Tea-leaf safety quick detection method

A detection method, tea technology, applied in the preparation of test samples, analysis through chemical reaction of materials, material analysis through observation of the influence of chemical indicators, etc., can solve the complicated experimental process and high requirements for experimental personnel , Expensive gas chromatography equipment and other issues, achieve high sensitivity, simplify operation time, and simplify operation steps

Active Publication Date: 2009-12-30
厦门斯坦道生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a tea safety rapid detection method, the main purpose of which is to overcome the shortcomings of inaccurate testing by enzyme inhibition method, and overcome the shortcomings of expensive equipment, high requirements for experimenters and complicated experimental process of gas chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The present embodiment provides the rapid detection method of tea organophosphorus pesticide, and specific method is as follows:

[0023] 1. Extraction: Weigh 1-5 grams of tea samples to be tested in test tube I, add 2-10 ml of absolute ethanol, acetone, ethyl acetate or dichloromethane, and perform ultrasonic vibration for 3 minutes to obtain an extract.

[0024] 2. Degradation: Pour all the above extracts into the test tube II, add the organic phosphorus detection reagent, then place it in the sponge hole of the ultrasonic oscillator, and ultrasonically oscillate for 5-25 minutes. The organic phosphorus detection reagent is an oxidation degradation agent And ultrasonic degradation aids, the oxidative degradation agent is more than 1% of sodium peroxide, calcium peroxide, metal peroxide, alkaline earth metal peroxide, persulfate, hydrogen peroxide or one or more; ultrasonic oscillation power exceeds 60W , the shaking time is more than 5 minutes.

[0025] 3. Extraction...

Embodiment 2

[0030] The present embodiment provides the rapid detection method of tea organochlorine pesticide, and specific method is as follows:

[0031] 1. Extraction: Weigh 1-5 grams of tea samples into test tube I, add 2-10 ml of n-hexane or petroleum ether, ultrasonically oscillate for 3 minutes, then add 1-5 ml of water to test tube I, shake for a few times, and let it stand for 30 minutes. seconds, and then use an adjustable pipette to draw all the upper yellow extract into the test tube II.

[0032] 2. Degradation: Add an organic chlorine oxidative degradation agent composed of an oxidative degradation agent and an ultrasonic degradation aid to the test tube II, then place it in the sponge hole of an ultrasonic oscillator, and ultrasonically oscillate for 5-25 minutes, wherein the oxidative degradation agent is More than 1% of sodium peroxide, calcium peroxide, metal peroxide, alkaline earth metal peroxide, persulfate, hydrogen peroxide, or more than 1%, the ultrasonic oscillation...

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Abstract

The invention relates to a tea-leaf safety quick detection method, which mainly comprises that: after organophosphorus pesticide and organochlorine pesticide of tea-leaf are extracted by organic solvent, an organic phase is added with an oxidizing and degrading agent and a catalytic auxiliary, and is directly subjected to degradation for a certain period in the ultrasonic condition; inorganic degraded products in the organic phase are extracted; the content of the inorganics is detected by methods such as phospho-molybdenum blue and the like; and the content of organic pesticide components in a sample is calculated according to average proportion; therefore, the method realizes quick extraction and detection, avoids fussy operation such as heating, digestion, solvent volatilization and the like, and does not need expensive equipment such as a chromatograph and the like.

Description

technical field [0001] The invention relates to a tea safety detection method, in particular to a rapid detection method for organic phosphorus, organochlorine pesticides and lead in agricultural products such as tea. Background technique [0002] In recent years, the tea industry is generally healthy. Tea manufacturers are also constantly improving the scientific and technological content and output of their products, and striving to create green and organic brands. Production and export enterprises suffered relatively large losses, and even affected the reputation of regional tea brands. The quality and safety of tea are also related to the health of consumers. Since Japan launched the positive list, my country's exports of agricultural products such as fruits and vegetables have also been affected by similar reasons. [0003] At present, the rapid detection of tea safety mainly focuses on organophosphorus and carbamate pesticide residues, and the enzyme inhibition metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/34
Inventor 汤新华
Owner 厦门斯坦道生物科技有限公司
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