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Method for culturing marine photosynthetic bacteria used for light-dark fermentation and coupling hydrogen production

A technology of photosynthetic bacteria and photofermentation, which is applied in the marine field to achieve the effects of reducing costs, improving utilization, and reducing environmental pollution

Inactive Publication Date: 2008-02-27
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the method of coupling hydrogen production with light-dark fermentation for the cultivation of marine photosynthetic bacteria from sludge and sewage in marine farms

Method used

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  • Method for culturing marine photosynthetic bacteria used for light-dark fermentation and coupling hydrogen production
  • Method for culturing marine photosynthetic bacteria used for light-dark fermentation and coupling hydrogen production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Hydrogen production by photoculture (see Figure 1):

[0039] ① Enrichment culture: Take samples from the sludge of marine farms to cultivate marine photosynthetic bacteria. Immediately after sampling, add 50ml of the sample to 500ml of the screening medium designed by the applicant for enrichment culture, and fill the entire culture bottle as much as possible to Reduce the amount of air in the culture flask. Nitrogen gas was always blown to the sample during the whole operation, and nitrogen gas was blown to the culture bottle for 15 minutes before closing the rubber stopper of the culture bottle to achieve anaerobic culture conditions for 3 days. The culture conditions are as follows: the temperature of the incubator is 28° C., and the light intensity is 2500 lux. Wherein: the culture medium is a liquid culture medium and its components are: ammonium chloride 1.0g, sodium acetate 2.0g, yeast powder 0.2g, peptone 0.1, sodium bicarbonate 1, seawater 1000ml, pH 7.5; t...

Embodiment 2

[0053] The difference from Example 1 is:

[0054] 1) Photoculture hydrogen production:

[0055] ①Enrichment culture: Take samples from the sludge of marine farms to cultivate marine photosynthetic bacteria. Immediately after sampling, add 40ml of the sample to 400ml of the screening medium designed by the applicant for enrichment culture, and cultivate in anaerobic conditions for 4 days. The conditions are: the temperature of the incubator is 35° C., and the light intensity is 2500 lux. Wherein: the culture medium is liquid culture medium composition is: ammonium chloride 2.0g, sodium acetate 4.0g, yeast powder 1.0g, peptone 0.5g, sodium bicarbonate 2g, seawater 1000ml, pH8.2; Then repeat above-mentioned enrichment culture 3 The next time, the enriched medium turns red or purple, and Rhodoseudomonas capsulata and Rhodobacter sphaeroides appear.

[0056] ②Hydrogen production culture: Streak the inoculum after the enrichment culture to pick a single colony. The medium at this ...

Embodiment 3

[0064] The difference from Example 1 is:

[0065] 1) Photoculture hydrogen production:

[0066] ①Enrichment culture: Immediately after sampling, 15ml of the sample was added to 60ml of the screening medium designed by the applicant, and the enrichment culture was cultured under anaerobic conditions for 4 days. The culture conditions were: the temperature of the incubator was 30°C, and the light intensity was 3500 lux. Wherein: medium is liquid medium composition and is: ammonium chloride 4.5g, sodium acetate 2.5g, yeast powder 0.5g, peptone 0.5g, sodium bicarbonate 1.5g, seawater 1000ml, pH 7.8; Then repeat above-mentioned enrichment culture 3 Second, the enriched medium turns red or purplish red, Rhodoseudomonas capsulata, Rhodobacter sphaeroides.

[0067] ②Hydrogen production culture: Streak the inoculum after the enrichment culture to pick a single colony. The medium at this time is 1.8 grams of agar added to the hydrogen production medium. The culture conditions: add 1.5 ...

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Abstract

The invention relates to a method for light-blind-fermentation-coupling-producing hydrogen by culturing sea photosynthetic bacterium with sludge and effluent of sea cultivation section, The method comprises the following steps: proceeding with culturing without light in order to produce hydrogen with sludge and effluent of sea cultivation as the culture medium; culturing with light; proceeding with coupling-production hydrogen by adding light fermenting bacteria in the culture medium in order to improve the releasing quantity of hydrogen and recover the production-hydrogen activity of the sea blind fermentation production-hydrogen bacterial. The method not only reduces the pollution of the environment, but also can release the present nervous energy crisis pressure.

Description

technical field [0001] The invention relates to the field of marine technology, in particular to a method for cultivating marine photosynthetic bacteria with light-dark fermentation coupled hydrogen production using sludge and sewage from seawater farms. Background technique [0002] At present, "fossil energy" is gradually exhausted, and the products formed by its combustion (such as CO 2 and SO 2 ) etc. will cause serious environmental pollution (such as greenhouse effect and acid rain). Therefore, we must look for clean renewable energy to replace "fossil energy". Due to its high energy density, convenient transportation, and no pollution, hydrogen energy is most likely to become one of the main alternative energy sources in the future. Among various hydrogen production methods, biological fermentation hydrogen production has become one of the most promising hydrogen production methods due to its advantages of low energy consumption, high efficiency, and no pollution. ...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12R1/145C12R1/26C12R1/01
Inventor 王广策才金玲周百成
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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