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Bacillus subtilis and application thereof

The technology of Bacillus subtilis and bacteria is applied in the field of microorganisms, which can solve the problems of large variation in pathogenicity of Fusarium wilt, long breeding cycle of disease-resistant varieties, and great harm to human body, so as to reduce the use of pesticides, facilitate pollution-free production, Good long-lasting effect

Active Publication Date: 2019-08-02
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Agricultural control, such as crop rotation and stubble replacement, can reduce the number of pathogenic bacteria in the soil, but it cannot be widely used due to the shortage of agricultural land in our country. Although the selection of disease-resistant varieties and strengthening field management measures also have a certain effect on disease prevention and control, However, the breeding cycle of disease-resistant varieties is long, and the pathogenicity of Fusarium wilt is highly variable, and the resistance is easy to disappear
Although chemical control is fast and effective, it is easy to cause pathogenic bacteria to develop drug resistance, cause environmental pollution and pesticide residues, and cause great harm to the human body. It does not comply with the national policy of green agriculture, so it is restricted for use

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the inhibitory effect of Bacillus subtilis described in the present invention on 8 kinds of crop wilt pathogenic fungi

[0031] A. For testing pathogenic fungi:

[0032] Pathogenic fungi include Fusarium wilt of tomato, Fusarium wilt of cucumber, Fusarium wilt of melon, Fusarium wilt of watermelon, Fusarium wilt of cotton, Fusarium wilt of banana, Fusarium stevia wilt, and Fusarium cauliflower.

[0033] B. Test method:

[0034]In this experiment, the plate confrontation method was adopted, and 8 kinds of crop fusarium wilt pathogens were respectively inoculated on the PDA medium (200g potato, 20g glucose, 15g agar and 1000mL distilled water, pH6.5-7.0) plate for activation. Bacteria puncher uniformly punches circular bacterial blocks with a diameter of 8 mm from the outer edge of the colony. Inoculate the mycelium block at the center of a new PDA plate, inoculate the Bacillus subtilis of the present invention at an equidistant distance of 25 mm from the ...

Embodiment 2

[0040] Embodiment 2: the mensuration of bacillus subtilis bacteriostatic spectrum of the present invention

[0041] A. For testing pathogenic fungi:

[0042] Including 26 kinds of fungal pathogens and 3 kinds of bacterial pathogens except Fusarium wilt, all of which were isolated and preserved by Jingbo Chemical Research Institute in the Yellow River Delta. See Table 2 for details.

[0043] B. Test method:

[0044] Method 1: The fungal pathogen adopts the plate confrontation method. Crop pathogenic bacteria were inoculated on PDA medium (200g potato, 20g glucose, 15g agar and 1000mL distilled water, pH6.5-7.0) plate respectively for activation, after the fungus overgrown the plate, use a sterilized hole puncher to spread evenly from the outer edge of the colony. be beaten into round bacterium blocks with a diameter of 8mm. Mycelium block is inoculated in the center of new PDA plate, inoculates Bacillus subtilis described in the present invention at its four weeks away from ...

Embodiment 3

[0051] Embodiment 3: Indoor potted control effect experiment of tomato fusarium wilt

[0052] Damage the root hairs of tomato seedlings at the 2-4 true leaf stage, and then in the pathogenic bacteria spore liquid (1 × 10 6 spores / mL) soaked for 20min, transplanted into pots with vermiculite, 5 plants per pot, and 20mL of pathogenic bacteria spore liquid (1×10 6 spore / mL), after 24h, the roots of the experimental group were irrigated with 20mL of antagonistic bacteria liquid (bacteria content was 10 8 cfu / mL); taking 500-fold diluted 50% carbendazim as the drug control, that is, the drug control treatment is to pour 500-fold diluted 50% carbendazim on the roots of tomato seedlings that have been inoculated with tomato Fusarium wilt, 20 mL per plant; Tomato seedlings inoculated with Fusarium wilt of tomato treated with clean water were used as blank control. For each treatment of 10 plants, the routine management was carried out, and the condition was investigated after 35 day...

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Abstract

The invention relates to the technical field of microorganisms, and discloses bacillus subtilis and application thereof. The preservation number of the bacillus subtilis is CGMCC No. 17357. The strainof the bacillus subtilis disclosed by the invention has a wide antibacterial spectrum; the inhibitory effect on fusarium wilt germs of multiple crops reaches up to 75% or above; moreover, the inhibitory effect on other 26 kinds of fungal pathogenic bacteria and 2 kinds of bacterial pathogenic bacteria is obvious; field planting in soil is easy; pesticide resistance is less liable to generate; thedurability of pesticide effect is good; the usage amount of pesticide can be effectively reduced; pollution-free production of the crops is facilitated; the expenditure of farmers is reduced.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a bacillus subtilis and its application. Background technique [0002] Crop wilt is a soil-borne disease caused by Fusarium oxysporum, which can harm various economic crops such as melons, beans, and solanaceous fruits. Because Fusarium oxysporum has continuously evolved new physiological races, and its Chlamydospores can survive in the soil for a long time, making the control of Fusarium wilt very difficult and seriously affecting crop production. Taking Cucurbit Fusarium wilt as an example, diseased fields generally reduce production by 20%-30%, and serious fields can reach 50%-60% %, or even extinct. Crop wilt has a wide range, deep damage, and difficult control, which seriously affects crop production in our country. Therefore, effective control of fusarium wilt has become an urgent task to increase crop yield in our country. [0003] At present, the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00A01P3/00C12R1/125
CPCC12N1/20A01N63/00C12R2001/125C12N1/205A01N63/10
Inventor 张萧萧马韵升吴文雷郭南南张心青杨传伦杨丹丹倪建龙蔡倩倩
Owner CHAMBROAD CHEM IND RES INST CO LTD
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