Medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of medicago pseudomonas strain
A technology of Pseudomonas and deaminase, which is applied in the field of Pseudomonas alfalfa, can solve the problems of inability to sow seeds, high cost, and long production cycle of bacterial agents, so as to promote plant nutrient absorption, promote growth and development, and improve plant health. The effect of resistance
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specific Embodiment approach 1
[0019] Specific embodiment one: A Pseudomonas alfalfa (Pseudomonas sp.) MJM-9 producing ACC deaminase in this embodiment is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation address is Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District, the preservation date is June 26, 2012, and the preservation number is CGMCC NO.6294.
[0020] In this embodiment, Pseudomonas sp. (Pseudomonas sp.) MJM-9 is a Gram-negative bacterium, which forms round, opaque, light yellow, smooth protrusions, neat edges, and sticky colonies on the ADF medium (such as figure 1 shown).
[0021] Perform Gram staining, catalase, methyl red, acetylmethylmethanol, starch on the isolated Pseudomonas sp. Detection and identification of hydrolysis, indole, citrate action physiological and biochemical experiments. The results showed that Pseudomonas sp. MJM-9 was a Gram-negative bacterium, and it was positive for catalase, pos...
specific Embodiment approach 2
[0025] Embodiment 2: A Pseudomonas meliloti producing ACC deaminase in this embodiment is used as bacterial fertilizer for crop cultivation.
[0026] The Pseudomonas sp. MJM-9 of this embodiment belongs to the plant growth-promoting rhizobacteria (PGPR for short), and the plant growth-promoting rhizobacteria refers to bacteria that live freely in the soil or grow A class of beneficial fungi in the rhizosphere of plants that can promote plant growth, prevent diseases, and increase crop yields. PGPR of ACC (1-aminocyclopropane-1-carboxylic acid 1-aminocyclopropane-1-carboxylate, referred to as ACC) deaminase, ACC deaminase is an exogenous biomass-promoting substance that can effectively reduce the content of ethylene under stress. Under adversity conditions, it can slow down the accumulation of ethylene in plants, promote plant growth, especially the growth of plant roots, significantly improve the stress resistance and fertilizer efficiency utilization rate of crops, and increa...
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