Screening method of endophytic biocontrol bacterial strain of banana wilt

A technology of banana fusarium wilt and a screening method is applied in the field of screening of banana fusarium wilt biocontrol strains, and can solve the problems of abandoned banana orchards, frequent damage of banana fusarium wilt, lack of high-yield and disease-resistant varieties, and the like

Inactive Publication Date: 2016-12-14
MICROBIOLOGY RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the large-scale continuous cropping of bananas has caused frequent damage to the soil-borne fungal disease Fusarium oxysporum. % or more, leading to the abandonment of banana orchards and almost no harvest
At present, due to the lack of effective high-yield and disease-resistant varieties, the control of banana fusarium wilt mostly relies on chemical control and agricultural control, but none of them can achieve the desired control effect

Method used

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  • Screening method of endophytic biocontrol bacterial strain of banana wilt
  • Screening method of endophytic biocontrol bacterial strain of banana wilt
  • Screening method of endophytic biocontrol bacterial strain of banana wilt

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Activation and cultivation of step 1. bacterial strain Activation of endophytic fungal strains for testing on CMMY plate medium (malt juice agar 10g / L, corn meal agar 8.5g / L, yeast extract 2g / L, agar 7.5g / L) , 28 ° C incubator dark culture 10d. Cut the activated endophyte mycelium block and inoculate it on the oat plate medium (oat flour 10g / L, agar 18g / L, MgSO 4 ·7H 2 O1g / L, KH 2 PO 4 1.5g / L,NANO 3 1g / L), each plate was inoculated with 3 bacterial blocks, cultured for 10 days, and set aside.

[0029] Step 2. Activation and cultivation of pathogenic bacteria. Banana Fusarium wilt pathogen-Fusarium is activated on a PDA plate medium (potatoes 200g / L, boiled for 30mins, filtered 4 times to get the filtrate; glucose 15g / L; agar 15g / L), at 28°C Cultured in the dark in the incubator for 7 days. Cut the activated Fusarium mycelium block and inoculate it on the water agar plate medium (agar 9g / L), inoculate 3 bacterial blocks on each plate, cultivate for 10 days, and s...

Embodiment 2

[0035] Embodiment 2 endophytic fungus-banana seedling symbiont establishment

[0036] The experimental results show that the endophytic fungus strains can stably colonize the host plant without pathogenicity and form a mutualistic symbiont with the host plant. After the endophytic fungus-banana seedling symbiont is established, the banana seedling plants can still be healthy growth, and the microscopic examination of the root tissue found that there was endophytic fungal mycelium in the root tissue (such as figure 2 shown).

Embodiment 3

[0037] Indoor plate screening of endogenous biocontrol bacterial strains of embodiment 3 resistance to banana fusarium wilt

[0038] Test material

[0039] Banana tissue culture seedlings for testing: Guijiao No. 6 provided by Guangxi Plant Tissue Culture Seedling Co., Ltd.

[0040] Tested endophytic fungal strains: 8 endophytic fungal strains were isolated from sugarcane rhizosphere and mangrove plants and kept in the laboratory.

[0041] The pathogenic bacteria to be tested: Fusarium wilt pathogen No. 4 physiological race, isolated from Hainan banana wilt disease strain, preserved in the laboratory.

[0042]According to the method of the present invention, the endophyte fungal strains isolated in the laboratory are screened for biocontrol function. the result shows, image 3 and Figure 4 As shown, different endophytic fungal strains have different biocontrol effects on banana Fusarium wilt. The indoor plate biocontrol effect of strain L-14 is the best, up to 72.42%, whi...

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Abstract

The invention provides a screening method of an endophytic biocontrol bacterial strain of banana wilt. The screening method includes the steps of: (A) activating and cultivating the bacterial strain; (B) activating and cultivating pathogenic bacteria; (C) co-cultivating the endophytic bacterial strain and banana tissue culturing seedlings; and (D) moving the endophytic fungus-banana seedling symbiont along with the culture medium onto a water-agar plate culture medium on which fusarium grows, and culturing the symbiont in an incubator. The plate screening method of the endophytic biocontrol bacterial strain resisting the banana wilt can effectively screen the endophytic fungus bacterial strain that has excellent biocontrol function against the banana wilt, thereby providing theoretical fundament for establishment of biocontrol technologies of the banana wilt.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a screening method for biocontrol strains of banana fusarium wilt. Background technique [0002] Banana is an important economic crop in the subtropical regions of southern my country. However, in recent years, the large-scale continuous cropping of bananas has caused frequent damage to the soil-borne fungal disease Fusarium oxysporum. More than %, resulting in abandoned banana orchards, almost no harvest. At present, due to the lack of effective high-yield and disease-resistant varieties, the control of banana fusarium wilt mostly relies on chemical control and agricultural control, but none of them can achieve the desired control effect. As one of the measures to comprehensively control banana wilt, biological control is of great significance to the development of ecological balance and sustainable agriculture. [0003] Plant endophytic fungi are a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12Q1/04C12R1/77
CPCC12Q1/18C12Q1/04
Inventor 谢玲蓝桃菊张雯龙农倩苏琴张艳覃丽萍陈艳露
Owner MICROBIOLOGY RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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