21 results about "Fermentative hydrogen production" patented technology

The invention belongs to the technical field of biological fermentation hydrogen production. The invention provides a method for producing hydrogen by dark fermentation of pericarp biomass, which comprises the following steps: drying pericarp, crushing, and sieving to obtain powdery pericarp particles; performing acid heat pretreatment on the powdery fruit peel particles to obtain fruit peel pretreatment liquid; and inoculating the clostridium butyricum solution into the pericarp pretreatment liquid, and carrying out dark fermentation under an anaerobic condition to obtain a fermentation gas containing hydrogen. According to the method, the pericarp byproducts are used for dark fermentation for hydrogen production, so that high-value recycling utilization of agricultural wastes is realized; lignin in the pericarp is effectively removed through acid heat pretreatment, the availability of cellulose and hemicellulose is remarkably improved, conditions such as acid concentration, temperature and time are optimized, the inhibition effect of by-products is avoided, and the hydrogen production efficiency is remarkably improved; moreover, the method has the advantages of good stability and high repeatability, and provides a new technical approach for efficient production of clean energy.

The invention discloses a domestication method for efficiently degrading tobacco waste to produce hydrogen by using mixed flora, which comprises the following steps: carrying out anaerobic enrichment domestication culture by taking manure compost as a bacterial source and cellulose prepared from tobacco waste as a core component of a domestication culture medium; the domestication culture medium contains cellulose, ammonium bicarbonate and monopotassium phosphate, and the cellulose concentration gradient is gradually increased without adjusting the pH value; stopping culturing when the hydrogen content in the biogas is reduced by 10%, transferring the biogas to a fresh domestication culture medium for passage according to an inoculum size of 10-20%, and continuously domesticating for 20-40 rounds; inoculating the domesticated mixed flora culture solution into a cellulose liquid fermentation culture medium containing microcrystalline cellulose, ammonium bicarbonate, monopotassium phosphate and a nutrient solution according to the inoculum size of 10-20%; the initial pH of the fermentation liquor is adjusted to 6.5-7.5, high-purity nitrogen is introduced to create an anaerobic environment, and hydrogen is produced through fermentation under the conditions of 35 + / -1 DEG C and 120 r / min. According to the method, flora is easy to culture, hydrogen production is efficient and stable, tobacco waste resources can be utilized, flora stability is high, adaptability is high, and strict sterile conditions are not needed.

The application discloses a method for improving hydrogen production performance of Clostridium butyricum by using hematite nanoparticles, and belongs to the technical field of biological hydrogen production and microbial metabolism regulation. The method improves the hydrogen production performance by adding hematite nanoparticles into an anaerobic fermentation hydrogen production system of Clostridium butyricum for anaerobic fermentation. The application first discloses a specific double regulation mechanism of hematite nanoparticles on hydrogen production of Clostridium butyricum. By using hematite nanoparticles as a core regulation material, and through precise concentration gradient addition and stage temperature synergistic regulation, the hydrogen production lag period can be greatly shortened, the initial hydrogen production rate can be improved, byproduct synthesis of butyric acid can be inhibited, and the efficient hydrogen production path of acetic acid can be strengthened. The core technical problems that existing iron-based additives cannot shorten the hydrogen production lag period, the initial hydrogen production efficiency is low, and product feedback inhibition is easily caused are solved. The method is simple to operate, has strong industrialization adaptability, and can be widely applied to industrialization batch and continuous production of dark fermentation biological hydrogen production.

The invention relates to a high-efficiency nitrogen removal method based on coupling of anaerobic hydrogen production and hydrogen type autotrophic denitrification, which comprises the following steps: S1, using high-concentration organic wastewater with the COD (Chemical Oxygen Demand) concentration of more than or equal to 5000mg / L, carrying out mechanical crushing and homogenization treatment to ensure that the particle size of particles is less than or equal to 1mm, and carrying out ultrasonic treatment to enhance the dissolution of organic matters; s2, firstly inoculating acid-producing bacteria into the wastewater, and carrying out continuous stirring fermentation at 34-38 DEG C, so that the acid-producing bacteria convert complex organic matters into volatile fatty acid (VFA), and the concentration of the VFA reaches 2000-3000mg / L; adjusting the pH value to 5.5-6.5, inoculating hydrogenogens, and fermenting to produce hydrogen; s3, absorbing tail gas generated in the fermentation process with alkali liquor to obtain purified hydrogen, and collecting the purified hydrogen; and S4, loading a filler in an up-flow packed bed reactor, inoculating hydrogen autotrophic denitrifying bacteria, injecting hydrogen into the reactor through a hollow fiber membrane gas distribution device, enabling the bubble diameter to be less than or equal to 50 microns, controlling the water temperature to be 30-35 DEG C, and controlling the HRT to be 4-12 hours. Hydrogen is obtained through fermentation of organic waste and used for a denitrification system, the hydrogen utilization rate is increased, energy consumption is reduced, and the green circulation target of treating waste with waste is achieved.

The invention relates to a method for producing hydrogen alkane through two-stage anaerobic fermentation of lincomycin fungus residues, and belongs to the technical field of biochemistry. The invention aims to improve the bioenergy conversion efficiency of the antibiotic mushroom dregs, reduce the harm caused by residues of produced antibiotics and reduce the cost of innocent treatment of the antibiotic mushroom dregs. The invention provides a method for producing hydrogen and methane in two stages by utilizing lincomycin fungus residues. The method comprises the following steps: carrying out hydrothermal pretreatment on the lincomycin fungus residues, and taking supernate; selecting an activated fermentation liquid as an inoculum, inoculating the inoculum into the supernatant, and carrying out first-stage anaerobic fermentation to obtain hydrogen; biogas slurry of an anaerobic fermentation methane production system or sludge of an anaerobic fermentation link in the antibiotic wastewater sewage treatment process is selected as seed sludge to be inoculated into residues at the hydrogen production fermentation tail end, and second-stage anaerobic fermentation is performed to obtain methane. Development of a novel efficient pretreatment technology and a fermentation mode becomes a critical path for breaking through the bottleneck of the current technology, and a high-quality substrate is provided for subsequent anaerobic fermentation.

The application belongs to the technical field of biological fermentation, and particularly relates to a dark fermentation mixed flora, a culture method thereof and application of the dark fermentation mixed flora in hydrogen production from waste oil reservoirs. In the application, a mixed flora capable of producing hydrogen by using waste oil reservoirs is disclosed, the mixed flora comprising Thermotoga hypogea, Thermotoga elfii, Thermus sp., Propionibacterium freudenreichii, Butyrivibrio fibrisolvens and Pseudomonas aeruginosa. The mixed flora can not only produce hydrogen by using crude oil as raw material, but also improve the flowability of the oil layer, improve the interface between the oil layer and the water layer, and crack heavy components in the crude oil, so that the oil layer can be fully utilized for fermentation hydrogen production, and the efficiency of hydrogen production by microorganisms using waste oil reservoirs is improved.

The present application relates to the field of biological hydrogen production, and particularly provides a lignocellulose biomass circulating and regenerative biochar preparation method and application thereof.More specifically, the present application improves the effect of anaerobic fermentation hydrogen production by using the solid residue containing biochar at the end of fermentation as an additive through circulating and baking.The biochar prepared by the present application is a biochar-based material which significantly improves the performance of biomass anaerobic fermentation on the basis of environmental friendliness.The method provided by the present application has a remarkable effect of resource utilization, is simple to operate, can realize element circulation in the field of fermentation hydrogen production, and provides a new perspective for the resource utilization and energy utilization of agricultural waste biomass.

The invention discloses a method for producing hydrogen and methane by injecting carbon dioxide to reinforce anaerobic fermentation of coal-series mud shale, which comprises the following steps: adopting a stainless steel tank as an anaerobic fermentation reactor, adding a coal-series mud shale sample, injecting fermentation liquor containing indigenous flora and exogenous CO2, carrying out a fermentation experiment under the conditions of reservoir temperature and pressure, and regularly taking a gas sample and a liquid sample for detection. The method comprises the following steps of: testing the generation amount of H2 and CH4, calculating the CO2 stage and accumulated utilization amount, analyzing a liquid phase product and a flora structure, analyzing the enhancement effect of exogenous CO2 injection on H2 generation of a fermentation system and the CO2 biological methanation effect, and evaluating the enhancement effect of exogenous CO2 injection on biological methane output of the fermentation system. By adopting the method for injecting the exogenous CO2, the generation of H2 in the anaerobic fermentation system of the coal-series shale can be promoted, the CO2 conversion and the hydrogen nutrition type methane production can be enhanced, the yield of biological methane can be remarkably increased, and an efficient synergistic way is provided for the yield increase of coal-series gas and the resource utilization of CO2.

The invention discloses a nitrogen limitation-based clostridium pasteurianum-rhodobacter sphaeroides coupling fermentation hydrogen production method and application thereof. The method comprises the following steps: performing dark fermentation on activated clostridium pasteurianum in a nitrogen-free culture medium in an argon environment to produce hydrogen; and adjusting the pH value of the obtained culture solution to 7.0-8.0, selectively sterilizing, inoculating activated rhodobacter sphaeroides, and carrying out light fermentation in an argon environment to produce hydrogen. The clostridium pasteurianum fermentation liquor can be directly subjected to rhodobacter sphaeroides photofermentation hydrogen production culture only by simply adjusting the pH value without operations such as centrifugal removal of thalli. According to the method, excessive proliferation of thalli is inhibited through nitrogen limitation, the flow direction of a carbon source is saved, high expression of nitrogenase is induced, and a second efficient hydrogen production way except hydrogenase is opened up, so that the hydrogen yield is further increased while the cost of a nitrogen source is reduced. The hydrogen production yield reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The application discloses a method for producing hydrogen through Clostridium pasteurianum-Rhodobacter sphaeroides coupling fermentation based on nitrogen limitation and application thereof. In the method, activated Clostridium pasteurianum is subjected to dark fermentation for producing hydrogen in a nitrogen-free culture medium and in an argon environment; then, the pH value of the obtained culture solution is adjusted to 7.0-8.0, the activated Rhodobacter sphaeroides is inoculated after selective sterilization, and the light fermentation for producing hydrogen is carried out in the argon environment. In the application, the fermentation solution of Clostridium pasteurianum does not need to be subjected to centrifugal removal of bacterial bodies and the like, and only needs to be simply adjusted in pH value, so that the light fermentation for producing hydrogen of Rhodobacter sphaeroides can be directly carried out. In the application, the nitrogen limitation is used to inhibit excessive proliferation of bacterial bodies, save carbon source flow, induce high expression of nitrogen fixation enzyme, and open a second high-efficiency hydrogen production path in addition to hydrogenase, so that the hydrogen production yield is further improved while the cost of nitrogen source is reduced. The hydrogen production yield of the application reaches 9.81 mol H2 / mol glucose, which is the highest level at present.

The invention relates to the field of organic solid waste biological treatment, in particular to a method for enhancing hydrogen production from kitchen waste. The invention provides a method for enhancing hydrogen production from kitchen waste, which comprises the following steps: mixing kitchen waste with a conduction proton material, and carrying out anaerobic fermentation. On one hand, an exogenous conduction proton material is adopted to enhance directional transfer of extracellular protons in the hydrogen production process through fermentation of the kitchen waste, the utilization efficiency of the extracellular protons is improved, the hydrogenase activity is improved, and the hydrogen production amount through anaerobic fermentation of the kitchen waste is remarkably increased; on the other hand, anaerobic fermentation is adopted, and the effects of creating a dominant metabolism environment for hydrogenogens, directionally and rapidly converting organic matters to generate hydrogen and avoiding methane bacterium enrichment from the source can be achieved. On the basis, the method provided by the invention has important significance in improving the energy utilization of the kitchen waste.

The invention discloses a salt-tolerant amylase producing strain and application of a complex microbial inoculant thereof in efficient anaerobic fermentation hydrogen production of kitchen wastewater. According to the invention, a salt-tolerant amylase producing strain, namely bacillus paralicheniformis Q4 (CCTCC NO: M 20241043), is obtained through separation and screening, and the bacillus paralicheniformis Q4 can be used for producing the salt-tolerant amylase. According to the present invention, with the complex microbial inoculum prepared by compatibility of the complex microbial inoculum with activation solutions of three strains such as a salt-tolerant fermentation hydrogen production bacterium-bacillus altitudinis K3 (CCTCC NO: M 20241042) and a salt-tolerant protease production bacterium-arthrobacter protophila A1 (CCTCC NO: M 20241041) according to a volume ratio of (1-2): (1-2): 1, the fermentation hydrogen production efficiency of the kitchen wastewater can be synergistically enhanced, and the hydrogen yield and the production intensity can respectively achieve more than 100 mL / g-COD and more than 720 L / m < 3 > / d;

The invention discloses a method for enhancing hydrogen production of anaerobacter thermosaccharolyticus by using polyaluminum chloride, and belongs to the technical field of hydrogen production by microbial fermentation. A method for enhancing hydrogen production of anaerobacter thermosaccharolyticus by using polyaluminum chloride comprises the following steps: S1, preparation of a seed solution: inoculating a strain into a seed culture medium for activation and amplification culture to obtain the seed solution; and S2, fermentation hydrogen production: inoculating the seed solution into a fermentation culture medium containing polyaluminum chloride, and carrying out anaerobic fermentation to obtain hydrogen. According to the method, the high-performance hydrogen production strain is combined with a polyaluminum chloride strengthening strategy, so that a considerable hydrogen production effect is obtained, and important technical support and reference value are provided for resource utilization of biomass wastes and industrial production of clean hydrogen energy.

This invention provides a method for hydrogen production from organic wastewater through dark fermentation, belonging to the field of fermentation hydrogen production technology. The method includes: mixing organic wastewater, biochar loaded with metal nanoparticles, and a domesticated butyric acid-producing mixed bacterial community; performing micro-electrolysis and bioactivation treatment to obtain a pretreated solution; then subjecting the pretreated solution to hydrogen-producing phase fermentation treatment, adding an electron mediator to obtain a fermentation broth containing volatile acids; further, subjecting the fermentation broth to de-inhibition phase treatment, using adsorbent materials to adsorb volatile acids, and simultaneously converting some of the adsorbed volatile acids into butyric acid through acid-producing bacteria to obtain a post-fermentation solution; electrolyzing the post-fermentation solution, utilizing electroactive hydrogen-producing bacteria for extracellular electron transfer to convert residual organic matter in the solution into hydrogen gas to obtain crude hydrogen, which is then purified to obtain hydrogen gas. This invention solves the problems of low utilization rate of organic wastewater, inhibition of hydrogen production by volatile acids, and the impact of environmental parameter fluctuations on yield in existing processes.

The invention discloses a method for producing hydrogen through sludge bioelectricity fermentation and recycling blue iron ore based on signal molecule-electron shuttle cooperative regulation and control, and belongs to the technical field of waste biomass energy and resource recycling. The method comprises the following steps: (1) in an inoculation starting and functional microorganism domestication stage, adding quorum sensing signal molecules C8-HSL to promote directional enrichment of electroactive bacteria on an electrode interface and induce ordered growth of an anode biological membrane; and (2) in the stable operation stage of the system, VFAs obtained through hot alkali pretreatment is used as a reaction substrate, electron shuttle humic acid is added, transmembrane / extracellular electron transfer is enhanced, Fe < 2 + > is mildly released from an iron anode and directionally crystallized with phosphate radicals to generate high-purity blue iron ore, and the cathode synchronously realizes efficient hydrogen evolution. According to the invention, through staged addition and dosage control, a'guiding-enhancing 'two-stage cooperation path is constructed, the integrated target of quick start, hydrogen production enhancement and phosphorus selective recovery is realized, and a new idea is provided for residual sludge recycling.

The invention relates to the technical field of synthesis and anaerobic fermentation of nano composite materials, in particular to a method for enhancing anaerobic fermentation hydrogen production through a calcium oxide loaded ferroferric oxide nano material. Ferroferric oxide nano-particles are loaded on calcium oxide, the loading proportions of ferroferric oxide are respectively 10%, 20%, 30% and 40%, and the calcium oxide loaded ferroferric oxide nano composite material is introduced into a hydrogen production working solution enriched with anaerobic fermentation hydrogen production microorganisms. The material can synchronously realize two core effects: ferroferric oxide strengthens electron transfer to assist microbial enrichment, calcium oxide strengthens hydrolysis of organic matters and regulates and controls pH to relieve excessive acidification of a system, and finally, effective support is provided for improvement of anaerobic fermentation hydrogen production efficiency.

The invention belongs to the technical field of sewage treatment, and provides a microplastic depolymerization enhanced bioelectricity fermentation hydrogen production method, which comprises the following steps: mixing a sludge sample and a sodium hydroxide solution, adjusting the pH value, and sequentially carrying out pretreatment and anaerobic fermentation to obtain a fermentation liquid; carrying out aging treatment on the micro-plastic to obtain an aging solution; sludge and a buffer solution are mixed and sequentially subjected to primary operation and secondary operation, and a microbial electric fermentation system is obtained; and inoculating the aqueous solution of the aging solution and the fermentation solution into a microbial electric fermentation system, adjusting the pH value, and carrying out fermentation to produce hydrogen. According to the method, the sludge is pretreated by adopting the hot alkali, sludge flocs and cell walls of microorganisms are destroyed, the dissolution rate of the sludge is increased, the anaerobic fermentation process is accelerated, generation of short-chain fatty acid is promoted, and resource and energy recovery maximization is achieved. Meanwhile, microplastic depolymerization and bioelectrochemical hydrogen production are coupled for the first time, the cathode is synchronously driven to efficiently produce hydrogen, the substrate limitation and the energy conversion bottleneck of traditional biological hydrogen production are broken through, and carbon emission can be reduced.

The present application relates to a kind of high-efficiency denitrification method based on anaerobic hydrogen production coupling hydrogen-type autotrophic denitrification, it includes S1: using the high concentration organic wastewater of COD concentration ≥5000mg / L, by mechanical crushing and homogenization processing ensure that particle size ≤1mm, ultrasonic treatment is strengthened organic matter dissolution;S2: first to wastewater inoculation acid-producing bacteria, at 34-38 ℃ continuous stirring fermentation is carried out, so that acid-producing bacteria is converted into volatile fatty acid VFA into complex organic matter, VFA concentration reaches 2000-3000mg / L;Again, adjust pH to 5.5-6.5, inoculation hydrogen-producing bacteria and carry out fermentation hydrogen production;S3: tail gas generated in fermentation process is absorbed after alkali liquor, obtain purified hydrogen, collection;S4: in upflow packed bed reactor, load packing and inoculate hydrogen autotrophic denitrifying bacteria, hydrogen is injected into reactor by hollow fiber membrane gas distribution device, so that bubble diameter ≤50 μm, control water temperature 30-35 ℃, HRT is 4-12h.The present application obtains hydrogen by organic waste fermentation for denitrification system, and improves hydrogen utilization, saves energy consumption, realizes the green recycling goal of waste treatment.

The invention discloses a method for producing hydrogen by converting tobacco waste fermentation hydrogen production wastewater through a microbial electrolysis cell, which comprises the following steps: (1) pretreating tobacco waste, naturally airing and crushing broken tobacco leaves and the like, and sieving; (2) preparing a clostridium perfringens seed culture medium and culturing a seed solution; (3) inoculating the seed solution into a dark fermentation culture medium according to an inoculum size of 10-20%, and fermenting at 35 + / -1 DEG C and 120 r / min to obtain wastewater containing acetic acid and the like, the dark fermentation culture medium containing the pretreated tobacco waste and the like and having a pH value of 6.47; (4) preparing a microbial electrolysis cell inoculation bacterium source, wherein the microbial electrolysis cell inoculation bacterium source is prepared by performing dilution, microwave pretreatment and the like on cow dung or sheep manure compost; (5) enriching the anode by taking the diluted fermentation wastewater as a substrate in the single-chamber air cathode microbial fuel cell; and (6) transferring the anode to a microbial electrolysis cell reactor, blowing by using the diluted wastewater as a substrate, and producing hydrogen at 36 + / -1 DEG C and 0.4-0.9 V. The method can utilize the tobacco waste and the fermentation wastewater to relieve pollution, realize stepped hydrogen production and improve the hydrogen yield.

The application provides a composite microbial inoculum for hydrogen production by fermentation and application thereof. The composite microbial inoculum comprises Priestia aryabhattai YLS2, Niallia nealsonii YLS4 and Lysinibacillus boronitolerans S5; wherein the preservation number of the Priestia aryabhattai YLS2 is CGMCC NO.32138, the preservation number of the Niallia nealsonii YLS4 is CGMCC NO.32137, and the preservation number of the Lysinibacillus boronitolerans S5 is CGMCC NO.32139. The hydrogen production process is optimized by the synergistic effect of multiple microorganisms, the composite microbial inoculum for hydrogen production by fermentation can improve the hydrogen production and the stability in the gas production process, and has significant environmental benefits and economic potential.