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Method for producing hydrogen by biomass cascade fermentation

A biomass and fermentation technology, applied in fermentation and other directions, can solve the problems of low conversion rate of biological hydrogen production, and achieve the effect of improving the conversion rate of hydrogen and solving the problem of low conversion rate of hydrogen.

Inactive Publication Date: 2009-03-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for hydrogen production by cascade fermentation of biomass in order to solve the problem of low conversion rate of biological hydrogen production in the existing fermentation method

Method used

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Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0007] Specific Embodiment 1: The method for hydrogen production by cascade fermentation of biomass in this embodiment is realized according to the following steps: 1. Anaerobic granular sludge is shaken for 5 to 6 hours to obtain anaerobic activated sludge, which is then placed in a container and filled with nitrogen Seal after 10-20 minutes, heat treatment at 110-130°C for 20-40 minutes; 2. Adjust the pH value of the effluent water from the fermentation process to 7.0-8.0, then add 2-bromoethanesulfonate sodium, and then Pump into the continuous flow stirred tank type acidogenic fermentation hydrogen production reaction equipment as the base material, and then inoculate the anaerobic activated sludge after heat treatment; 3. Continuously fill the continuous flow stirred tank type acidogenic fermentation hydrogen production reaction equipment Nitrogen with a purity of 99.99% and a flow rate of 100ml / min, run for 50 to 60 days under the conditions of a reaction equipment temper...

specific Embodiment approach 2

[0008] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in the step one, the anaerobic granular sludge is shaken and cultivated for 5.5 hours to obtain anaerobic activated sludge, which is then placed in a container, filled with nitrogen for 15 minutes and then sealed. Heat treatment at 120°C for 30min. Other steps and parameters are the same as those in Embodiment 1.

[0009] In this embodiment, the anaerobic granular sludge shaking culture is to put the anaerobic granular sludge into a serum bottle containing dozens of glass beads, seal it with a stopper, and place it in a full-temperature shaking flask at 35°C and 120-180r / min. shake culture in the cabinet.

specific Embodiment approach 3

[0010] Embodiment 3: The difference between this embodiment and Embodiment 1 is that in step 2, the pH value of the effluent of the fermentation biological hydrogen production is adjusted to 7.5. Other steps and parameters are the same as those in Embodiment 1.

[0011] In this embodiment, the reagent used for pH adjustment is KOH or NaOH.

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Abstract

The invention discloses a method for producing hydrogen by the step fermentation of biomass, which relates to a method for producing hydrogen and solves the problem that the transformation rate of the current fermentation method for biological hydrogen production is relatively low. The method comprises the steps: firstly, the shake culture of aerobic granular sludge is carried out, thus obtaining aerobic activated sludge; and then heat treatment is carried out; secondly, water of biological hydrogen production by the fermentation method and disodium bromoethane sulfonate are taken as the bottom materials of reaction equipment, and then the aerobic activated sludge after heat treatment is mutated; and thirdly, nitrogen gas is continuously pumped into the reaction equipment, thus obtaining hydrogen after running. The hydrogen transformation rate of a unit base material in the invention is improved to be more than 2.5molH 2 / mol of hexose.

Description

technical field [0001] The invention relates to a method for producing hydrogen. Background technique [0002] The basic principle of fermentation biological hydrogen production at home and abroad is to use the acid-producing fermentation bacteria in the anaerobic digestion system to ferment and metabolize carbohydrates to produce hydrogen, and degrade organic matter into acetic acid, propionic acid, butyric acid, lactic acid and ethanol, etc. while releasing hydrogen gas. Due to the relatively low degree of conversion or degradation of the substrate by acidogenic fermentation bacteria, only a small part of the hydrogen in the substrate is released in the form of hydrogen gas, while most of the hydrogen still exists in such as propionic acid, butyric acid and ethanol In the end product of the acidogenic fermentation liquid phase, the hydrogen conversion rate of biomass is greatly limited, so that the hydrogen conversion rate per unit substrate in the hydrogen production sys...

Claims

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Application Information

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IPC IPC(8): C12P3/00
Inventor 李建政昌盛王淑静李伟光
Owner HARBIN INST OF TECH
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