinal material of Prunus chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for establishing the fingerprint of the medicinal material Prunus chinensis, the specific experimental steps are as follows:

[0042] 1. Test materials

[0043] 1.1. Experimental materials

[0044] The 10 batches of Pholdota cantonensis medicinal material samples were mainly collected from different places in Guangxi, see (Table 1) for details, and were identified as Pholdota cantonensis Rolfe. .

[0045]

[0046] 1.2. Instrument

[0047]American Agilent 1260 high performance liquid chromatography (G1311C quaternary pump, G1329B autosampler, G1316A column oven and G1314B detector); KQ-500DA numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); BSA224S electronic analytical balance (Sartorius Scientific Instruments (Beijing) Co., Ltd.);

[0048] Electric constant temperature blast drying oven (Shanghai Yiheng Instrument Co., Ltd.); high-speed universal pulverizer (Hunan Xiangyi Experimental Instrument Development Co., Ltd.); HWS-...

Embodiment 2

[0118] A method for establishing the fingerprint of the medicinal material Prunus chinensis, comprising the following steps:

[0119] (1) Preparation of the reference solution: Accurately weigh the gallic acid standard, chlorogenic acid standard, and syringaldehyde standard, put them in 10mL volumetric flasks, dissolve them with methanol (analytical grade) and dilute to the mark, shake well , to prepare solutions with concentrations of 0.125g / ml, 0.0588g / ml, and 0.112g / ml respectively, and then filter through a 0.45μm microporous membrane to obtain the reference solution;

[0120] (2) Preparation of the test solution: Take 2.5 g of the fine powder of Shixian peach, accurately weighed, put it in a stoppered Erlenmeyer flask, add 80 mL of methanol with a volume concentration of 80% for 1.5 h, and use a volume concentration of 80% methanol solution to make up the weight, shake well and filter, take the continued filtrate, centrifuge for 10min, take the supernatant, filter it with...

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Abstract

The invention relates to the technical field of Chinese herbal fingerprints, particularly to a Pholidota cantonensis Rolfe Chinese herbal fingerprint establishing method and a fingerprint thereof. Themethod comprises the following steps of (1) preparation of reference solution, (2) preparation of sample solution, (3) high performance liquid chromatography and (4) measurement. The Pholidota cantonensis Rolfe Chinese herbal fingerprint establishing method and the fingerprint thereof have the advantages of good reproducibility, high precision and good stability; the established Pholidota cantonensis Rolfe Chinese herbal fingerprint can achieve relatively comprehensive control and assessment of the inherent quality of Pholidota cantonensis Rolfe, monitor the quality and identify Pholidota cantonensis Rolfe, ensure authenticity, safety, effectiveness, stability and consistency of herbs, provide a reference basis for further development and design of Chinese herb standards and regularize and ensure clinical medication.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing the fingerprints of the medicinal material Prunus chinensis and the fingerprints thereof. Background technique [0002] Although traditional Chinese medicine has a history of thousands of years of application and development, how to effectively evaluate the quality of Chinese medicinal materials has always been the focus and difficulty of research and application of Chinese medicinal materials. With the development of science and technology, fingerprint quality control technology has been proposed in the field of traditional Chinese medicine and has been widely recognized, and its application in the quality control of traditional Chinese medicine is also increasing; The atlas that can indicate the characteristics of the Chinese herbal medicine obtained by certain analytical means and testing instruments is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N30/8686
Inventor 梁爽朱华吴秀彩李鹏江媛萍
Owner GUANGXI UNIV OF CHINESE MEDICINE
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