Preparation method of medicinal platycodon grandiflorum growth-promoting microbial inoculum
By combining the high-seed bacterial strain and the pre-embedded bacterial promoter technology, using the symbiotic relationship between the degradable packaging body and rhizobia, the bacterial promoter agent is directly applied to the root of the Platycodon, which solves the slow infiltration of the bacterial promoter agent in the prior art and the slow and stable bacterial promoter agent in the prior art. The problem of excessive loss has achieved rapid and efficient growth of Platycodon and improved soil fertility.
Patent Information
- Application Number
- CN202411144158.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-20
- Publication Date
- 2024-12-06
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing bacterial promoters seep slowly after being applied above the soil, have low efficiency and a lot of loss, and cannot fully reach the rhizosphere of Platycodon, resulting in problems of growth disorders and reduced yield.
The bacterial promoter prepared by high-seed bacterial strains is combined with the embedded bacterial promoter. The bacterial promoter is directly applied to the roots of Platycodon through the degradable packaging body, and the rapid and efficient application is achieved using the symbiosis relationship of rhizobium and physical extrusion mechanism. , combined with oyster mushroom bran and corn flour, provides nutrients, regulates pH value and supplements of carbon, nitrogen and phosphorus, and inhibits harmful microorganisms.
The rapid and full application of bacterial promoters to the roots of Platycodon is achieved, which improves plant growth and resistance, reduces pests and diseases, enhances soil fertility, reduces waste of bacterial promoters, and improves yield and quality.
Smart Images
Figure CN119080563A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of growth-promoting bacteria agents, and specifically is a method for preparing a medicinal platycodon growth-promoting bacteria agent. Background Art
[0002] Platycodon grandiflorum, a plant of the genus Platycodon in the family Campanulaceae, is also known as the bundle flower and the bell flower. It is a perennial herb with a plant height of 30 to 100 cm. The whole plant has white latex, and the root is long and conical, fleshy and fleshy. The outer skin is yellow-brown or gray-brown. It grows in hillside grasslands, forest edges and other environments, and is slightly tolerant to semi-shade. Platycodon is a plant that can be used as medicine, food and appreciation. Its root is used as medicine. It tastes bitter and spicy, and is flat in nature. It has the functions of clearing the lungs, relieving sore throat, removing phlegm and draining pus. Modern pharmacological studies have shown that Platycodon also has anti-inflammatory, antipyretic, sedative, analgesic, hypoglycemic, hypolipidemic, cholesterol-lowering, inhibiting gastric acid secretion and anti-gastric ulcer effects. In recent years, the demand for Platycodon medicinal materials has continued to expand. However, Platycodon mainly comes from wild resources. Due to the predatory excavation of wild resources and the infertility of wild resources, wild resources are facing depletion and are far from meeting the needs. Research on Platycodon grandiflorum breeding technology is of great significance to the preservation of Platycodon grandiflorum germplasm resources, the protection of wild resources, and meeting the demand for Chinese medicinal materials.
[0003] In the process of cultivating and propagating Platycodon grandiflorum, if it is planted continuously on the same piece of land, even if the normal cultivation and management mode is adopted, there will be phenomena such as aggravation of diseases and insect pests, reduction of yield and decline of quality, which is called continuous cropping disorder. In order to alleviate the continuous cropping disorder of Platycodon grandiflorum, most of them will adopt intercropping and apply growth-promoting bacteria to promote the growth of Platycodon grandiflorum.
[0004] Among them, plant growth-promoting rhizobacteria (PGPR) are a type of beneficial fungi that live in or around the roots of plants and can promote plant growth and its absorption and utilization of mineral nutrients, and can inhibit harmful organisms. At present, many types of PGPR strains have been identified, and it has been found that the use of rhizosphere growth-promoting bacteria to develop growth-promoting agents for eliminating continuous cropping obstacles in the process of plant growth has great advantages and application potential. In the prior art, generally according to the characteristics of various strains, suitable strains are selected for cultivation of growth-promoting agents suitable for Platycodon grandiflorum, and then the growth-promoting agents are directly applied to the Platycodon grandiflorum planting site. When the growth-promoting agents need to be applied later, they can only be applied directly above the soil and the growth-promoting agents can only reach the Platycodon grandiflorum rhizosphere through slow infiltration to achieve a more sufficient growth-promoting effect. This method is inefficient and the application effect is not sufficient. Summary of the invention
[0005] In order to solve the above-mentioned problems of slow infiltration and high loss of subsequent application of growth-promoting bacteria, the purpose of the present invention is to provide a method for preparing a medicinal Platycodon growth-promoting bacteria agent, by combining the growth-promoting bacteria prepared from superior strains with the pre-buried growth-promoting bacteria, so that the growth-promoting bacteria can be naturally applied to the rhizosphere of Platycodon in the later stage as it grows, thereby achieving the effect of quickly, efficiently and fully applying the growth-promoting bacteria agent.
[0006] In order to achieve the above object, the technical scheme of the present invention is as follows: a method for preparing a medicinal platycodon growth-promoting bacterial agent, comprising the following preparation method:
[0007] Step 1: Bacteria culture: activate rhizobia or growth-promoting bacteria and inoculate them into the culture medium, then add sterile water and scrape off the bacterial moss with an inoculation needle, and vibrate and break it up with a vibrator to obtain a bacterial solution;
[0008] Step 2, raw material processing: crush the raw material, sieve it, stir and mix the raw material with distilled water, let it stand and take the supernatant;
[0009] Step 3, fermentation treatment: taking the bacterial strain and the supernatant into a fermentation bottle, shaking the bottle to mix, adjusting the pH value, and then fermenting to obtain a liquid growth-promoting bacterial agent;
[0010] Step 4, bacterial agent packaging: The growth-promoting bacterial agent is packaged in a manner of injecting a portion of the growth-promoting bacterial agent into a plurality of spherical packaging bodies, the packaging bodies being made of a degradable film, and the packaging bodies are sealed;
[0011] Step 5, applying the microbial agent: at the site where Platycodon grandiflorum is planted, take the liquid growth-promoting microbial agent not packaged in the packaging body and mix it evenly with the auxiliary matrix, and apply it directly to the roots of Platycodon grandiflorum. When covering the Platycodon grandiflorum with soil, cover the packaging bodies between adjacent Platycodon grandiflorums respectively, and glue the thorny plant branches to the top of the packaging body, and finally cover with soil, leaving one end of the branch protruding out of the soil.
[0012] Further, in step 1, the culture medium is K2HPO4·3H2O, 0.5 g; MgSO4·7H2O, 0.2 g; NaCl, 0.1 g; mannitol, 10 mL; yeast powder, 1.0 g; and distilled water is added to 1000 mL.
[0013] Further, in step 1, the culture temperature is 25-30°C, the culture time is 4-6 days, and the bacterial solution value is OD 600 .
[0014] Furthermore, in step 2, the raw materials are selected as oyster mushroom bran and corn flour, the weight ratio of oyster mushroom bran to corn flour is 5:1, and the oyster mushroom bran and corn flour are adjusted to a moisture content of 60%-70% by adding distilled water.
[0015] Furthermore, in step three, NaOH is added to adjust the pH value, and the pH value is adjusted to 6.5-7.5.
[0016] Furthermore, in step 4, the packaging bodies are divided into three sizes, with diameters of 18 to 23 cm, 15 to 18 cm, and 13 to 15 cm, respectively.
[0017] Furthermore, in step five, the auxiliary matrix is selected from soybean meal, starch, gelatin fish meal, glucose and castor, and the weight ratio of soybean meal, starch, gelatin fish meal, glucose and castor is 1:1:0.3:0.3:0.3.
[0018] Furthermore, in step five, a marking pen or marking tape is used to mark the protruding end of the branch, and the marking point is selected as the position where the branch is flush with the soil.
[0019] The principles and beneficial effects of the basic scheme are: 1. The present invention adopts a combination of strain optimization and microbial agent application method to achieve both strain and physical promotion of Platycodon grandiflorum growth, and the growth-promoting bacterial fertilizer is fully applied to the roots of Platycodon grandiflorum to achieve a sufficient growth-promoting effect of the growth-promoting bacterial agent.
[0020] 2. There is a symbiotic relationship between rhizobia and Platycodon grandiflorum. Rhizobia provide Platycodon grandiflorum with the necessary nutrients through the symbiotic interaction with Platycodon grandiflorum, promoting its growth and development. At the same time, Platycodon grandiflorum also provides a good living environment for rhizobia, allowing it to grow and reproduce better in the soil. This symbiotic relationship enables Platycodon grandiflorum to grow well in some environments where nitrogen sources are insufficient. At the same time, rhizobia can produce siderophores during growth. Siderophores are compounds produced by microorganisms that can bind to iron ions, and their main groups are derivatives of hydroxamic acid. The growth-promoting bacteria agent of the present invention can enable rhizobia to fully express siderophores during growth by adjusting the appropriate pH value and fully supplementing carbon, nitrogen and phosphorus in the culture medium. The growth-promoting bacteria agent is in an advantageous position when competing for iron with harmful microorganisms that produce less or even cannot produce iron carriers, so that the harmful microorganisms cannot obtain the required iron and are inhibited, thereby achieving the effect of improving plant nutrition;
[0021] And microbial iron carriers have rich functions. For plants, iron carriers affect plant health by promoting plant nutrient absorption, improving plant resistance, and inhibiting the growth of pathogens. In addition, for microorganisms, in addition to the function of iron ion absorption, they can also affect microbial biofilm formation, swimming ability, spore formation and other aspects.
[0022] 3. Both oyster mushroom bran and corn flour are rich in organic matter, which contain a large amount of nutrients such as nitrogen, phosphorus, potassium, etc., which can provide rich nutrients for the soil. When used in combination with rhizobia, the symbiotic nitrogen fixation of rhizobia can convert nitrogen in the air into nitrogen that can be used by plants, further enhancing the fertility of the soil and promoting plant growth. At the same time, because oyster mushroom bran and corn flour contain nutrients such as nitrogen, phosphorus, potassium, etc., they can provide a certain basis for the subsequent iron carriers for the growth of rhizobia, thereby achieving a more adequate growth-promoting effect of subsequent growth-promoting agents.
[0023] 4. When applying the microbial agent, firstly, applying the growth-promoting microbial agent just after sowing can provide a certain foundation for the growth of Platycodon grandiflorus. Then, during the growth process of Platycodon grandiflorus, the rhizomes of Platycodon grandiflorus will continue to become thicker and larger, and then will squeeze the soil around it. Therefore, the packaging body arranged between adjacent Platycodon grandiflorus will be squeezed by the adjacent rhizomes. In addition, the packaging body itself is made of degradable film. The degradable film is generally divided into the induced cracking stage, the cracking stage, the crushing stage and the film-free stage, and is slowly degraded from April to August. Therefore, the packaging body will break under its own material characteristics and the squeezing of the adjacent bases, and then the growth-promoting microbial agent in the packaging body will flow out. Because the packaging body is buried close to the rhizosphere where Platycodon grandiflorus grows, the growth-promoting microbial agent will flow to the root of Platycodon grandiflorus more fully and quickly to promote the growth of Platycodon grandiflorus.
[0024] Compared with the existing method of applying the growth-promoting bacteria above the soil in the later stage and allowing it to naturally settle downwards, the present method can make the growth-promoting bacteria flow to the rhizosphere more fully and quickly to achieve a better growth-promoting effect. In addition, by reducing the thickness of the sedimentation medium of the growth-promoting bacteria, it can also reduce the waste of the growth-promoting bacteria and reduce the problem that some growth-promoting bacteria fail to fully reach the roots of Platycodon grandiflorum and thus fail to achieve a full growth-promoting effect.
[0025] 5. When the dosage of growth-promoting bacteria in the package is reduced, the package will change its volume, so the soil around the package will be slightly displaced, thereby loosening the soil, and then realizing the gas circulation in the soil, providing a better growth environment for the microorganisms at the roots of Platycodon grandiflorum; at the same time, when the package is squeezed by the rhizome, it can be deformed upward or downward, and then the branches on the package will move vertically downward or upward. Through the vertical movement of the branches, the branches themselves or the thorns on the branches can be used to further loosen the soil and make the gas circulate fully. At the same time, after the branches move, the marked positions on the branches The position will produce corresponding changes. Later, by observing whether the marked position changes, it can be preliminarily judged whether the package in the soil is successfully broken and the growth-promoting bacteria are output. If it is observed that the branches of some Platycodon have not changed for a long time, it can be recorded to judge that the roots of the Platycodon may not have grown sufficiently, and the branches can be pressed hard by hand to artificially squeeze and break the package, thereby achieving the effect of supplementing the Platycodon with the growth-promoting bacteria. Even if there are some unchanged branches that have not been noticed by the staff, as the package itself degrades, the subsequent growth-promoting bacteria can automatically flow into the soil, and thus does not affect the nutritional supplement of Platycodon.
[0026] 6. The size of the packages is divided into three categories: large, medium and small. Packages of different sizes are buried in adjacent positions according to the quality of Platycodon grandiflorus seeds. Small, medium and large packages are used to bury superior, improved and inferior varieties, respectively. On the one hand, Platycodon grandiflorus seeds with better quality have sufficient conditions to grow larger rhizomes, so that smaller packages can be squeezed and crushed. In addition, because they have better conditions, the amount of growth-promoting bacteria added should be appropriate to reduce the possibility of root rot caused by excessive application. On the other hand, inferior seeds are the opposite of superior varieties. Larger packages can make them easier to contact and squeeze and crush. At the same time, because their seed quality is poor, more growth-promoting bacteria are needed to supplement their nutrition and achieve healthy growth. Therefore, by taking appropriate measures according to the different seed quality, both superior and inferior seeds can get sufficient growth promotion effects. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 This is a schematic diagram of the method for preparing the medicinal Platycodon grandiflorum growth-promoting bacteria agent in an embodiment of the present invention.
[0028] Figure 2 It is a schematic diagram of the application of the method for preparing the medicinal Platycodon grandiflorum growth-promoting bacteria agent in an embodiment of the present invention. DETAILED DESCRIPTION
[0029] The following is further described in detail through specific implementation methods:
[0030] Example 1
[0031] Basically as attached Figure 1 As shown: A method for preparing a medicinal platycodon growth-promoting bacterial agent, comprising the following preparation methods:
[0032] Step 1: Bacteria culture: activate rhizobia or growth-promoting bacteria and inoculate them into the culture medium, then add sterile water and scrape off the bacterial moss with an inoculation needle, and vibrate and break it up with a vibrator to obtain a bacterial solution;
[0033] The culture medium is K2HPO4·3H2O, 0.5g; MgSO4·7H2O, 0.2g; NaCl, 0.1g; mannitol, 10mL; yeast powder, 1.0g; distilled water added to 1000mL;
[0034] The culture temperature is 25-30°C, the culture time is 4-6 days, and the bacterial solution value is OD 600 .
[0035] Step 2, raw material processing: crush the raw material, sieve it, stir and mix the raw material with distilled water, let it stand and take the supernatant;
[0036] The raw materials are oyster mushroom bran and corn flour, the weight ratio of oyster mushroom bran to corn flour is 5:1, and the oyster mushroom bran and corn flour are adjusted to a water content of 60% to 70% by adding distilled water;
[0037] Step 3, fermentation treatment: taking the bacterial strain and the supernatant into a fermentation bottle, shaking the bottle to mix, adjusting the pH value, and then fermenting to obtain a liquid growth-promoting bacterial agent;
[0038] When adjusting the pH value, NaOH is added to adjust the pH value to 6.5-7.5;
[0039] Step 4, bacterial agent packaging: the growth-promoting bacterial agent is packaged, and the packaging method is to inject part of the growth-promoting bacterial agent into a plurality of spherical packaging bodies, the packaging bodies are made of a degradable film, and the packaging bodies are sealed;
[0040] The packaging body is divided into three sizes, with diameters of 18-23cm, 15-18cm and 13-15cm respectively;
[0041] Step 5, applying the microbial agent: at the site where Platycodon grandiflorum is planted, take the liquid growth-promoting microbial agent not packaged in the packaging body and mix it evenly with the auxiliary matrix, and apply it directly to the roots of Platycodon grandiflorum. When covering the Platycodon grandiflorum with soil, cover the packaging bodies between adjacent Platycodon grandiflorums respectively, and glue the thorny plant branches to the top of the packaging body, and finally cover with soil, leaving one end of the branch protruding out of the soil.
[0042] The auxiliary matrix is selected from soybean meal, starch, gelatin fish meal, glucose and castor, and the weight ratio of soybean meal, starch, gelatin fish meal, glucose and castor is 1:1:0.3:0.3:0.3;
[0043] Use a marker pen or marking tape to mark the end of the branch, and choose the marking point where the branch is flush with the soil.
[0044] The specific experimental process is as follows:
[0045] 1. Experimental Preparation
[0046] The test soil samples were from the soil of Platycodon grandiflorum planted for many years, collected from the Platycodon grandiflorum planting fields of farmers in Shahezi Town, Shangzhou District;
[0047] The experimental group was divided into an experimental group and a control group;
[0048] The experimental group used the raw materials and application method in Example 1 to prepare the growth-promoting bacteria agent and apply the growth-promoting bacteria agent;
[0049] Control group A, the growth-promoting bacteria agent was prepared using the raw materials in Example 1, but the application method was to apply it directly from above the soil later;
[0050] Control group B, no bacterial agent was applied, and the plants were planted directly.
[0051] 2. Experimental Results
[0052] Table 1 Effects of different groups on Platycodon grandiflorum growth
[0053] Group Whole plant length / cm Stem length / cm Root length / cm Whole plant fresh weight / g Stem fresh weight / g Root fresh weight / g Experimental Group 16.3091 10.6233 5.1033 0.2004 0.1578 0.0456 Control group A 14.0667 10.1233 4.3933 0.1539 0.1470 0.0184 Control group B 12.0918 8.6781 4.0979 0.1092 0.1021 0.0152 variance 4.452367843 1.020401013 0.26703172 0.00207963 0.000872523 0.00027904
[0054] Table 2 Effects of different groups on the growth rate of Platycodon grandiflorum
[0055] Group Root relative elongation / % Hypocotyl average value / mm Hypocotyl relative elongation / % Experimental Group 139.80 39.69 134.54 Control group 128.03 32.87 111.42 Control group B 118.78 30.99 108.35 variance 110.9893 20.95613333 204.9792333
[0056] By comparing Table 1 and Table 2, it can be found that the overall rhizome growth of the experimental group is better than that of the control group. Therefore, it can be concluded that the subsequent application of growth-promoting bacteria by pre-embedding can make the roots of Platycodon grandiflorum more fully contact with the growth-promoting bacteria and be absorbed, thereby achieving a more sufficient growth-promoting effect.
[0057] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0058] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.
Claims
1. A method for preparing a medicinal Platycodon grandiflorum growth-promoting agent, characterized in that: The invention comprises the following preparation methods: Step 1, bacterial culture: activate rhizobia or growth-promoting bacteria and inoculate them into the culture medium, then add sterile water and scrape off the bacterial moss with an inoculation needle, and vibrate and break it up with a vibrator to obtain bacterial liquid; Step 2, raw material processing: crush the raw material, sieve it, stir and mix the raw material with distilled water, let it stand and take the supernatant; Step 3, fermentation treatment: taking the bacterial strain and the supernatant into a fermentation bottle, shaking the bottle to mix, adjusting the pH value, and then fermenting to obtain a liquid growth-promoting bacterial agent; Step 4, bacterial agent packaging: the growth-promoting bacterial agent is packaged, and the packaging method is to inject part of the growth-promoting bacterial agent into a plurality of spherical packaging bodies, the packaging bodies are made of a degradable film, and the packaging bodies are sealed; Step 5, applying the microbial agent: at the site where Platycodon grandiflorum is planted, take the liquid growth-promoting microbial agent not packaged in the packaging body and mix it evenly with the auxiliary matrix, and apply it directly to the roots of Platycodon grandiflorum. When covering the Platycodon grandiflorum with soil, cover the packaging bodies between adjacent Platycodon grandiflorums respectively, and glue the thorny plant branches to the top of the packaging body, and finally cover with soil, leaving one end of the branch protruding out of the soil.
2. The method for preparing the medicinal Platycodon grandiflorum growth-promoting agent according to claim 1, characterized in that: In step 1, the culture medium is K2HPO4·3H2O, 0.5 g; MgSO4·7H2O, 0.2 g; NaCl, 0.1 g; mannitol, 10 mL; yeast powder, 1.0 g; and distilled water is added to 1000 mL.
3. The method for preparing the medicinal Platycodon grandiflorum growth-promoting agent according to claim 2, characterized in that: In step 1, the culture temperature is 25-30°C, the culture time is 4-6 days, and the bacterial solution value is OD 600 .
4. The method for preparing the medicinal Platycodon grandiflorum growth-promoting agent according to claim 3, characterized in that: In step 2, the raw materials are oyster mushroom bran and corn flour, the weight ratio of oyster mushroom bran to corn flour is 5:1, and the oyster mushroom bran and corn flour are adjusted to a water content of 60% to 70% by adding distilled water.
5. The method for preparing the medicinal Platycodon grandiflorum growth-promoting agent according to claim 4, characterized in that: In step 3, NaOH is added to adjust the pH value to 6.5-7.
5.
6. The method for preparing the medicinal Platycodon grandiflorum growth-promoting agent according to claim 5, characterized in that: In step 4, the packaging body is divided into three sizes, with diameters of 18 to 23 cm, 15 to 18 cm, and 13 to 15 cm respectively.
7. The method for preparing the medicinal Platycodon grandiflorum growth-promoting agent according to claim 6, characterized in that: In step five, the auxiliary matrix is selected from soybean meal, starch, gelatin fish meal, glucose and castor, and the weight ratio of soybean meal, starch, gelatin fish meal, glucose and castor is 1:1:0.3:0.3:0.
3.
8. The method for preparing the medicinal Platycodon grandiflorum growth-promoting agent according to claim 7, characterized in that: In step five, use a marker pen or marking tape to mark the protruding end of the branch, and choose the marking point where the branch is flush with the soil.