Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation

A simultaneous saccharification and fermentation, lignocellulose technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of fermentation inhibitors, pollute the environment, and high energy demand, and achieve cost reduction and equipment reduction Investment, the effect of reducing energy consumption

Active Publication Date: 2012-01-18
HARBIN INST OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problems of high energy demand for lignocellulose pretreatment, environmental pollution and production of fermentation inhibitors in the existing fermentation hydrogen production process, and provides a method for biological pretreatment of lignocellulose and synchronous saccharification and fermentation hydrogen production

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  • Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation
  • Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation
  • Method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation

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specific Embodiment approach 1

[0014] Specific Embodiment 1: In this embodiment, the method for biological pretreatment of lignocellulose and synchronous saccharification and fermentation for hydrogen production is realized according to the following steps:

[0015] 1. Inoculate white rot fungi in lignocellulose liquid medium, culture in suspension under aerobic conditions at 29°C for 10 to 20 days, then wash with water, and dry in an oven at 105°C to constant weight to obtain pretreatment cellulose; 2. Mix 3L hydrogen-producing bacteria nutrient salt solution with 7L Trichoderma viride crude enzyme solution, add 150-350g pretreated cellulose to obtain synchronous saccharification and fermentation hydrogen production medium, and then pass it into % nitrogen, then inoculate the seed solution of hydrogen-producing bacteria, and carry out anaerobic fermentation to produce hydrogen for 24-72 hours under the conditions of initial pH value of 6.5, temperature of 60°C, and stirring speed of 150r / min; that is, the b...

specific Embodiment approach 2

[0026] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: in step 1, the inoculation of white rot fungi adopts a concentration of 2×10 6 spores / ml of spore suspension, the inoculation volume of each bottle is 2×10 7 spores. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0027] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the hydrogen-producing bacteria in the seed liquid of hydrogen-producing bacteria in step 2 is thermosaccharolyticum anaerobic bacillus W16 (Thermoanaerobacterium thermosaccharolyticum W16), and Thermoanaerobacterium thermosaccharolyticum W16 was established in 2008 It is disclosed in an article titled "Dark fermentation of xylose and glucose mix using isolated Thermoanaerobacterium thermosaccharolyticum W16" published in "International Journal of Hydrogen Energy", No. 33, pages 6124-6132. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a method for biologic pretreatment of lignocellulose and hydrogen production through simultaneous saccharification and fermentation, relates to a method for pre-treating lignocellulose and producing hydrogen through fermentation, and solves the problems of high energy requirement, environmental pollution and production of a fermentation inhibitor existing in the pretreatment of the lignocellulose during the conventional fermentative hydrogen production. The method comprises the following steps of: 1, inoculating white rot fungi in lignocellulose liquid culture medium to culture, washing and drying to obtain pretreated cellulose; and 2, mixing hydrogenogens nutritive salt solution and environmentally-friendly trichoderma rough enzyme solution, and adding into the pretreated cellulose to obtain simultaneous saccharification and fermentation hydrogen production culture medium; then introducing nitrogen; and inoculating seed liquid of hydrogenogens to perform anaerobic fermentation to produce hydrogen. By adopting the method, the energy consumption during lignocellulose hydrogen production is reduced, equipment investment is reduced, the fermentation inhibitor is not produced, damage probably caused to environment during hydrogen production is reduced to the minimum; the relative removal rate of lignin reaches 55.7 percent; and the hydrogen production capacity is 72.6ml / g.

Description

technical field [0001] The invention relates to a method for pretreating lignocellulose and producing hydrogen by fermentation. Background technique [0002] Currently, the world's energy needs are largely met by fossil fuels. However, overwhelming scientific evidence points to the potentially catastrophic effects of this global climate change caused by the indiscriminate use of fossil fuels. Therefore, it is imperative to develop a new type of energy. As a new type of energy carrier, hydrogen has the advantages of recyclability, cleanness and pollution-free, and high combustion value. There are many ways to produce hydrogen, among which the fermentation biological hydrogen production technology has attracted widespread attention due to its high hydrogen production rate and energy saving and environmental protection in the fermentation process. To make fermentative biohydrogen production more economically competitive, seeking a renewable and inexpensive substrate is key. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12P19/14C12R1/01
Inventor 任南琪赵磊曹广丽王爱杰任宏宇
Owner HARBIN INST OF TECH
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