Medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of medicago pseudomonas strain

A technology of Pseudomonas and deaminase, which is applied in the field of Pseudomonas alfalfa, can solve the problems of inability to sow seeds, high cost, and long production cycle of bacterial agents, so as to promote plant nutrient absorption, promote growth and development, and improve plant health. The effect of resistance

Active Publication Date: 2012-12-19
HARBIN NORMAL UNIVERSITY
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  • Summary
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  • Application Information

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Problems solved by technology

However, some fungi cannot be purely cultured, the production cycle of the fungal agent is long, the cost is large, the inoculation technology is difficult to implement, and it cannot be so

Method used

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  • Medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of medicago pseudomonas strain
  • Medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of medicago pseudomonas strain
  • Medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of medicago pseudomonas strain

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specific Embodiment approach 1

[0019] Specific embodiment one: A Pseudomonas alfalfa (Pseudomonas sp.) MJM-9 producing ACC deaminase in this embodiment is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation address is Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District, the preservation date is June 26, 2012, and the preservation number is CGMCC NO.6294.

[0020] In this embodiment, Pseudomonas sp. (Pseudomonas sp.) MJM-9 is a Gram-negative bacterium, which forms round, opaque, light yellow, smooth protrusions, neat edges, and sticky colonies on the ADF medium (such as figure 1 shown).

[0021] Perform Gram staining, catalase, methyl red, acetylmethylmethanol, starch on the isolated Pseudomonas sp. Detection and identification of hydrolysis, indole, citrate action physiological and biochemical experiments. The results showed that Pseudomonas sp. MJM-9 was a Gram-negative bacterium, and it was positive for catalase, pos...

specific Embodiment approach 2

[0025] Embodiment 2: A Pseudomonas meliloti producing ACC deaminase in this embodiment is used as bacterial fertilizer for crop cultivation.

[0026] The Pseudomonas sp. MJM-9 of this embodiment belongs to the plant growth-promoting rhizobacteria (PGPR for short), and the plant growth-promoting rhizobacteria refers to bacteria that live freely in the soil or grow A class of beneficial fungi in the rhizosphere of plants that can promote plant growth, prevent diseases, and increase crop yields. PGPR of ACC (1-aminocyclopropane-1-carboxylic acid 1-aminocyclopropane-1-carboxylate, referred to as ACC) deaminase, ACC deaminase is an exogenous biomass-promoting substance that can effectively reduce the content of ethylene under stress. Under adversity conditions, it can slow down the accumulation of ethylene in plants, promote plant growth, especially the growth of plant roots, significantly improve the stress resistance and fertilizer efficiency utilization rate of crops, and increa...

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Abstract

The invention discloses a medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of the medicago pseudomonas strain, and relates to a medicago pseudomonas strain and application thereof. The pseudomonas (pseudomonas sp.) MJM-9 is preserved in the general microorganism center of the China microorganism culture preservation management committee in 26th July, 2012, and the preservation number of the pseudomonas MJM-9 is CGMCC NO.6294. The medicago pseudomonas strain containing the ACC deaminase is used as bacterial manure for growing crops. The medicago pseudomonas strain can generate the ACC deaminase, secrete auximone IAA (indole acetic acid), synthesize siderophores, reduce the concentration of ethylene inside a plant under the condition of environmental stress, promote the plant to grow fast, particularly promote growth and development of a root system of the plant, and increase utilization rate of fertilizers. Besides, when the strain is applied to agricultural production, growth of plants can be promoted, yield of crops can be increased, and stress resistance of the crops is improved.

Description

technical field [0001] The invention relates to a strain of Pseudomonas alfalfa and its application. Background technique [0002] Alfalfa (Medicago spp.) is a perennial leguminous forage widely planted in the world. It has the reputation of "the king of forage" and has a cultivation history of more than 2,000 years in my country. Alfalfa has high nutritional value, rich in crude protein, vitamins and minerals, relatively complete amino acid composition, salt-alkali resistance, drought resistance, barren resistance, strong growth adaptability, is a high-quality forage grass, and can improve land utilization at the same time , Improve soil fertility. With wide ecological adaptability and stable productivity, it plays a huge role in my country's agricultural production and ecological economic construction. [0003] Alfalfa is a moderately saline-tolerant legume that grows well in neutral or slightly saline soils. When the soil salt content is 0.2-0.3% and the pH value is 7....

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/38
Inventor 郭长虹马嘉敏史煦涵蔡洪生谢宝明
Owner HARBIN NORMAL UNIVERSITY
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