Establishment method and fingerprint of the medicinal material of Lime citrus

A technology of fingerprints and establishment methods, which is applied to the establishment method of fingerprints of ponzu medicinal materials and the field of fingerprints, which can solve the problems of lack of quality evaluation system, weak control of the quality of ponzu medicinal materials, and difficulty in achieving effective quality control of ponchos, etc. , to achieve the effect of improving the quality evaluation system and monitoring quality

Inactive Publication Date: 2016-01-13
GUANGXI UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

But at present, there is no quality standard for the medicinal materials of shikanzi, and the quality control of the medicinal materials of shikanzi is very weak. It only includes routine inspections such as microscopic identification, lacks a perfect quality evaluation system, and it is difficult to achieve effective control of the quality of shikanzi.

Method used

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  • Establishment method and fingerprint of the medicinal material of Lime citrus
  • Establishment method and fingerprint of the medicinal material of Lime citrus
  • Establishment method and fingerprint of the medicinal material of Lime citrus

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Embodiment Construction

[0031] Below in conjunction with accompanying drawing example, the present invention is described in further detail:

[0032] This embodiment is a method for establishing the fingerprints of 10 batches of lime medicinal materials from different production areas and the fingerprints obtained accordingly:

[0033] The 10 batches of shikanzi medicinal materials collected from different production areas at different times include Guangxi Nanning, Guangxi Guilin, Guangxi Liuzhou, Guangxi Wuming, Guangxi Guangxi, Yunnan, Guangxi Jinxiu, Guangxi Yulin; the basic information such as the source of the test samples of each shikanzi medicinal materials As in Table 1:

[0034]

[0035] Instruments and medicines: UltiMate3000 high-performance liquid chromatography was used; there were 10 batches of lime medicinal materials, all of which were purchased from Chinese herbal pharmacies, and other reagents were chromatographically pure or analytically pure.

[0036] According to the followi...

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Abstract

The invention discloses a Chinese pothos herb fingerprint establishing method and a Chinese pothos herb fingerprint, and relates to the technical field of the fingerprints of traditional Chinese medicinal materials. The method comprises the following steps: 1, sample preparation: adding Chinese pothos herb powder into a test tube, adding a proper amount of a methanol solution, immersing for 24h, carrying out ultrasonic concussion for 40min, cooling, shaking up, carrying out coarse filtration, and filtering by using a microporous filtering membrane to obtain a sample solution; and 2, high performance liquid chromatography detection conditions: carrying out high performance liquid chromatography detection on the sample solution according to the following conditions: the chromatographic column uses octadecyl silane chemically bonded silica as a filler, the mobile phase is acetonitrile-0.085% phosphoric acid, the sample introduction amount is 10-25muL, a gradient elution mode is adopted, the detection wavelength is 310nm, the flow velocity is 0.8mL / min, and the column temperature is 20-35DEG C in order to obtain the Chinese pothos herb fingerprint. The Chinese pothos herb fingerprint established in the invention can be used for effectively monitoring the quality of Chinese pothos herb and identifying the truth of Chinese pothos herb.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing fingerprints of Chinese medicinal materials and fingerprints thereof. Background technique [0002] Although traditional Chinese medicine has a history of thousands of years of application and development, how to effectively evaluate the quality of Chinese medicinal materials has always been the focus and difficulty of research and application of Chinese medicinal materials. With the development of science and technology, fingerprint quality control technology has been proposed in the field of traditional Chinese medicine and has been widely recognized, and its application in the quality control of traditional Chinese medicine is also increasing; The atlas that can indicate the characteristics of the Chinese herbal medicine obtained by certain analytical means and testing instruments is one of the most effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 黄琼黄永春谢清若唐福宇刘新梅
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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