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Method for constructing hplc fingerprint of fermented Cordyceps powder and preparation sterols and its fingerprint

A technology of fingerprinting and bacterial powder sterols, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of lack of quality evaluation system, difficulty in achieving effective control of the quality of fermented Cordyceps fungus powder and its preparations, etc.

Active Publication Date: 2020-10-09
JIANGXI GUOYAO PHARMA LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quality control of fermented Cordyceps powder and its preparations is mainly based on nucleoside chemical components, while sterols are rarely used as quality control targets, and there are few studies on the fingerprints of sterols in fermented Cordyceps powder. report, resulting in the lack of a perfect quality evaluation system, it is difficult to effectively control the quality of fermented Cordyceps powder and its preparations

Method used

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  • Method for constructing hplc fingerprint of fermented Cordyceps powder and preparation sterols and its fingerprint
  • Method for constructing hplc fingerprint of fermented Cordyceps powder and preparation sterols and its fingerprint
  • Method for constructing hplc fingerprint of fermented Cordyceps powder and preparation sterols and its fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1, the construction method of Jinshuibao tablet sterol HPLC fingerprint and its fingerprint

[0094] (1) Preparation of reference substance solution

[0095] Accurately weigh appropriate amounts of ergosterol, stigmasterol, β-sitosterol and carotene respectively, put them in the same 10mL volumetric flask, add methanol for ultrasonic dissolution, and set the volume to the mark to obtain the reference substance solution.

[0096] (2) Preparation of test solution

[0097] Weigh 1.5g each of 10 batches of Jinshuibao Tablets (batch numbers are 160401, 160501, 160502, 160601, 160701, 160702, 160703, 161202, 161203, 161204) and grind them into fine powder, add 10ml of methanol for ultrasonic extraction for 60min, take out and cool to room temperature Afterwards, centrifuge at 8000r / min for 10min, take the supernatant and pass through a 0.22μm microporous membrane, dilute the filtrate to 10ml with methanol, take an appropriate amount of the extract after distilling...

Embodiment 2

[0108] Construction method of HPLC fingerprint of sterols in Jinshuibao capsule and its fingerprint

[0109] (1) Preparation of reference substance solution

[0110] Accurately weigh appropriate amounts of ergosterol, stigmasterol, β-sitosterol and carotene respectively, put them in the same 10mL volumetric flask, add methanol for ultrasonic dissolution, and set the volume to the mark to obtain the reference substance solution.

[0111] (2) Preparation of test solution

[0112]Weigh 1.5 g of Jinshuibao Capsules (batch numbers 16010005, 16070345, 16080393, 16110701, 16090394, 16030133, 16050229, 16040185, 16020075, 16050347) 10 times, add 10 ml of methanol for ultrasonic extraction for 60 min, take out and cool to room temperature for 00 r / min For 10 minutes, take the supernatant and pass it through a 0.22 μm microporous filter membrane, dilute the filtrate to 10ml with methanol, take an appropriate amount of the extracted solution after constant volume, add potassium hydroxid...

Embodiment 3

[0123] The construction method of the fingerprint of the fermented Cordyceps fungus powder sterol of the present invention is optimized and finally determined through a large number of experiments, and the preferred experiments are as follows:

[0124] 1. Optimization of chromatographic conditions

[0125] (1) Selection of Elution System

[0126] Except for the conditions of the elution system, the basic steps for chromatographic detection of the test product are the same. In the experiment, methanol-water, acetonitrile-water, methanol-0.1% phosphoric acid water, acetonitrile-0.1% phosphoric acid water elution system were selected for research. The results show that the response of each component of methanol-0.1% phosphoric acid water elution system is small, and the resolution is poor, while the sterol components of acetonitrile-water and acetonitrile-0.1% phosphoric acid water elution systems do not have a peak within the corresponding time, so it is selected Methanol-wate...

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Abstract

The invention belongs to the field of drug analysis, relates a quality control method of fermented cordyceps taishanensis powder, and particularly relates to fermented cordyceps taishanensis powder, preparation sterols HPLC fingerprint spectrum and a building method thereof. The building method includes the steps of firstly, preparing a control solution; secondly, preparing a test solution; thirdly, using HPLC to determine the fingerprint spectrum, and performing similarity evaluation, to be more specific, separately precisely sucking the control solution and the test solution, performing HPLCseparation detection with the flow phase being a methanol-water elution system to obtain the sterol fingerprint spectrum of the fermented cordyceps taishanensis powder, and importing the fingerprintspectrum into similarity software to perform similarity evaluation.

Description

technical field [0001] The invention belongs to the technical field of drug analysis and relates to a quality control method for fermented cordyceps powder, in particular to a method for constructing a sterol HPLC fingerprint of a fermented cordyceps powder and its preparation and the fingerprint. Background technique [0002] Cordyceps sinensis is a dry complex of the subunit and larval corpse of the fungus Cordyceps sinensis of the family Ergotaceae that parasitizes on the larvae of the bat moth family insects. It is one of the precious Chinese medicinal materials in my country. Due to the over-exploitation of Cordyceps sinensis resources, coupled with the specificity of its host and the limitation of the environment, the resources of Cordyceps sinensis are becoming increasingly exhausted. Fermented Cordyceps powder is obtained by submerging the medicinal strains isolated from Cordyceps sinensis. Research has found that fermented Cordyceps powder has similar chemical compo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈丽华朱卫丰管咏梅杨明金晨赵加茜
Owner JIANGXI GUOYAO PHARMA LLC
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