7 results about "Pentachloronitrobenzene" patented technology

The invention belongs to the technical field of biology, and particularly relates to a pentachloronitrobenzene rapid detection method based on enzyme catalysis reaction, which comprises the following steps: converting pentachloronitrobenzene into pentachlorophenol and nitrate radicals through an enzyme system consisting of iron thioflavin protein reductase, ferredoxin and nitrobenzene dioxygenase; and then nitrate radicals are specifically detected by a Griess reagent method so as to indirectly reflect quintozene. The detection method disclosed by the invention is good in specificity, short in detection time and remarkable in visual detection effect, and can be used for on-site rapid detection of quintozene.

The present application relates to wastewater treatment technical field, specifically to a kind of method for improving endocrine disruptor removal in wastewater by strengthening intermediate species adsorption, it includes the following steps: providing Ce-Co3S 4‑x Nano catalyst and peroxymonosulfate;Ce-Co3S 4‑x Nano catalyst and peroxymonosulfate are added to the wastewater containing endocrine disruptor to be treated, and Fenton-like catalytic reaction is carried out under stirring condition, and endocrine disruptor in wastewater to be treated is degraded.The method can efficiently and stably remove endocrine disruptor in wastewater, and can achieve almost 99.9% removal rate of bisphenol A within 9min, and 99.6% removal rate of pentachloronitrobenzene within 12min.

The invention discloses a preparation method and application of a fluorescent probe for detecting quintozene, and relates to the field of quality monitoring of Chinese herbal medicines, in particular to a preparation method of a triphenylamine fluorescent probe T and detection of quintozene. The defects that an existing quintozene detection method is time-consuming and high in equipment cost, needs professional operators and cannot be popularized can be overcome. The preparation method of the probe T comprises the following step: carrying out coupling reaction on 4-(diphenylamino) phenylboronic acid and 9-(2-biphenyl)-10-bromoanthracene to prepare the fluorescent probe T. The fluorescent probe T prepared by the invention can realize specific fluorescence'on-off 'detection of quintozene in a neutral environment, and the detection process is good in selectivity and high in sensitivity, and can resist interference of various factors. The fluorescent probe T can be used for detecting quintozene in Chinese herbal medicine American ginseng.

The invention discloses a method for degrading organochlorine pesticide residues by using endophytic bacteria separated from healthy ginseng. According to the method, an endophytic bacterium Pseudomonas poae nH121 obtained from healthy ginseng is utilized, and the strain can degrade quintozene and hexachlorobenzene after being fermented and can be used for removing quintozene and hexachlorobenzene compounds in an old ginseng field polluted by pesticides. The method is easy to operate, green, safe, efficient and economical, and a new method can be provided for removing organochlorine pesticide residues in the environment.

A method for preparing a protein that degrades pentachloronitrobenzene pesticide residues and a method for regulating trace elements are disclosed. The strain used in this method is *Bacillus polymyxa*, deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 7250. The method involves extracting *Bacillus polymyxa* protein with 40% saturated ammonium sulfate, which can be used to degrade pentachloronitrobenzene pesticide residues. The trace element Fe2+ significantly enhances the pentachloronitrobenzene degradation activity of *Bacillus polymyxa*, exhibiting strong adaptability to different terrains and the ability to adjust its working mode in real time according to environmental needs. The *Bacillus polymyxa* pesticide residue-degrading protein prepared by this invention can significantly degrade pentachloronitrobenzene pesticide residues in ginseng. The 40% saturated ammonium sulfate extraction of *Bacillus polymyxa* protein has the effect of degrading pentachloronitrobenzene, and the trace element Fe2+ significantly enhances its ability to degrade pentachloronitrobenzene. 2+ It significantly enhances the degradation of pentachloronitrobenzene by Bacillus polymyxa.

The present invention provides a microbial agent for degrading pesticide residues, a preparation method thereof and an application thereof, belonging to the technical field of microbiology. The microbial agent can simultaneously degrade various different pesticide residues and is composed of methylotrophic Bacillus sp. L7, Paenibacillus polymyxa DPM03 and an auxiliary agent. The microbial agent can simultaneously degrade one or more of fluazinam, BHC, quintozene, chlorpyrifos, DDT, atrazine and deltamethrin, overcoming the problem in the prior art that the microbial agent can only degrade one or a kind of pesticide residues with similar structures, and can achieve a greater degradation effect and a better soil remediation effect with a smaller application amount. Moreover, methylotrophic Bacillus sp. L7 and Paenibacillus polymyxa DPM03 can promote each other and have a synergistic effect, and the compound microbial agent of the two has a significantly better degradation effect on pesticide residues than the single use of methylotrophic Bacillus sp. L7 and Paenibacillus polymyxa DPM03.

The invention relates to a cultivation method for micro-spray irrigation, water, fertilizer and pesticide integrated regulation and control of panax notoginseng root rot in slope cropland, and belongs to the technical field of traditional Chinese medicinal material planting. Soil turning is conducted on the gentle slope land with the shady and cool back face where pseudo-ginseng is not planted in the earlier stage; disinfection, wherein pentachloronitrobenzene is added into the turned soil for disinfection, and then ditching, ridging and shed building are carried out; planting disease-free seedlings; the irrigation amount and the fertilization amount are adjusted in time according to the water requirement rule of each growth stage of the panax notoginseng, and the panax notoginseng is irrigated and fertilized in a micro-spray irrigation mode; field management and pesticide spraying: spraying an acetaldehyde solution in a flowering stage and a fruiting stage for 1-3 times according to requirements per week, and controlling panax notoginseng root rot for cultivation. According to the method, through scientific management measures, disinfection and efficient pesticide, the morbidity rate of common panax notoginseng root rot is reduced to 4.5% from 20%, and the morbidity rate of panax notoginseng root rot is effectively reduced.