Polysporus trichoderma and application thereof

A technology of Trichoderma polyspores and spores, applied in the field of Trichoderma polyspores and its application

Active Publication Date: 2015-05-20
四川臻润农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many types of antagonistic Trichoderma, including Trichoderma hamayum, Trichoderma viride, Trichoderma longibranchiatum, Trichoderma harzianum, and Trichoderma harzianum Gliocladium virens of the genus, and the use of Trichoderma polysporum to control plant fungal diseases has not been reported.

Method used

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  • Polysporus trichoderma and application thereof
  • Polysporus trichoderma and application thereof
  • Polysporus trichoderma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Trichoderma polyspora ( Hypocrea pachybasioides ) Isolation and preservation of FSR-97 strain

[0047] The strain was isolated from the rhizosphere soil of the perennial ginseng plant in the elm ginseng cultivation field in Fusong County, Jilin Province. The above soil samples were collected and passed through a 2 mm sieve after removing litter on the surface. Weigh 10 g of fresh soil samples, put them into a conical flask filled with glass beads and 90 mL of sterile saline, shake fully for 30 min, and mix the samples with sterile water evenly to obtain a soil suspension. Take 1 mL of shaking liquid under sterile conditions, add 9 mL of sterile normal saline, and make 10 -2 、10 -3 、10 -4 diluent. 100 μL of each dilution was drawn and added to the potato dextrose agar medium (PDA) plate, uniformly spread by the plate dilution method, each treatment was repeated 3 times, and cultured in a 25 °C incubator for 7 days. After selecting a single colony an...

Embodiment 2

[0051] Example 2 Inhibitory effect of Trichoderma multispora FSR-97 strain on 7 kinds of pathogenic bacteria causing ginseng fungal diseases

[0052] The inhibitory effect of strain FSR-97 on 7 kinds of pathogenic bacteria of ginseng was determined by 3-point cup-and-saucer confrontation and growth rate method: the activated ginseng pathogenic bacteria colony was made into a cake of corresponding size with a puncher with a diameter of 5 mm, and sterile inoculated To the center of the potato dextrose agar (PDA) plate (diameter 90 mm), insert a bacterial cake of the bacteria to be tested (FSR-97) on each of the three sides 2.5 cm away from the pathogenic bacteria, and the other side is blank. As the treatment group, each treatment was repeated 3 times; in addition, only ginseng pathogenic bacteria were inoculated on a PDA plate. Diameter (unit: mm), and calculate the bacteriostatic rate according to the following formula. Each pathogen was repeated 3 times, and the results were...

Embodiment 3

[0057] Embodiment 3 Trichoderma multispora FSR-97 culture fluid, the preparation of spore suspension and fermented liquid

[0058] Activate the slant of Trichoderma multispora FSR-97 test tube, take two 5 mm bacterial cakes and inoculate them in 100 mL potato dextrose liquid medium (PDB) in a 250 mL Erlenmeyer bottle, and cultivate them at 170 r / min, 25 °C After 48 h, the seed solution was obtained; the seed solution of Trichoderma multispora FSR-97 was inoculated in the fermentation medium at 10% (volume ratio), and cultured at 170 r / min, 25 °C for 96 hours to obtain the culture solution; Trichoderma multispora FSR-97 culture solution was filtered through two layers of sterile gauze, the filtrate was counted by a hemocytometer, and the spore suspension was dispersed to 6 g L -1 Sodium carboxymethylcellulose (CMC) solution to obtain a spore suspension with a spore content of 3 × 10 5 cfu mL -1 ; Centrifuge the Trichoderma multispora FSR-97 culture broth at 8000 r / mim fo...

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Abstract

The invention discloses a polysporus trichoderma (Hypocrea pachybasioide) FSR-97 strain, wherein the preservation unit is China General Microbiological Culture Collection Center, the preservation date is 19, September, 2014 and the preservation number is CGMCC No.9691. The strain is cultured on a potato dextrose agar culture medium (PDA) plate at 25 DEG C and rapidly grows, and a monospore is germinated to form a white bacterial colony; the colour on the back surface of the bacterial colony is transited from colourless to yellow. The polysporus trichoderma (Hypocrea pachybasioide) FSR-97 has an effective broad-spectrum antibacterial effect on the main pathogenic bacteria causing the panaxginsen fusarium rot, epidemic disease, sclerotiniose, root rust rot, black spot, rhizoctonia solani, and botrytis. The invention further discloses a plurality of biocontrol mechanisms of the polysporus trichoderma (Hypocrea pachybasioide) FSR-97 for preventing and controlling the plant fungous disease, and an application for preparing a microbial preparation to prevent and control the plant fungous disease.

Description

technical field [0001] The invention relates to a strain of Trichoderma multispora and its application. Background technique [0002] Ginseng (Panax ginseng C.A. Mey) is a perennial root-negative dicotyledonous medicinal plant of Araliaceae Panax. It is a precious medicinal material in my country and has the reputation of "the king of herbs". Ginseng is widely planted in China, South Korea, North Korea, Russia and other countries. Among them, Northeast China, especially Jilin Province, is the main production area of ​​ginseng and has become one of the important pillar industries there. The long-term artificial planting and variety selection of ginseng is difficult, which leads to the degradation of ginseng germplasm, serious diseases, poor quality and low yield. Extensive use of chemical pesticides leads to pesticide residues and environmental pollution, which reduces the safety and commodity value of ginseng medicinal materials. Disease prevention and control has become o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 杨利民肖春萍韩梅
Owner 四川臻润农业科技有限公司
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