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Bacillus amyliquefaciens strain and application thereof

A technology for dissolving amyloid spores and Bacillus megaterium, applied in the field of microorganisms, can solve problems such as huge costs, and achieve the effects of reducing usage, protecting the ecological environment, and improving crop stress resistance

Inactive Publication Date: 2016-08-03
武汉合缘绿色生物股份有限公司
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And the cost is huge

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  • Bacillus amyliquefaciens strain and application thereof
  • Bacillus amyliquefaciens strain and application thereof

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Embodiment Construction

[0017] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0018] The screening process of HY-05 bacterial strain comprises the following steps:

[0019] 1) Strain purification: prepare LB (Luria-Bertani) medium: add peptone 10g, yeast extract 5g, NaC110g to 950ml distilled water, adjust the pH value to 7.0, and dilute to 1000ml;

[0020] Potato medium (PDA medium): 200g potato, 20g sucrose, 15-20g agar, 1000ml water, natural pH; cut the peeled potato into pieces and boil for half an hour, filter with gauze, add sucrose and aga...

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Abstract

The invention discloses a bacillus amyliquefaciens strain HY-05 with classification and nomenclature of bacillus megaterium (bacillus amyliquefaciens), which is sent to China Center for Type Culture Collection in Wuhan with the accession number of CCTCCM2015697 at November 20, 2015. HY-05 bacterial strain has good biological prevention and control function for pathogenic bacteria in continuous cropping land and the land with serious disease. The continuous breeding of HY-05 bacterial strain can inhibit the breeding of pathogenic bacteria, so that the soil microbial community structure is improved, the stress resistance of crop is improved, the crop yield is increased, the application amount of chemical agent is reduced, the ecological environment is protected, and the agriculture continuable development is promoted. The bacterial strain has good promotional value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus amyloliquefaciens and its application in preventing and treating soil-borne diseases. Background technique [0002] With the adjustment of the agricultural industry structure, facility agriculture mainly based on greenhouses and greenhouses has developed rapidly. Due to various reasons, crop repetition is becoming more and more serious. Soil-borne diseases are a general term for plant diseases transmitted by soil as a medium. They refer to pathogens living in the soil, such as fungi, bacteria, nematodes and viruses, which attack crops from the roots or stems of crops when conditions are suitable. caused diseases. There are many kinds of soil-borne diseases in China. Common soil-borne diseases include blight, fusarium wilt, verticillium wilt, soft rot, root rot, bacterial wilt, and sclerotinia. Rotten or stem rot dampens, seedlings will so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/02A01P3/00C12R1/07
CPCC12Q1/02C12N1/205C12R2001/07
Inventor 徐维烈杨涛杨国平熊峰徐辉袁庆丰
Owner 武汉合缘绿色生物股份有限公司
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