217 results about "Organochlorine pesticide" patented technology

The invention discloses a method which can carry out qualitative screening to 242 compounds (drugs or toxicants) simultaneously. A mode of liquid chromatogram-mass spectrometry (LC-MS / MS) multi-reaction monitoring (MRM) is adopted to carry out determinedness to objects by two pairs of parent ion-daughter ion pair and retention time. The 242 drugs or toxicants comprise toxic products such as opioids, amphetamine and cocaines and the like, bromazepam such as benzodiazepines and barbiturates, and common drugs, alkaloid, pesticide (including organophosphates, carbamates, pyrethroid pesticide residues and organochlorine pesticide), weed killer, raticide and the like.

The invention relates to the speeding up degradation of organic pollutant in the soil, which uses strong reduction feature of metal nulvalent, synthetic metal oxidation and hydro oxidation, effectively reducing organochlorine insecticide and other contaminating pollutant, improving the biological degradation feature of organic pollutant. The above pollutant can be further degraded with the participation of organic acid. It can effectively realize the original position restoring of contaminated soil, particularly for the control and restoring of heavy polluted soil.

The invention discloses a sample pretreatment method for detection of organochlorine pesticide residue in food and is characterized by comprising the following steps: water is added to a food sample, sample solution is swirled, and then inorganic salt and hydrophilic organic solvent are added to the sample solution, the sample solution is swirled and shaken uniformly, the sample solution undergoes phase splitting after centrifugation at room temperature, the upper-phase liquid is purified through dispersion solid-phase extraction method, the extraction temperature is 20-30 DEG C, and finally the upper-phase liquid is detected by gas chromatography; and the mass percent of the inorganic salt accounts for 5%-35% of the total mass of the system, the mass percent of the hydrophilic organic solvent accounts for 10%-50% of the total mass of the system, and water is of the residual amount, and the total mass of the system is the mass sum of water contained in the sample, the added water, the inorganic salt and the hydrophilic organic solvent. According to the sample pretreatment method, a dual-water-phase system consisting of the hydrophilic organic solvent, the organic salt and water is utilized, the partition coefficient is increased by means of changing the types and concentrations of the organic solvent and the inorganic salt, and the organochlorine pesticide residue in the sample can be directly extracted at room temperature.

The invention discloses multielement ex-situ remediation equipment and method used for organo-chlorine pesticide contaminated soil, belongs to the technical field of materializing remediation equipment for contaminated sites. The equipment comprises a stirring device, an eluent injecting device, an eluent recovery device, an ultrasonic generator and a heater. The equipment has extremely strong remediation broad-spectrum performance for organo-chlorine pesticide site soil with different characteristics such as organo-chlorine pesticide substance types, soil contamination incidents, soil contamination concentration, soil organic matter content, soil texture and the like, and a removal rate by single or repeated continuous elution is higher (larger than or equal to 85%); the equipment can form a good matching technology with a patent eluent (ZL201010547630.2), and is developed specially aiming at solving actual contamination situation of organo-chlorine pesticide sites in our country; and during a process of manufacturing the equipment, on the basis of synergistic effects generated by multielement strengthening components such as stirring, ultrasonic, heating and the like, the parameter condition design is optimized, the distribution is scientific and reasonable, and the optimal integration of multielement strengthening components is reflected.

The invention discloses a method for preparing modified activated charcoal. The method comprises the following process steps that: a woody material is dried, thermally decomposed and carbonized at a high temperature; charcoal blocks after carbonization are crushed, ground and sieved so as to obtain charcoal powder; the charcoal powder which is ground and sieved is adequately soaked in a hydrochloric acid solution and a sodium hydroxide solution in turn; the charcoal powder which is soaked in the hydrochloric acid solution and the sodium hydroxide solution and dried is activated in a high-temperature furnace; and the activated charcoal powder after high-temperature activation and anhydrous sodium sulfate are put into a glass chromatography column containing acetone in turn so as to prepare an activated charcoal glass chromatography column. The method for preparing the modified activated charcoal has the advantages of low cost, easy operation and applicability to the separation of sixteen types of organic chloride pesticides and ten types of polychlorinated biphenyls in marine environmental samples (marine organisms, marine sediments and seawater).

The invention relates to a method for remediation of contaminated soil, in particular to a method for combined remediation of DDTs-PAHs combined contaminated soil. Biosurfactant rhamnolipid is combined with efficient mixed degrading bacteria to strengthen and promote organic chloride pesticide DDTs and PAHs left in facility agriculture soil for decomposition and conversion; and meanwhile low-accumulation vegetable crops are planted, and microbial growth and degradation of the organic contaminant DDTs and PAHs are further strengthened through the plant root system metabolism and the chemical stimulation function of root exudates. The method has the beneficial effects that damage to the environment is small, no secondary contamination is generated, cost is low, large-scale operation is easy, and the production and economic income are not influenced; the method is a safe and effective in-situ remediation technology capable of achieving remediation and production at the same time; and the method is suitable for remediation of the intermediate and low contamination facility agriculture soil where crops are still planted.

The invention discloses a method for treating persistent organic pollutant through base catalytic decomposition with the help of microwaves, which is to use base catalytic decomposition technology with the help of the microwaves to treat various wastes containing the persistent organic pollutant (POPs), utilize interaction of the microwaves and microwave absorbing materials as a catalyst to quickly raise the reaction temperature, and complete quick dechlorination of POP substances in the waste to be treated in the presence of base and a hydrogen donor together. The method can treat waste organochlorine pesticide (OCPs), polychlorinated biphenyl (PCBs) oil in transformers, soil and deposit severely polluted by the OCPs and the PCBs, waste incineration fly ash containing high-concentration bioxin (PCDD / Fs) and salt slurry in the chlorine alkali industry and the like, has short treatment time and high dechlorination efficiency, and can realize quick treatment of the various wastes containing the POPs.

The invention provides a method for detecting an organochlorine pesticide residue in soil, which adopts a blank matrix without pesticides to match a standard solution for calibration, thus prevents a measured value from being much higher than an actual value for some organochlorine pesticides due to matrix effect, and improves the detection precision; The detection method of the invention adopts Florisil and anhydrous sodium sulfate for the purification of soil extract, and is simple in operation and good in purification effect; in addition, the invention optimizes gas chromatography conditions, can detect residues of as many as 21 organochlorine pesticides simultaneously, and accords with current development trend for organochlorine pesticide residue detection in soil.

The invention provides a peony and liquorice soup formula granule, and a preparation method and a detection method of the peony and liquorice soup formula granule. The preparation method comprises the steps of weighing peony and liquorice at a mass ratio of 1:1, adding water for decocting and extracting, merging filtrate, decompressing and condensing the filtrate to obtain a concentrated solution, drying the concentrated spray, adding water for granulating and sieving, and obtaining the peony and liquorice soup formula granule. The detection method comprises the steps of identifying the peony and liquorice soup formula granule by a thin-layer chromatography, and measuring the heavy metal content, the organo-chlorine pesticide residual amount, the fingerprint spectrum, the extract content and the paeoniflorin content of the peony and liquorice soup formula granule. The peony and liquorice soup formula granule inherits advantages of a traditional Chinese medicine single-formula granule, fully considers interaction during decocting (or other processing) of a decoction piece, and has a higher value, and the detection method allows the quality of the peony and liquorice soup formula granule to be controlled more effectively, so that the peony and liquorice soup formula granule is safer to use.

The invention discloses a method for determining organochlorine pesticide residues in tobaccos or tobacco products. The method comprises the following steps: leaching a wetted tobacco leaf sample with an organic solvent; adding a drying agent to dry extract liquid, and then purifying the extract liquid with a magnetic adsorbent; and finally detecting by an on-line gel permeation chromatography-gas chromatography-tandem mass spectrometry instrument, and building a method for determining organochlorine pesticide residues in tobaccos or tobacco products. According to the method disclosed by the invention, the defects of an existing determination method are overcome; a sample pretreatment method and an instrument detection condition are optimized; compared with the prior art, the method has the advantages that the testing cost is reduced, and has the advantages of simplicity in operation, high purifying capacity, high sensitivity and the like.

The invention discloses a method for treating organochlorine-pesticide-contaminated soil through activated persulfate oxidization, and belongs to the field of soil remediation. The method is characterized in that ferrous iron, hydrogen peroxide and activated persulfate are utilized to generate strong-oxidizability sulfate radical anion and hydroxyl free radical, thus realizing the oxidization and degradation of organochlorine-pesticide in soil. Compared with an existing Fenton method and a ferrous individually-activated persulfate method, the method has the advantages of being high in activating efficiency, and high in contaminant degradation efficiency; in addition, the method is simple to operate, wide in pH applying scope, obvious in oxidization and degradation effect, and high in potential in the field of environment and soil contamination control and treatment.

The invention relates to a magnetic remediation material for organic matter pollution in soil or water, and preparation as well as application thereof. Preparation comprises the following steps of (1) preprocessing clay mineral, including drying, smashing and sieving; (2) adding the sieved clay mineral into a molysite solution, and adding other metal salts, heating up the mixed turbid liquid to 30-90 DEG C and continuously stirring for 0.5-5 hours to make the liquid steady, adding alkali liquor to increase the pH value of the solution to 8-11, and stirring continuously for 0.5-2 hours; (3) separating, drying and smashing solid sediment, and executing thermal treatment, thereby obtaining the magnetic remediation material capable of magnetically separating the organic matter pollution in soil or water. As compared with the prior art, the remediation material provided by the invention has magnetic separability, can be recycled for cycle use, also has relatively high stability and catalytic activity, as well as good ecological compatibility, and is suitable for in-situ or ex-situ remediation of soil or water polluted by polycyclic aromatic hydrocarbon, polychlorinated biphenyl, Dioxin, organo-chlorine pesticides, and the like, and degradation intermediate products thereof.

The invention discloses a method for remedying the soil contaminated by DDT (1,1,1-trichloro-2,2-bis-(pchlorophenyl)ethane) as a pesticide residue through earthworm-based bioaugmentation and biodegradation. The method comprises the following steps: feeding eiseniafoetida or amynthas rubusto in the contaminated soil; further feeding fermented chicken manure and / or cattle manure and fully and evenly mixing the chicken manure and / or cattle manure and the soil; and feeding the fermented chicken manure and / or cattle manure every three months, wherein the feeding density of the eiseniafoetida or amynthas robust is 5 to 30 earthworms per each kilogram of soil; the mass ratio between the chicken manure and / or cattle manure and the soil is 0.2-1:1; and the water content of the soil in the soil remediation is maintained at 55% to 65%. The invention has the advantage that the requirements for the external environment are low, so the invention is widely applicable to most of the soil contaminated by the DDT at current; and the method for remedying the soil contaminated by DDT as an organic chlorine pesticide is simple, effective and low-cost.

The invention discloses a treatment method of organochlorine pesticide polluted soil, which comprises the following steps: (1) pretreatment: detecting and analyzing organochlorine pesticide polluted soil; digging out the organochlorine pesticide polluted soil, and mechanically and manually screening to remove impurities; adding quicklime, stirring, piling, and carrying out thermal desorption to remove volatile organic pollutants, thereby obtaining pretreated organochlorine pesticide polluted soil; and (2) cement rotary kiln treatment: after metering the pretreated organochlorine pesticide polluted soil, grinding with clay, iron powder and limestone to obtain cement raw meal, homogenizing the cement raw meal, sending into a preheater to carry out preheating, sending into a decomposition furnace to carry out decomposition, sending into a cement rotary kiln, calcining to obtain cement clinker, cooling the cement clinker, and grinding to obtain cement. The treatment method disclosed by the invention has the advantages of no toxicity, low cost and no secondary pollution on the environment, can change wastes into valuable substances, and effectively utilizes the resources.

The invention discloses a method for remedying organo-chlorine pesticide (OCPs) contaminated soil by combining a zero-valent iron composite material with microorganisms. The method comprises the following steps that 1, a biomass material is taken, and biochar is prepared by means of the biomass material and serves as a nanometer zero-valent iron loaded material; by means of a liquid phase reduction method, a biochar loaded nanometer zero-valent iron material (BC / nZVI) is prepared; 2, specific degrading bacteria are screened and separated; 3, to-be-remedied soil is pretreated; 4, the to-be-remedied soil is treated. Accordingly, aiming at the damage of ZVI to the soil structure and soil environment and the defect of the microorganisms to OCPs degrading capability, the novel BC / ZVI compositematerial is combined with the microorganisms for remedying, the ZVI reductive dechlorination effect, the biochar adsorption and soil improvement effect and the microorganism degradation effect are fully utilized, and remedying on OCPs contaminated soil is achieved.

The present invention provides a method for examining residual organochlorine pesticides in Chinese traditional medicine, including steps of: (1) preparing a certain concentration standard solution containing nine organochlorine pesticides; (2) examining standard solution under the propriety chromatogram conditions to obtain standard chromatogram graph; (3) preparing specimen: dipping specimen powder in acetone, ultrasonic extracting by petroleum ether, purifying with sulfuric acid, decompressing and concentrating; (4) examining specimen under the same chromatogram conditions of reference substance to obtain chromatogram graph of specimen; (5) comparing the chromatogram graph between specimen and reference substance by preservation time contrast method to determine corresponding characteristic peak to calculate content by external standard method. The present method has the merits of simple operation, less disturb to tested specimen, stable data result, no damage to examining device, high recovery rate and precision, and good system reproducibility and applicability.

The invention relates to a method capable of simultaneously measuring organochlorine pesticide concentration and synthetic musk concentration in human serum. According to the method, the aim of measuring organochlorine pesticide and synthetic musk in the serum is realized through a chemical pretreatment method of liquid-liquid extraction, gel permeation chromatography and silica gel alumina column, in combination with a detection technology of gas chromatography-electron capture detection (GC-ECD) and gas chromatography-mass spectrometry (GC-MS). The method provided by the invention belongs to the technical field of physico-chemical analysis measurement of organic chemicals. In the method provided by the invention, the serum pretreatment process comprises organic solvent liquid-liquid extraction, gel permeation chromatographic column degreasing, silica gel alumina column purification, separation and concentrating to constant volume, finally, GC-ECD and GC-MS are used for detection, detection spectrograms of organochlorine pesticide and synthetic musk in serum sample are respectively obtained, and concentration values of organochlorine pesticide and synthetic musk in the serum are calculated through a special formula. The method provided by the invention is a steady, relatively high-sensitivity and high-precision effective measurement method.

The invention relates to a method for preparing a Y2O3:Tb<3+>@SiO2-NH2 fluorescence sensor array used for detecting an organic chlorine pesticide. The marking recognition sites in the fluorescence sensor array are mutually effected with organic chlorine pesticide molecules (target molecules), the target molecules and Tb<3+> on the recognition sites form an organic ligand through coordination, and change of the fluorescence intensity of Tb<3+> is used for detecting the target molecules. The method comprises the following three steps: preparing Y2O3:Tb<3+> fluorescent powder, then synthesizing a Y2O3:Tb<3+>@SiO2-NH2 fluorescence probe having capability for identifying the marking target molecules, finally, through a micromachining technology and a plasma etching method, dropping the fluid suspension of the fluorescence probe having the capability for identifying the marking target molecules on a silicon chip, naturally drying the silicon chip, and spontaneously putting the fluorescence probe materials in a micro hole to obtain the fluorescence sensor array for high-selectivity identification and high sensitive signal detection on the target molecules.

The invention relates to a DDT (dichlorodiphenyltrichloroethane)-degrading pseudomonad strain, and specifically provides a pseudomonad strain capable of degrading DDT, which can be used for the biological intensified treatment of DDT pollutants and can help to degrade DDT organochlorine pesticides. The DDT-degrading pseudomonad strain is named pseudomonad No.12-3; the strain was preserved by China General Microbiological Culture Collection Center on November 8th, 2011; and the preservation number is CGMCC NO.5449. The pseudomonad No.12-3 has the characteristic of degrading DDT organochlorine pesticides; the DDT degradation rate can be up to 51.6%; and secondary pollution can not be caused to the environment. Thus, the DDT-degrading pseudomonad strain can help to solve the problems of DDT degradation, pollution remediation and the like, thereby having high application value.

The invention discloses a method for remedying soil polluted by organo-chlorine pesticides (OCPs) on the basis of the solubilization, elution and microorganism strengthening effects of a surface active agent. The method includes the steps that firstly, soil is cleaned once on the basis of the solubilization and elution effects of the surface active agent so that over 85% of OCPs in the soil can be removed; the cleaned soil is directly stacked at the ventilated location of a work shed, the residual surface active agent is used for improving the community structure and activity of indigenous microorganisms and strengthening the indigenous microorganisms to degrade residual OCPs in the soil, and finally the soil meets the environmental safety standard. The method is easy to implement, economical, efficient, environmentally friendly and safe, and can be applied to remediation of OCPs organic polluted sites / soil on a large scale.

The invention provides a method for photocataytic degrading organ-chlorine pesticide in contaminant with nanometer TiO2. The method comprises following steps: drying the standard pesticides prepared with acetone by nitrogen; adding into nanometer TiO2 powder and water, getting mixed suspending liquid of TiO2 and pesticides; filling into air into the mixed liquid after ultrasonic vibration; irradiating directly with ultraviolet lamp for disinfection, removing TiO2 powder with filter after photocataytic degradation; extracting the liquid with skellysolve B, and getting the organic phase. The organic phase is the target object. The method is characterized by low cost, no pollution, and simple degradation process with short time. The organ-chlorine pesticide can be degraded in 30 minutes. The method can be used to treat organ-chlorine pesticide in wastewater or other.

The present invention provides a method for examining residual organophosphorus pesticides in Chinese traditional medicine, including steps of: (1) preparing a certain concentration standard solution containing 17 organochlorine pesticides; (2) examining standard solution under the propriety chromatogram conditions to obtain standard chromatogram graph; (3) preparing specimen: dipping specimen powder in ethyl acetate, ultrasonic extracting twice and purified and decoloring by PSA columella, decompressing and concentrating; (4) examining specimen under the same chromatogram conditions of reference substance to obtain chromatogram graph of specimen; (5) comparing the chromatogram graph between specimen and reference substance by preservation time contrast method to determine corresponding characteristic peak to calculate content by external standard method. The present invention is easy to application and dissemination, which has good purification effects to solve the problem of pollution of coloring matter to charomatograph. The present invention has high sensitivity, of which the recovery is 64%-110%; and good reproducibility and the warp is less than 15%.

The invention relates to a tea-leaf safety quick detection method, which mainly comprises that: after organophosphorus pesticide and organochlorine pesticide of tea-leaf are extracted by organic solvent, an organic phase is added with an oxidizing and degrading agent and a catalytic auxiliary, and is directly subjected to degradation for a certain period in the ultrasonic condition; inorganic degraded products in the organic phase are extracted; the content of the inorganics is detected by methods such as phospho-molybdenum blue and the like; and the content of organic pesticide components in a sample is calculated according to average proportion; therefore, the method realizes quick extraction and detection, avoids fussy operation such as heating, digestion, solvent volatilization and the like, and does not need expensive equipment such as a chromatograph and the like.

The invention provides a method for measuring residual quantity of organic chloride pesticides in traditional Chinese medicinal materials. A GPC-SPE-GC method is established, and the residual quantity of seventeen organic chloride pesticides in traditional Chinese medicinal materials is detected. The method comprises the following steps: extracting, performing preliminary GPC purification, performing solid phase extraction and binary purification, namely combining 200mg / 3mL of aminopropyl column NH2SPE and 250mg / 3mL of graphitized carbon black column GCB SPE, and sequentially purifying; and eluting according to a volume ratio of 8:2 of normal hexane to acetone solution. Compared with a traditional concentrated sulfuric acid purification method, the method disclosed by the invention has high sensitivity and safety, provides reliable detection means for safety production of traditional Chinese medicinal materials in China and safety supervision of exported foods and drugs and is worthy of popularization and application.

The invention belongs to the technical field of remediation works of organic contaminated soil, and discloses a novel method for remediating site soil contaminated by organo-chlorine. The method comprises the steps of preparing soil contaminated by an organo-chlorine pesticide into slurry soil mixed solution; adding certain amounts of citric acid, nanoscale zero-valent iron and calcium peroxide; and oscillating the materials for 3 h to 24 h, so as to enable the degradation rate of the organo-chlorine pesticide in soil to reach 85% or above. The method has the advantages that treatment efficiency is high, pollutants are completely degraded, waste of an oxidizing agent is low, technological conditions are simple, operation requirements are low, and the like, and the method is applicable to remediation of soil contaminated by the organo-chlorine pesticide.

The invention relates to a method for determining organic matter in demolition wastes. The method comprises the following steps: adding an acetone solution into the demolition wastes, and carrying out synchronous pretreatment extraction through ultrasonic-centrifugal coupling to obtain extraction liquid containing organic matter; purifying the extraction liquid by a silicone solid-phase extraction column; and finally, determining the content of organic matter in the demolition wastes by a gas chromatograph-mass spectrometer (GC-MS). The organic matter in the demolition wastes include polluted construction wastes with various grain sizes in industrial enterprises and organic chlorine pesticides, organic phosphorus pesticides and polycyclic aromatic hydrocarbon in soil. The method is high in determining speed, accurate in analysis result, low in pretreatment cost and easy to combine with the GC-MS and is suitably used for synchronously extracting polycyclic aromatic hydrocarbon and pesticides from the demolition wastes of the industrial enterprises and purifying and determining organic pollutants such as pesticides and polycyclic aromatic hydrocarbon.

The invention relates to a pesticides benzoepin artificial antigen-antibody and it's preparing method and application, which specifically relates to preparing small molecular compound artificial hapten, antigen and antibody of cyclopentacliene pesticides benzoepin and application of establishing immunity analysis method. It uses benzoepin derivation as hapten to connect separately with carrier protein like hemocyanin and horse radish peroxidase so as to synthesis artificial antigen and enzyme antigen. Artificial antigen becomes antibody though animal immunity, taking-up blood, antiserum and purity.

The invention discloses an eluent for soil polluted by organochlorine pesticides and a repair method by using the same. The eluent comprises 5-10%v / v petroleum ether with the boiling range of 60-90 DEG C, 10%v / v of normal propyl alcohol, and the balance of water. The method for repairing the soil polluted by the organochlorine pesticides comprises the steps of: screening polluted soil; adding theeluent with the volume 2 times relative to the soil, heating and stirring; separating upper-layer liquid in a stirrer, carrying out soil-water separation on a lower-layer soil suspending liquid system through plate frame pressure filtration to complete elution repair. When the eluent is adopted to elute the polluted soil with the organochlorine pesticide concentration of 10-20,000ppm, the remove rate on target pollutants all can reach about 80%, and the elution cost is about 600-2,500RMB / ton of soil along with the different pollution degrees. The method has an application prospect on repairing the soil of an area polluted by closed and relocated organochlorine pesticide production enterprises.