14results about How to "Improve community structure" patented technology

The remediation method for the moderately and severely cadmium-polluted rice field aims at strictly controlled cadmium-polluted rice fields, and is divided into two stages of strict control and safe utilization for remediation. In the strict management and control stage, an activating agent is applied to the soil in spring, then microalgae are inoculated, cadmium-enriched plants are planted, the plants are collected after being full of the microalgae, the plants are harvested when the plants grow to the enrichment extreme value, stubbles are reserved for growth, and the steps are repeated till winter; applying an activating agent in winter, planting green manure, and harvesting in spring in the next year; the process is cyclically carried out until the cadmium content of the soil is reduced to a safe utilization level. Entering a safe utilization stage, applying an activating agent in spring, inoculating nitrogen-fixing cyanobacteria, collecting the cyanobacteria after the cyanobacteria overgrow, and repeating the steps until the rice seedlings are transplanted; applying nitrogen-fixing blue-green algae, and planting rice; applying an activating agent after rice harvesting, planting winter green manure, and harvesting in next spring; the process is cyclically carried out until the cadmium content of the soil is reduced to a preferential protection level. The method is low in cost, high in remediation efficiency and good in effect, and a new way is provided for cadmium-polluted rice field remediation.

The invention relates to the technical field of constructed wetlands, in particular to a constructed wetland iron-carbon-manganese composite filler capable of efficiently removing nitrogen and phosphorus. According to the filler, the manganese ore layer is arranged behind the iron-carbon micro-electrolysis layer, the microenvironment in the iron-carbon micro-electrolysis constructed wetland is adjusted through manganese ore, iron and manganese exist at the same time and can serve as electron donors for microbial denitrification, the effect is better than that of single use of iron, meanwhile, the oxidation capacity of high-valence manganese ions is utilized, and the denitrification efficiency is improved. The ammonia nitrogen in the iron oxide carbon wetland is inhibited or re-oxidized, so that the total nitrogen removal capability of the wetland is enhanced, and efficient nitrogen removal is realized; the manganese ore is large in specific surface area and can adsorb iron ions in water, iron and manganese separated out of the water are effectively removed, and the problem that the dissolution concentration of the iron ions is high is solved; phosphate radicals are adsorbed and removed mainly through ligand exchange with hydroxyl on the surface of manganese nodule, iron ions and ferrous ions dissolved out from the scrap iron are combined with influent phosphate to form precipitates such as iron phosphate, and a very good effect on removal of total phosphorus is achieved.

The invention provides an improvement method for tobacco field soil in a high-altitude cold area, and belongs to the technical field of tobacco field soil improvement. The improvement method provided by the invention comprises the following steps: (1) in the later period of tobacco leaf picking or after tobacco leaf harvesting and soil preparation, spreading leguminous green manure, and after 10-30 days, spreading cruciferous green manure; 2, 25-35 days before tobacco transplanting, green manure is turned over and returned to the field, and meanwhile biochar is applied; and (3) preparing soil and ridging, applying a special fertilizer for tobacco, transplanting the tobacco, and applying a microbial agent when the tobacco is transplanted. According to the improvement method provided by the invention, the green manure green-pressing biomass can be remarkably improved, the soil fertility is improved, the activity and function of soil microorganisms are improved, the yield and quality of tobacco leaves are improved, the sustainable development of tobacco leaf production is promoted, and remarkable social benefits and economic benefits are generated.

The invention discloses a preparation method of fermented radix fici simplicissimae chickpeas with a probiotic function. The preparation method comprises the following steps: 1, cleaning chickpeas, and soaking overnight; step 2, draining water, adding radix fici simplicissimae, white granulated sugar and water, and cooking; step 3, removing the radix fici simplicissimae and water after cooking is completed, and naturally cooling the radix fici simplicissimae; 4, inoculating activated lactic acid bacteria, and performing constant-temperature fermentation; and step 5, collecting the fermented radix fici simplicissimae and chickpeas, and performing low-temperature preservation. The obtained fermented radix fici simplicissimae chickpeas contain various short-chain fatty acids generated in the lactic acid bacteria fermentation process, have the special flavor of radix fici simplicissimae and lactic acid bacteria fermentation, and have certain probiotic health-care functions.

The invention belongs to the technical field of water body in-situ remediation, and relates to a method for improving the self-cleaning capacity of a water body by utilizing sustained-release particles. According to the method, slow-release particles wrapped by dialysis bags are added into a water body, the release rate of a carbon source is controlled, and the nitrogen and phosphorus removal capacity of native microorganisms is activated. Compared with the prior art, the slow-release particles are wrapped by the dialysis bag, so that microorganisms are prevented from being attached to the particles, the carbon source release rate is controlled, and the microbial community structure is directionally induced; and the method has the remarkable advantages of simplicity, continuous and stable effect, no secondary pollution, environment friendliness and the like.

This invention discloses an organic fertilizer for increasing guava yield in saline-alkali land and its preparation method, relating to the field of biotechnology. The active ingredients of this organic fertilizer include *Bacillus horikoshii* ACCC 01061, *Bacillus safensis* CICC 21743, *Sporidiobolus salmonicolor* CICC 32895, *Trichoderma koningii* CICC 13006, and *Trichoderma longibrachiatum* ACCC 32095. This organic fertilizer can improve the yield per guava tree and the quality of the fruit grown in saline-alkali land, and has significant market application value.

The invention discloses a composite microbial agent containing pseudomonas and application thereof, and belongs to the technical field of agricultural microorganisms. The composite microbial agent disclosed by the invention comprises pseudomonas synthetic flora and N-acetyl-L-tryptophan, wherein the N-acetyl-L-tryptophan can be used for effectively inhibiting the growth of pathogenic bacteria and effectively improving the colonization capability of pseudomonas in soil; the pseudomonas and the N-acetyl-L-tryptophan are combined for use, so that the relative abundance of beneficial bacteria in continuous cropping soil can be improved, and the relative abundance of harmful bacteria can be reduced; the positive correlation proportion of continuous cropping soil microorganism interaction is increased; the apple rhizosphere microenvironment is remodeled, and the growth of apple seedlings in the continuous cropping soil is promoted, so that the prevention and treatment effect on the apple continuous cropping obstacles is remarkably improved.

A method for recycling and repairing heavy metal contaminated soil by comprehensively utilizing willow and its derivatives, using willow and organic eluent to synergistically treat heavy metal contaminated sites, improving the efficiency of using willow to repair heavy metal contaminated sites, and shortening the repair time to 2-3 years, while traditional willow repair of heavy metal contaminated sites usually takes 6-10 years, greatly shortening the time for willow to repair heavy metal contaminated sites. At the same time, waste such as trees and leaves generated in the process of plant repair of heavy metal contaminated sites are effectively utilized, and the coarse wood block particles, fine particle compost, biochar and organic eluent obtained by fermentation are reused in site repair, so that they are recycled and reused, avoiding air pollution caused by traditional treatment methods.

The utility model discloses a kind of harmful microbial communities in sludge regulating device, belong to bottom mud processing technical field, its technical scheme main point is, including ship and placing in ground air solution complex oxygen system, the ship is provided with lifter, the lifter is connected with adjusting part, the adjusting part includes enclosed cover, the top of enclosed cover is provided with driving motor, driving motor's output shaft is installed with rotating rod, the end of rotating rod is provided with pawl, rotating rod is provided with threaded section, threaded section is spin-connected with moving disc, moving disc separates the enclosed cover inside into placement area and stirring area, the radius of stirring area is greater than the radius of placement area, water purifying agent is placed in the placement area, enclosed cover is provided with slide bar, the moving disc is slidably connected with the slide bar. This kind of scheme closed fixed point processing and optimize the community structure of microorganism in bottom mud, secondary pollution does not appear, effectively promote water quality.

The invention provides an earthworm protein extract as well as an extraction method and application thereof. The earthworm protein extract can be applied to agricultural production. The extraction method comprises the following steps: preparing cleaned earthworms into earthworm homogenate; adding water into the earthworm homogenate, and carrying out solid-liquid separation to obtain an extracting solution containing earthworm protein; then protease is added for enzymolysis; and performing purification treatment on the extracting solution after enzymolysis to obtain the earthworm protein extract. The extraction method of the earthworm protein extract is simple, extraction parameters are controllable, and the extracted earthworm protein is high in extraction rate and purity. The earthworm protein extract is rich in eighteen amino acids, various nucleotides, FA proteins and other components, can provide essential raw materials for plant metabolism, is beneficial to plant tissues to coordinate cell division and differentiation activities, can effectively improve the yield, quality and resistance of crops, and has the effects of improving the soil structure, improving the soil fertility and the like.

The invention discloses a fermented feed for promoting ruminants to efficiently utilize soluble proteins as well as a preparation method and application of the fermented feed, and belongs to the technical field of animal feeds. The fermented feed is prepared by fermenting a substrate consisting of cottonseed meal, corn flour and bran with a compound bacteria solution, and the substrate comprises the following components in percentage by mass: 5-31% of cottonseed meal, 2-31% of corn flour and 38-93% of bran; the mass of the composite bacterial liquid is 8-10% of that of the substrate, and the composite bacterial liquid is prepared by compounding a candida tropicalis bacterial liquid and an enterococcus faecalis bacterial liquid according to a volume ratio of 1: 1, wherein the concentrations of the candida tropicalis bacterial liquid and the enterococcus faecalis bacterial liquid are both 108 CFU / mL. The preparation method comprises the following steps: crushing and sieving the substrate, mixing, adjusting the water content to 40%, spraying the compound bacteria liquid, uniformly mixing, and fermenting at a constant temperature of 30 DEG C. According to the invention, the substrate ratio and the fermentation process are optimized, the utilization efficiency of soluble protein by ruminants is improved, the rumen fermentation environment is improved, meanwhile, the nitrogen emission is reduced, and economic benefits and environmental protection values are achieved in animal production.

The invention discloses a phosphorus-solubilizing fungus soil remediation agent as well as a preparation method and application thereof, and belongs to the technical field of soil remediation. The phosphorus-solubilizing fungus soil remediation agent comprises micro-nano bone black and a penicillium deserticola solid fungicide. The preparation method comprises the following steps: pyrolyzing pig bones, and grinding to obtain the micro-nano bone black; the method comprises the following steps: coating a solid culture medium with penicillium deserticola for culturing to obtain a solid plate, washing the solid plate after spores are mature to obtain a spore liquid, mixing a solid carrier with sterile water, then adding a solid fungicide protective agent, and finally adding the spore liquid to obtain the penicillium deserticola solid fungicide. The phosphorus-solubilizing fungus soil remediation agent provided by the invention not only can effectively promote dissolution of insoluble phosphate, but also can fix heavy metal cadmium (Cd) in soil, has a very good application value for farmland Cd pollution remediation and environmental protection, is green and environment-friendly, can comprehensively improve soil fertility, and creates a good nutritional condition for crop growth.

The invention discloses an organic fertilizer for improving yield of guava planted in saline-alkali soil and a preparation method thereof, and relates to the technical field of biology. The active ingredients of the organic fertilizer comprise bacillus horikoshii (ACCC) 01061, bacillus saferae (CICC) 21743, Sporidiobolus salmoninensis (CICC) 32895, Trichoderma koningii (CICC) 13006 and Trichoderma longibrachiatum (ACCC) 32095, and the active ingredients of the organic fertilizer comprise bacillus horikoshii (ACCC) 01061, bacillus saferae (CICC) 21743, Sporidiobolus salmoninensis (CICC) 32895, Trichoderma koningii (CICC) 13006 and Trichoderma longibrachiatum (ACCC) 32095. The organic fertilizer can improve the single-plant yield and fruit quality of guavas planted in saline-alkali soil, and has important market application value.

This invention discloses a compound microbial agent containing Pseudomonas and its application, belonging to the field of agricultural microbiology technology. The compound microbial agent of this invention includes a Pseudomonas synthetic flora and N-acetyl-L-tryptophan. N-acetyl-L-tryptophan can effectively inhibit the growth of pathogens and effectively improve the colonization ability of Pseudomonas in the soil. The combined use of Pseudomonas and N-acetyl-L-tryptophan can increase the relative abundance of beneficial bacteria and decrease the relative abundance of harmful bacteria in continuously cropped soils; increase the positive correlation ratio of microbial interactions in continuously cropped soils; reshape the rhizosphere microenvironment of apples; and promote the growth of apple seedlings in continuously cropped soils, thereby significantly improving the control effect on continuous cropping obstacles in apples.