4 results about "Fermentative hydrogen production" patented technology

The application discloses a method for improving hydrogen production performance of Clostridium butyricum by using hematite nanoparticles, and belongs to the technical field of biological hydrogen production and microbial metabolism regulation. The method improves the hydrogen production performance by adding hematite nanoparticles into an anaerobic fermentation hydrogen production system of Clostridium butyricum for anaerobic fermentation. The application first discloses a specific double regulation mechanism of hematite nanoparticles on hydrogen production of Clostridium butyricum. By using hematite nanoparticles as a core regulation material, and through precise concentration gradient addition and stage temperature synergistic regulation, the hydrogen production lag period can be greatly shortened, the initial hydrogen production rate can be improved, byproduct synthesis of butyric acid can be inhibited, and the efficient hydrogen production path of acetic acid can be strengthened. The core technical problems that existing iron-based additives cannot shorten the hydrogen production lag period, the initial hydrogen production efficiency is low, and product feedback inhibition is easily caused are solved. The method is simple to operate, has strong industrialization adaptability, and can be widely applied to industrialization batch and continuous production of dark fermentation biological hydrogen production.

This invention provides a method for hydrogen production from organic wastewater through dark fermentation, belonging to the field of fermentation hydrogen production technology. The method includes: mixing organic wastewater, biochar loaded with metal nanoparticles, and a domesticated butyric acid-producing mixed bacterial community; performing micro-electrolysis and bioactivation treatment to obtain a pretreated solution; then subjecting the pretreated solution to hydrogen-producing phase fermentation treatment, adding an electron mediator to obtain a fermentation broth containing volatile acids; further, subjecting the fermentation broth to de-inhibition phase treatment, using adsorbent materials to adsorb volatile acids, and simultaneously converting some of the adsorbed volatile acids into butyric acid through acid-producing bacteria to obtain a post-fermentation solution; electrolyzing the post-fermentation solution, utilizing electroactive hydrogen-producing bacteria for extracellular electron transfer to convert residual organic matter in the solution into hydrogen gas to obtain crude hydrogen, which is then purified to obtain hydrogen gas. This invention solves the problems of low utilization rate of organic wastewater, inhibition of hydrogen production by volatile acids, and the impact of environmental parameter fluctuations on yield in existing processes.

The present application relates to a kind of high-efficiency denitrification method based on anaerobic hydrogen production coupling hydrogen-type autotrophic denitrification, it includes S1: using the high concentration organic wastewater of COD concentration ≥5000mg / L, by mechanical crushing and homogenization processing ensure that particle size ≤1mm, ultrasonic treatment is strengthened organic matter dissolution;S2: first to wastewater inoculation acid-producing bacteria, at 34-38 ℃ continuous stirring fermentation is carried out, so that acid-producing bacteria is converted into volatile fatty acid VFA into complex organic matter, VFA concentration reaches 2000-3000mg / L;Again, adjust pH to 5.5-6.5, inoculation hydrogen-producing bacteria and carry out fermentation hydrogen production;S3: tail gas generated in fermentation process is absorbed after alkali liquor, obtain purified hydrogen, collection;S4: in upflow packed bed reactor, load packing and inoculate hydrogen autotrophic denitrifying bacteria, hydrogen is injected into reactor by hollow fiber membrane gas distribution device, so that bubble diameter ≤50 μm, control water temperature 30-35 ℃, HRT is 4-12h.The present application obtains hydrogen by organic waste fermentation for denitrification system, and improves hydrogen utilization, saves energy consumption, realizes the green recycling goal of waste treatment.