9 results about "Fermentative hydrogen production" patented technology

The application discloses a method for improving hydrogen production performance of Clostridium butyricum by using hematite nanoparticles, and belongs to the technical field of biological hydrogen production and microbial metabolism regulation. The method improves the hydrogen production performance by adding hematite nanoparticles into an anaerobic fermentation hydrogen production system of Clostridium butyricum for anaerobic fermentation. The application first discloses a specific double regulation mechanism of hematite nanoparticles on hydrogen production of Clostridium butyricum. By using hematite nanoparticles as a core regulation material, and through precise concentration gradient addition and stage temperature synergistic regulation, the hydrogen production lag period can be greatly shortened, the initial hydrogen production rate can be improved, byproduct synthesis of butyric acid can be inhibited, and the efficient hydrogen production path of acetic acid can be strengthened. The core technical problems that existing iron-based additives cannot shorten the hydrogen production lag period, the initial hydrogen production efficiency is low, and product feedback inhibition is easily caused are solved. The method is simple to operate, has strong industrialization adaptability, and can be widely applied to industrialization batch and continuous production of dark fermentation biological hydrogen production.

The invention relates to a method for producing hydrogen alkane through two-stage anaerobic fermentation of lincomycin fungus residues, and belongs to the technical field of biochemistry. The invention aims to improve the bioenergy conversion efficiency of the antibiotic mushroom dregs, reduce the harm caused by residues of produced antibiotics and reduce the cost of innocent treatment of the antibiotic mushroom dregs. The invention provides a method for producing hydrogen and methane in two stages by utilizing lincomycin fungus residues. The method comprises the following steps: carrying out hydrothermal pretreatment on the lincomycin fungus residues, and taking supernate; selecting an activated fermentation liquid as an inoculum, inoculating the inoculum into the supernatant, and carrying out first-stage anaerobic fermentation to obtain hydrogen; biogas slurry of an anaerobic fermentation methane production system or sludge of an anaerobic fermentation link in the antibiotic wastewater sewage treatment process is selected as seed sludge to be inoculated into residues at the hydrogen production fermentation tail end, and second-stage anaerobic fermentation is performed to obtain methane. Development of a novel efficient pretreatment technology and a fermentation mode becomes a critical path for breaking through the bottleneck of the current technology, and a high-quality substrate is provided for subsequent anaerobic fermentation.

The application belongs to the technical field of biological fermentation, and particularly relates to a dark fermentation mixed flora, a culture method thereof and application of the dark fermentation mixed flora in hydrogen production from waste oil reservoirs. In the application, a mixed flora capable of producing hydrogen by using waste oil reservoirs is disclosed, the mixed flora comprising Thermotoga hypogea, Thermotoga elfii, Thermus sp., Propionibacterium freudenreichii, Butyrivibrio fibrisolvens and Pseudomonas aeruginosa. The mixed flora can not only produce hydrogen by using crude oil as raw material, but also improve the flowability of the oil layer, improve the interface between the oil layer and the water layer, and crack heavy components in the crude oil, so that the oil layer can be fully utilized for fermentation hydrogen production, and the efficiency of hydrogen production by microorganisms using waste oil reservoirs is improved.

The application discloses a method for producing hydrogen through Clostridium pasteurianum-Rhodobacter sphaeroides coupling fermentation based on nitrogen limitation and application thereof. In the method, activated Clostridium pasteurianum is subjected to dark fermentation for producing hydrogen in a nitrogen-free culture medium and in an argon environment; then, the pH value of the obtained culture solution is adjusted to 7.0-8.0, the activated Rhodobacter sphaeroides is inoculated after selective sterilization, and the light fermentation for producing hydrogen is carried out in the argon environment. In the application, the fermentation solution of Clostridium pasteurianum does not need to be subjected to centrifugal removal of bacterial bodies and the like, and only needs to be simply adjusted in pH value, so that the light fermentation for producing hydrogen of Rhodobacter sphaeroides can be directly carried out. In the application, the nitrogen limitation is used to inhibit excessive proliferation of bacterial bodies, save carbon source flow, induce high expression of nitrogen fixation enzyme, and open a second high-efficiency hydrogen production path in addition to hydrogenase, so that the hydrogen production yield is further improved while the cost of nitrogen source is reduced. The hydrogen production yield of the application reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The invention discloses a method for enhancing hydrogen production of anaerobacter thermosaccharolyticus by using polyaluminum chloride, and belongs to the technical field of hydrogen production by microbial fermentation. A method for enhancing hydrogen production of anaerobacter thermosaccharolyticus by using polyaluminum chloride comprises the following steps: S1, preparation of a seed solution: inoculating a strain into a seed culture medium for activation and amplification culture to obtain the seed solution; and S2, fermentation hydrogen production: inoculating the seed solution into a fermentation culture medium containing polyaluminum chloride, and carrying out anaerobic fermentation to obtain hydrogen. According to the method, the high-performance hydrogen production strain is combined with a polyaluminum chloride strengthening strategy, so that a considerable hydrogen production effect is obtained, and important technical support and reference value are provided for resource utilization of biomass wastes and industrial production of clean hydrogen energy.

This invention provides a method for hydrogen production from organic wastewater through dark fermentation, belonging to the field of fermentation hydrogen production technology. The method includes: mixing organic wastewater, biochar loaded with metal nanoparticles, and a domesticated butyric acid-producing mixed bacterial community; performing micro-electrolysis and bioactivation treatment to obtain a pretreated solution; then subjecting the pretreated solution to hydrogen-producing phase fermentation treatment, adding an electron mediator to obtain a fermentation broth containing volatile acids; further, subjecting the fermentation broth to de-inhibition phase treatment, using adsorbent materials to adsorb volatile acids, and simultaneously converting some of the adsorbed volatile acids into butyric acid through acid-producing bacteria to obtain a post-fermentation solution; electrolyzing the post-fermentation solution, utilizing electroactive hydrogen-producing bacteria for extracellular electron transfer to convert residual organic matter in the solution into hydrogen gas to obtain crude hydrogen, which is then purified to obtain hydrogen gas. This invention solves the problems of low utilization rate of organic wastewater, inhibition of hydrogen production by volatile acids, and the impact of environmental parameter fluctuations on yield in existing processes.

The present application relates to a kind of high-efficiency denitrification method based on anaerobic hydrogen production coupling hydrogen-type autotrophic denitrification, it includes S1: using the high concentration organic wastewater of COD concentration ≥5000mg / L, by mechanical crushing and homogenization processing ensure that particle size ≤1mm, ultrasonic treatment is strengthened organic matter dissolution;S2: first to wastewater inoculation acid-producing bacteria, at 34-38 ℃ continuous stirring fermentation is carried out, so that acid-producing bacteria is converted into volatile fatty acid VFA into complex organic matter, VFA concentration reaches 2000-3000mg / L;Again, adjust pH to 5.5-6.5, inoculation hydrogen-producing bacteria and carry out fermentation hydrogen production;S3: tail gas generated in fermentation process is absorbed after alkali liquor, obtain purified hydrogen, collection;S4: in upflow packed bed reactor, load packing and inoculate hydrogen autotrophic denitrifying bacteria, hydrogen is injected into reactor by hollow fiber membrane gas distribution device, so that bubble diameter ≤50 μm, control water temperature 30-35 ℃, HRT is 4-12h.The present application obtains hydrogen by organic waste fermentation for denitrification system, and improves hydrogen utilization, saves energy consumption, realizes the green recycling goal of waste treatment.

The application provides a composite microbial inoculum for hydrogen production by fermentation and application thereof. The composite microbial inoculum comprises Priestia aryabhattai YLS2, Niallia nealsonii YLS4 and Lysinibacillus boronitolerans S5; wherein the preservation number of the Priestia aryabhattai YLS2 is CGMCC NO.32138, the preservation number of the Niallia nealsonii YLS4 is CGMCC NO.32137, and the preservation number of the Lysinibacillus boronitolerans S5 is CGMCC NO.32139. The hydrogen production process is optimized by the synergistic effect of multiple microorganisms, the composite microbial inoculum for hydrogen production by fermentation can improve the hydrogen production and the stability in the gas production process, and has significant environmental benefits and economic potential.