Composite microbial agent and preparation method and application thereof

A technology of compound microbial agents and strains, which is applied in the field of microorganisms, can solve the problems of soil disinfection microbial community destruction, high development costs of crops resistant to diseases and insect pests, and damage to the balance of soil ecological structure, so as to improve crop resistance to stress and enhance crop resistance to diseases and insect pests ability to avoid damage

Inactive Publication Date: 2020-10-30
陕西均良土壤环境技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, chemical pesticides may cause additional pollution; the development of crops resistant to diseases and insect pests is costly; crop rotation is cumbersome and time-consuming; soil disinfection will also destroy beneficial microbial communities and disrupt the balance of soil ecological structure

Method used

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  • Composite microbial agent and preparation method and application thereof
  • Composite microbial agent and preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0043] (4) Preparation method of bacterial agent

[0044] 1) 8 strains were activated in NA solid slant medium respectively,

[0045] 2) The activated strains were respectively inoculated into sterilized LB liquid medium, and cultured in a constant temperature incubator at 37° C. for 24 hours.

[0046] 3) Adjust the bacterial content of 8 strains to be greater than or equal to 4×10 9 CFU / mL, and then mix the 8 kinds of bacterial solutions according to the volume ratio (1:1:1:3:1:1:3:1) to obtain a liquid composite microbial bacterial agent.

[0047] 4) The sterilized and dried zeolite is quantified and used as an attachment carrier for later use.

[0048] 5) The liquid compound microbial bacterial agent is mixed with the zeolite at a ratio of 0.5 kg of zeolite per liter of the mixed bacterial solution.

[0049] 6) Dry the above mixture in a dark and ventilated place to complete the preparation of the composite microbial agent.

[0050]The application of a compound microbia...

Embodiment 1

[0053] A kind of preparation method of composite microbial bacterial agent of the embodiment of the present invention, comprises

[0054] (1) Activate 8 kinds of strains in sterilized NA solid medium. The formula of NA solid medium is tryptone 10g, yeast extract powder 5g, sodium chloride 10g, deionized water 1000mL, agar 15-20g, pH 7.0.

[0055] (2) The activated 8 strains were inoculated into LB liquid medium respectively, and cultured in a constant temperature incubator at 37° C. for 24 hours. The formula of LB liquid medium is tryptone 10g, yeast extract powder 5g, sodium chloride 10g, deionized water 1000mL, pH7.0.

[0056] (3) Adjust the cultured bacterial solution so that the bacterial content of each strain is greater than or equal to 4 × 10 9 CFU / mL, and then mix the 8 kinds of bacterial solutions according to the volume ratio (1:1:1:3:1:1:3:1) to obtain a liquid composite microbial bacterial agent.

[0057] (4) The liquid compound microbial bacterial agent is mixe...

Embodiment 2

[0059] The application of a kind of composite microbial bacterial agent of the embodiment of the present invention comprises the following steps:

[0060] The experimental site is located in the greenhouse of Shenmu Mu Us Desert Control and Afforestation Base (more than 4 years of continuous cropping tomato), and four treatments are established: the control group: the original continuous cropping soil, without adding other substances; Mix with continuous cropping soil; treatment group 2: mix bio-organic fertilizer and zeolite as base fertilizer and apply to continuous cropping soil, and the amount of zeolite is 20kg per mu; treatment group 3: mix bio-organic fertilizer and compound bacterial agent of the present invention as base fertilizer and apply For continuous cropping soil, the dosage of bacterial agent is 20kg per mu. Four groups of experiments, tomato plant spacing 40cm, row spacing 50cm, all kinds of management measures are the same, the number of diseased and wilted ...

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Abstract

The invention discloses a composite microbial agent and a preparation method and application thereof. The composite microbial agent is prepared by uniformly mixing a cellulose decomposition strain, alignin decomposition strain, protein and a starch degradation strain, wherein the cellulose decomposition strain is one or more of ginseng bacillus, bacillus megatherium and bacillus aryabhattai, thelignin decomposition strain is Klebsiella, and the protein and starch degradation strains are one or more of bacillus thuringiensis, lysine borate bacillus, bacillus licheniformis and bacillus acidophilus. According to the invention, the microbial agent is selected to prevent and treat greenhouse vegetable continuous cropping obstacles, and related functional microorganisms are utilized to condition the nutritional status and microbial structure of the continuous cropping soil, so that the stress resistance of crops is improved, and the purpose of eliminating the continuous cropping obstaclescan be achieved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to the field of compound microbial bacterial agents for eliminating obstacles to continuous cropping of vegetables in greenhouses, and particularly relates to a compound microbial bacterial agent and its preparation method and application. Background technique [0002] In the cultivation of vegetables in greenhouses, due to the pursuit of high yield, long-term continuous cropping and abuse of chemical fertilizers and pesticides lead to deterioration of soil quality, which also leads to the problem of continuous cropping obstacles. Continuous cropping obstacle refers to the situation that the same species or crops with close relatives are planted on the same land one after another, resulting in abnormal growth and development of crops. Continuous cropping obstacles often lead to crop yield and quality decline, low seedling emergence rate or even no seedlings or death. Continuous cr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/23A01N63/20C09K17/14A01P21/00A01P3/00A01P1/00C12R1/07C12R1/11C12R1/22C12R1/10C09K101/00
CPCC12N1/20A01N63/22A01N63/23A01N63/20C09K17/14C09K2101/00
Inventor 李宇刘永军张至张驰白慧
Owner 陕西均良土壤环境技术有限责任公司
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