Bacillus subtilis and application thereof

The technology of Bacillus subtilis and bacteria is applied in the field of microorganisms, which can solve the problems of large variation in pathogenicity of Fusarium wilt, long breeding cycle of disease-resistant varieties, and great harm to human body, so as to reduce the use of pesticides, facilitate pollution-free production, Good long-lasting effect

Active Publication Date: 2019-08-02
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Agricultural control, such as crop rotation and stubble replacement, can reduce the number of pathogenic bacteria in the soil, but it cannot be widely used due to the shortage of agricultural land in our country. Although the selection of disease-resistant varieties and strengthening field management measures also have a certain effect on disease prevention and control, However, the breeding cycle of disease-resistant

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1: The inhibitory effect of the Bacillus subtilis of the present invention on the pathogenic fungi of Fusarium wilt of 8 crops

[0031] A. Test pathogenic fungi:

[0032] Pathogenic fungi include tomato fusarium, cucumber fusarium, melon fusarium, watermelon fusarium, cotton fusarium, banana fusarium, stevia fusarium, and cauliflower fusarium, all of which are isolated and preserved by the Yellow River Delta Jingbo Chemical Research Institute.

[0033] B. Test method:

[0034] In this experiment, the plate confrontation method was adopted to inoculate 8 species of Fusarium wilt bacteria into PDA medium (200g potato, 20g glucose, 15g agar and 1000mL distilled water, pH6.5-7.0) plates to activate them, and kill them after the fungi grow up on the plates. The punch of the bacteria evenly beats the outer edge of the colony into circular clumps with a diameter of 8mm. The mycelium block was inoculated in the center of a new PDA plate, and the Bacillus subtilis of the presen...

Example Embodiment

[0040] Example 2: Determination of the antibacterial spectrum of Bacillus subtilis of the present invention

[0041] A. Test pathogenic fungi:

[0042] Including 26 kinds of fungal pathogens and 3 kinds of bacterial pathogens except Fusarium wilt, all of which are separated and preserved by the Yellow River Delta Jingbo Chemical Research Institute. See Table 2 for details.

[0043] B. Test method:

[0044] Method 1: The fungal pathogens adopt the plate confrontation method. Crop pathogens were inoculated into PDA medium (200g potato, 20g glucose, 15g agar and 1000mL distilled water, pH 6.5-7.0) plates for activation. After the fungi overgrown on the plate, use a sterile punch to evenly spread from the outer edge of the colony Smashed into round clumps with a diameter of 8mm. The mycelium block is inoculated in the center of a new PDA plate, and the Bacillus subtilis of the present invention is inoculated at an equal distance of 25mm from the center around it, and the growth characte...

Example Embodiment

[0051] Example 3: Indoor potted tomato control experiment

[0052] Injury the root hairs of tomato seedlings at the true leaf stage of 2-4, and then apply the pathogenic spore solution (1×10 6 Spores / mL) soak for 20min, transplant to a pot filled with vermiculite, 5 plants per pot, irrigate 20mL pathogen spore solution (1×10 6 Spores / mL). After 24 hours, the experimental group was irrigated with 20 mL of antagonistic bacterial solution (the bacterial content was 10 8 cfu / mL); 50% carbendazim diluted 500 times is used as the medicament control, that is, the medicament control treatment is to water 50% carbendazim diluted 500 times to the roots of tomato seedlings that have been inoculated with tomato fusarium wilt, 20 mL per plant; The tomato seedlings that had been inoculated with Fusarium wilt with clean water were used as blank control. Every 10 plants are treated, routinely managed, and the disease condition is investigated after 35 days of growth, and the control effect is cal...

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Abstract

The invention relates to the technical field of microorganisms, and discloses bacillus subtilis and application thereof. The preservation number of the bacillus subtilis is CGMCC No. 17357. The strainof the bacillus subtilis disclosed by the invention has a wide antibacterial spectrum; the inhibitory effect on fusarium wilt germs of multiple crops reaches up to 75% or above; moreover, the inhibitory effect on other 26 kinds of fungal pathogenic bacteria and 2 kinds of bacterial pathogenic bacteria is obvious; field planting in soil is easy; pesticide resistance is less liable to generate; thedurability of pesticide effect is good; the usage amount of pesticide can be effectively reduced; pollution-free production of the crops is facilitated; the expenditure of farmers is reduced.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a bacillus subtilis and its application. Background technique [0002] Crop wilt is a soil-borne disease caused by Fusarium oxysporum, which can harm various economic crops such as melons, beans, and solanaceous fruits. Because Fusarium oxysporum has continuously evolved new physiological races, and its Chlamydospores can survive in the soil for a long time, making the control of Fusarium wilt very difficult and seriously affecting crop production. Taking Cucurbit Fusarium wilt as an example, diseased fields generally reduce production by 20%-30%, and serious fields can reach 50%-60% %, or even extinct. Crop wilt has a wide range, deep damage, and difficult control, which seriously affects crop production in our country. Therefore, effective control of fusarium wilt has become an urgent task to increase crop yield in our country. [0003] At present, the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00A01P3/00C12R1/125
CPCC12N1/20A01N63/00C12R2001/125C12N1/205A01N63/10
Inventor 张萧萧马韵升吴文雷郭南南张心青杨传伦杨丹丹倪建龙蔡倩倩
Owner CHAMBROAD CHEM IND RES INST CO LTD
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