The invention discloses a preparation method and application of a fluorescent probe for detecting quintozene, and relates to the field of
quality monitoring of Chinese herbal medicines, in particular to a preparation method of a
triphenylamine fluorescent probe T and detection of quintozene. The defects that an existing quintozene detection method is time-consuming and high in equipment cost, needs professional operators and cannot be popularized can be overcome. The preparation method of the probe T comprises the following step: carrying out
coupling reaction on 4-(diphenylamino)
phenylboronic acid and 9-(2-
biphenyl)-10-bromoanthracene to prepare the fluorescent probe T. The fluorescent probe T prepared by the invention can realize
specific fluorescence'on-off 'detection of quintozene in a neutral environment, and the detection process is good in selectivity and high in sensitivity, and can
resist interference of various factors. The fluorescent probe T can be used for detecting quintozene in Chinese herbal
medicine American ginseng.