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Method for preventing soil-borne diseases of crops by using ammonium-reinforced biogas slurry

A technology for soil-borne diseases and crops, applied in the field of preventing and controlling soil-borne diseases of crops, can solve the problems of ineffective utilization, unreasonable use of water and fertilizer, and obstacles to continuous soil cropping, so as to reduce the incidence of soil-borne diseases and promote the recycling of resources. , the effect of shortened processing time

Inactive Publication Date: 2013-09-04
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is: on the one hand, due to the large-scale popularization of plastic greenhouses and solar greenhouses, the location of facility vegetable production is relatively fixed, the use of water and fertilizer is unreasonable, the cultivars are relatively single, and the phenomenon of soil continuous cropping obstacles is intensified; A large number of projects have been constructed, and the production of biogas slurry has continued to increase. Biogas slurry, as a nutrient-rich biomedical fertilizer, has shown good effects in preventing and controlling plant diseases in agricultural production, but it cannot be used efficiently. A method for flooding biogas slurry with ammonium sulfate added, a method for enhancing the effect of biogas slurry for preventing and controlling soil-borne diseases of crops

Method used

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  • Method for preventing soil-borne diseases of crops by using ammonium-reinforced biogas slurry
  • Method for preventing soil-borne diseases of crops by using ammonium-reinforced biogas slurry
  • Method for preventing soil-borne diseases of crops by using ammonium-reinforced biogas slurry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of Ammonium Enhanced Biogas Slurry

[0019] The raw material for fermentation is pig manure, collected from a pig farm near Jiangsu Academy of Agricultural Sciences. The inoculum sludge is the digested liquid of anaerobic fermentation of pig manure in our research laboratory. Under the condition of medium temperature [(37±2)℃], the anaerobic fermentation test was carried out in a continuous flow stirred reactor (CSTR). In the CSTR reactor Inoculate the sludge, and finally adjust with water to make the total solids content load 4.0%, pass nitrogen gas for 15 minutes to remove the air in the tank, and seal it. After fully fermenting for 28 days, the fermented liquid is released and introduced into a circular biogas slurry storage tank with a diameter of 3.5m and a depth of 1.2m. The main physical and chemical conditions of the original biogas slurry are shown in Table 1. According to the measured ammonium nitrogen concentration of the original biogas slurry,...

Embodiment 2

[0023] Inhibitory effect of ammonium-enhanced biogas slurry on the growth of Phytophthora capsici

[0024] The pathogenic fungus Phytophthora capsici was isolated from the rhizosphere soil of pepper root rot diseased plants, and was preserved by the Agricultural Waste Recycling Engineering Technology Research Center of the Institute of Environmental Protection, Jiangsu Academy of Agricultural Sciences. Phytophthora was inoculated into a PDA plate, cultured at 28-30° C. for 5 days, and then punched with a hole punch to obtain mycelial blocks with a diameter of 4 mm for use.

[0025] Preparation method of Phytophthora zoospore suspension: Phytophthora capsici was cultured in V8 medium for 5 days, and 20 hyphae blocks (Φ6 mm) from the edge of the colony were punched and put into a petri dish filled with 15 ml of sterile water. The side with mycelium was facing up, the liquid surface submerged the mycelium block, and the light and dark were alternately cultured (12 h / 12 ​​h) for 2...

Embodiment 3

[0031] Inhibitory effect of biogas slurry on plate growth of two pathogens of strawberry.

[0032] Test method: plate mycelial growth inhibition method: mix 5ml of ammonium-enhanced biogas slurry or sterile water or fresh biogas slurry with 45ml of dissolved PDA medium at 50-60°C and pour it on the plate, and inoculate a diameter of 1 in the center of the plate after solidification 6 mm of pathogenic fungus hyphae, cultured at 28°C, measured the colony diameter of Solitaire blight after 72 hours, and measured the colony diameter of Fusarium wilt after 120 hours, with 5 replicates for each treatment.

[0033] The test group was ammonium-enhanced biogas slurry, that is, mixed with 5ml of ammonium-enhanced biogas slurry and dissolved 50-60°C PDA medium and poured on the plate; control 1 was a blank control, that is, mixed with 5ml sterile water and dissolved 50-60°C PDA medium PDA medium was mixed and poured on the plate; control 2 was mixed with 5ml of fresh biogas slurry and di...

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Abstract

The invention relates to a method for preventing soil-borne diseases of crops by using ammonium-reinforced biogas slurry. The method comprises the specific steps as follows: a leisure period of a greenhouse after planting, harvest and cleaning is selected for turn over the soil; ridging is performed in the greenhouse along the periphery, the height of the ridge ranges from 15 cm to 20 cm, and the width ranges from 15 cm to 20 cm; anaerobic fermentation raw materials for biogas engineering are introduced into an anaerobic fermentation tank for fermentation, discharged fresh biogas slurry which is fermented for more than 25 days is taken to be injected into a biogas slurry storage pool and stirred to a suspension state, during stirring, ammonium sulfate is added to dissolve the slurry completely, and the concentration of ammonium nitrogen in the biogas slurry is regulated to 1000 mg / L; the biogas slurry in the biogas slurry storage pool is pumped out and introduced into the greenhouse for flood irrigation, at least 10 cm biogas slurry is kept on the surface of the soil layer within the flooding period, when the liquid level drops due to evaporation or infiltration, the biogas slurry is introduced repeatedly until the flooding period is over, and then natural ventilation or air-cure is performed; and when the moisture content of the soil reaches 15%-25%, the ridge is tilled, and the soil is turned over for ridging combined with a base fertilizer.

Description

technical field [0001] The invention relates to a method for using ammonium to strengthen biogas slurry to prevent and control crop soil-borne diseases. It belongs to the field of comprehensive utilization of agricultural waste. Background technique [0002] With the adjustment of the rural economic structure, the status and role of protected cultivation in rural economic development has become more and more prominent. However, due to long-term fertile water, lack of leaching in the soil, and single types of crops, the nutrients in the soil are unbalanced, and the soil quality deteriorates sharply, such as soil acidification, salinization, soil pathogenic bacteria proliferation, and soil microbial flora imbalance. Continuous cropping obstacles such as aggravation of soil-borne diseases. Obvious obstacles to continuous cropping appear after 3-4 stubbles are planted in protected areas in many areas. Jiangsu Academy of Agricultural Sciences surveyed and isolated vegetable pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N59/02A01P3/00C05G1/00
Inventor 曹云常志州马艳
Owner JIANGSU ACAD OF AGRI SCI
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