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Polyglutamic acid gel micrococcusagent and application thereof

A technology of polyglutamic acid gel and polyglutamic acid, which is applied in the field of microorganisms, can solve the problems of unfavorable cell nutrition and metabolite transportation, low effective bacteria content, and easy inactivation of effective bacteria, so as to achieve rapid Effects of colonization and slow release, improvement of nutrient absorption, and promotion of rapid proliferation

Inactive Publication Date: 2019-10-18
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the large size of traditional hydrogel cell carriers is not conducive to the delivery of nutrients and metabolites of all cells in the hydrogel, and the cells inside the hydrogel will die due to factors such as hypoxia and insufficient nutrition.
[0006] During the cultivation of crops, various soil-borne diseases such as dead seedlings, rotten roots, green withering, wilting, wilting, root rot, stem base rot, damping, etc. often plague farmers, and the solution is mostly to use pesticides or microorganisms Bacteria agents, and due to the limitation of the existing technical level, the microbial inoculum agents currently on the market have defects such as low number of effective bacteria and easy inactivation of effective bacteria, and the effect is relatively slow after application
At present, there is no report on the use of polyglutamic acid slow-release microspheres to embed microbial agents, and there is no report on the prevention and treatment of the above-mentioned agricultural diseases by microbial agent microspheres.

Method used

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  • Polyglutamic acid gel micrococcusagent and application thereof
  • Polyglutamic acid gel micrococcusagent and application thereof
  • Polyglutamic acid gel micrococcusagent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 polyglutamic acid slow-release microsphere (does not contain microorganism)

[0044] Get 1g (7.8mmol carboxylate) γ-polyglutamic acid (molecular weight 2000kDa) and dissolve it in 20mL deionized water, after stirring to fully dissolve, adjust the pH value of γ-polyglutamic acid solution with dilute hydrochloric acid (0.1wt% concentration) is 4.5. Then, slowly add 1.02ml (7.8mmol) glycidyl methacrylate dropwise at room temperature and stir to dissolve evenly. During the dropwise addition, use dilute hydrochloric acid (0.1% concentration) to control the pH value of the reaction solution at 4.5. The reaction was stirred for 8 hours. After the reaction, use a dialysis bag with a molecular cut-off of 3500Da to dialyze at room temperature for 5 days, and finally freeze-dry to obtain a white flocculent gamma-polyglutamic acid solid modified with glycidyl methacrylate.

[0045] Add Span 80 into hexadecane and stir well to form a continuous phas...

Embodiment 2

[0047] The fermentation of embodiment 2 penicillium aspenii

[0048] Prepare the microbial inoculum of the present invention as follows:

[0049] Wherein, the fermentation and culture conditions of Penicillium aspenicillium are as follows: after solid-state fermentation of Penicillium aspenicillium CGMCC No.17198, washing with normal saline, and filtering with 4 layers of lens-cleaning paper to obtain a spore suspension. The solid-state fermentation medium is mixed nutrients and water, and the mixed nutrients are any one or more of wheat bran, cassava flour, cassava residue, and straw, preferably 75g of wheat bran, 25g of straw, 80mL of water, and the culture medium is sterilized. The bacteria were solid-state fermented in a 20cm*40cm air-permeable bag, and the inoculation amount was 1×10 Penicillium aspenicillium spore suspension 7 20mL, the fermentation condition is 7 days, 28°C. The spores were washed with sterile water to make a spore suspension, counted by a hemocytome...

Embodiment 3

[0050] The fermentation of embodiment 3 Pantoea camelini, bacillus subtilis, jelly-like bacillus, bacillus amyloliquefaciens

[0051] Inoculate Pantoea camelini CGMCC NO.15525 stored on a slant at 4°C on eggplant-shaped culture flask medium (ie, activation medium) and culture at 30°C for 16 hours. Pick the strains on the activated medium, inoculate them in the fermentation medium, culture at 30°C for 40 hours, place the fermentation broth or the concentrated solution of the fermentation broth in a cold storage at 4°C for 48 hours at low temperature, and measure it by plate coating. The number of viable bacteria in the bacterial fermentation broth can reach 1×10 10 CFU / mL or more. The medium in the eggplant-shaped culture bottle is LB medium; the components of the fermentation medium are: sucrose 50g / L, beef extract 3g / L, NaCl 8g / L, peptone 8g / L, pH 6.0-9.0.

[0052] Activate the Bacillus subtilis CCTCC NO:M 2016264, Bacillus jelly-like CCTCC NO:M2016265, and Bacillus amyloli...

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Abstract

The invention discloses a polyglutamic acid gel micrococcus agent.A microorganism, a glycidyl methacrylate modified gama-polyglutamic acid, a photoinitiator, and water are used as dispersion phases, ahexadecane and a span 80 are used as continuous phases, droplet-based microfluidics technology is adopted to prepare microspheres, the sizes of themicrospheresare between 100 to 500 [mu]m. The invention further discloses preparation method and application of the polyglutamic acid gel micrococci agent. According to the polyglutamic acid gel microspheres,anembedding microbial agent can be released,the effective period of the high effective viable count can be prolonged, high activity biological function is realized, colonization of the functional microbial agent in the soil is advantageous, avariety of crop diseasescan be effectivelyprevented and controlled, the using amounts of chemical pesticides and chemical fertilizers are decreased, biological control and chemical control areeffectively combined, strengths are taken to offset weaknesses, integrated crop prevention and control is realized, and time and effort are saved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a polyglutamic acid gel micrococcal agent and its application. Background technique [0002] Soil is the foundation of agricultural planting, and soil issues are related to the national economy and people's livelihood. Since the reform and opening up, with the rapid development of my country's industrial modernization and agricultural modernization process, especially the excessive use of pesticides and fertilizers, sewage irrigation, continuous cropping management, etc. have caused the deterioration of farmland soil to become more and more serious, and it has accumulated year by year. The deterioration of farmland soil is mainly reflected in soil compaction, soil salinization, soil flora imbalance, continuous cropping obstacles, and increased concentration of heavy metals. The deterioration of farmland soil has become a bottleneck problem restricting the sustainable deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05G3/00C05G5/00A01G31/00A01C21/00A23K10/18A23K20/147A23K40/30A23L29/00A23P10/30C12N11/08C12N11/04C12N1/04C12R1/80C12R1/125C12R1/07C12R1/01
CPCC05G3/00C05F11/08A01G31/00A01C21/00A23K10/18A23K20/147A23K40/30A23L29/065A23L29/045A23P10/30C12N11/08C12N11/04C12N1/04A23V2002/00C05G5/35C05G3/80A23V2200/224
Inventor 徐虹彭雯杰詹伊婧王瑞杨凯冯小海李莎许宗奇
Owner NANJING UNIV OF TECH
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