Chinese angelica medicinal material detection method

A detection method and technology for medicinal materials, which are applied in the field of quality testing of Angelica sinensis medicinal materials, can solve the problems of single testing index of Angelica Root Root, unfavorable quality control of Angelica medicinal materials, etc., achieve good accuracy and practical effect, improve safety, stability, and accuracy. high degree of effect

Inactive Publication Date: 2015-01-07
LONGXI ZHONGTIAN PHARM CO LTD
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  • Claims
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Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is that the detection index of Chinese angelica medicinal material is single in the prior art, is unfavorable for the quality control of Chinese angelica medicinal material, and then provides a kind of fast, comprehensive, highly targeted detection method of Chinese angelica medicinal material

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  • Chinese angelica medicinal material detection method
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  • Chinese angelica medicinal material detection method

Examples

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Embodiment 1

[0039] The content determination of ferulic acid in the angelica medicinal material of embodiment 1

[0040] See Table 1 for the source and place of origin of the tested angelica medicinal materials. They were numbered and detected according to different batches. The specific steps of the detection are as follows:

[0041] (1) Accurately weigh 10 mg of ferulic acid reference substance, put it in a brown measuring bottle, add 70% methanol to dissolve and dilute to 50 mL; accurately measure 3 mL of the solution, add 70% methanol to dilute to 50 mL, shake well to obtain The reference substance solution of 12 μ g ferulic acid;

[0042] (2) Accurately weigh 0.2g of Angelica sinensis medicinal material powder to be tested, add 20mL of 70% methanol, weigh it, heat and reflux for 30 minutes, let it cool and then weigh it, and use 70% methanol to make up for the lost weight, and take it after standing. The supernatant is filtered with a microporous membrane, and the subsequent filtrat...

Embodiment 2

[0048] The content determination of ligustilide in the angelica medicinal material of embodiment 2

[0049] 1 Instruments and reagents

[0050] 1.1 Instrument

[0051] Shimadzu LC-20AT high performance liquid chromatograph;

[0052] Shimadzu SPD-M10A VP diode array detector;

[0053] Mettler AL-204 electronic balance; SPSS11.5 software.

[0054] 1.2 Reagents

[0055] Ligustilide reference substance (Tianjin Mark Biotechnology Co., Ltd., content is 99.0%);

[0056] Methanol and acetonitrile are chromatographically pure, glacial acetic acid and phosphoric acid are analytically pure, and water is pure water.

[0057] 1.3 Medicinal materials tested

[0058] See Table 3 for the source and place of origin of the tested angelica medicinal materials, which are numbered and tested according to different batches below.

[0059] 2 Methods and Results

[0060] 2.1 Preparation of reference solution

[0061] Accurately weigh 10.14mg of ligustilide reference substance, place it in a...

Embodiment 3

[0087] Example 3 Detection of Pesticide Residues in Angelica Medicinal Materials

[0088] 1 Instruments and reagents

[0089] 1.1 Instrument

[0090] Clarus600 gas chromatography-mass spectrometry (US PE company), HS10260D ultrasonic extractor (Kunshan Ultrasonic Instrument Co., Ltd.), RE-52A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory).

[0091] 1.2 Reagent

[0092]Dichlorvos, methamidophos, acephate, tetrachloronitrobenzene, hexachlorobenzene, α-666, β-666, γ-666, δ-666, omethoate, dimethoate Azinon, pentachloronitrobenzene, monocrotophos, difenthion, phosphamide I, dimethoate, heptachlor, pentachloroaniline, chlorothalonil, chlorpyrifos-methyl, aldrin, captan, phosphamide II. Methyl parathion, pirimiphos-methyl, methyl pentachlorophenyl sulfide, metalaxyl, triadimefon, chlorpyrifos, malathion, fenitrothion, parathion, pendimethalin , cis epoxy heptachlor, trans epoxy heptachlor, triadimenol A, triadimenol B, trans chlordane, cis endosulfan, cis chl...

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Abstract

The invention belongs to the field of traditional Chinese medicine quality detection and relates to a Chinese angelica medicinal material detection method. The Chinese angelica medicinal material detection method comprises determination of ligustilide and ferulic acid content of a Chinese angelica medicinal material and determination of pesticide residue content of the Chinese angelica medicinal material. The Chinese angelica medicinal material detection method has high detection accuracy and simple and fast processes, can finish multiple detection indexes, has good accuracy and utility, is conducive to Chinese angelica medicinal material quality control and is conducive to improvement of drug use safety and stability.

Description

technical field [0001] The invention belongs to the field of quality detection of Chinese medicinal materials, and in particular relates to a quality detection method of angelica medicinal materials. Background technique [0002] Angelica is the dried root of the Umbelliferae plant Angelica sinensis. Angelica sinensis is sweet, pungent, and warm in nature. Angelica has a long history of medicinal use, and it is one of the commonly used Chinese herbal medicines in traditional Chinese medicine. It is known as "ten directions and nine returns". In the "Notice on Further Regulating the Management of Health Food Raw Materials" issued by the Ministry of Health, angelica is listed as a raw material that can be used in health food. Angelica is an authentic medicinal material in Gansu, Gansu Minxian Angelica (commonly known as "Mingui"), has a cultivation history of more than 1,700 years, and is the origin and main origin of Chinese Angelica. General Inspection Bureau (2002) No. 13...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 杨锡陈杰李波韩斌李登鹏宋平顺何禄仁赵建邦王兰霞杨静
Owner LONGXI ZHONGTIAN PHARM CO LTD
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