The invention discloses a fermented feed for promoting ruminants to efficiently utilize soluble proteins as well as a preparation method and application of the fermented feed, and belongs to the technical field of animal feeds. The fermented feed is prepared by fermenting a substrate consisting of
cottonseed meal,
corn flour and
bran with a compound
bacteria solution, and the substrate comprises the following components in percentage by
mass: 5-31% of
cottonseed meal, 2-31% of
corn flour and 38-93% of
bran; the
mass of the composite bacterial liquid is 8-10% of that of the substrate, and the composite bacterial liquid is prepared by compounding a
candida tropicalis bacterial liquid and an
enterococcus faecalis bacterial liquid according to a volume ratio of 1: 1, wherein the concentrations of the
candida tropicalis bacterial liquid and the
enterococcus faecalis bacterial liquid are both 108 CFU / mL. The preparation method comprises the following steps: crushing and sieving the substrate, mixing, adjusting the
water content to 40%, spraying the compound
bacteria liquid, uniformly mixing, and fermenting at a constant temperature of 30 DEG C. According to the invention, the substrate ratio and the
fermentation process are optimized, the utilization efficiency of soluble
protein by ruminants is improved, the
rumen fermentation environment is improved, meanwhile, the
nitrogen emission is reduced, and
economic benefits and
environmental protection values are achieved in
animal production.