Method for improving hydrogen yield of hydrogenogens

A production and strain technology, applied in the field of microbial technology application, can solve problems such as no relevant reports, and achieve the effects of reducing costs, increasing hydrogen production, and promoting growth

Active Publication Date: 2018-04-13
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no relevant report on how to increase the hydrogen production of acetic-type fermentative hydrogen-producing Clostridium (C.pasteurianum) at this stage.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving hydrogen yield of hydrogenogens
  • Method for improving hydrogen yield of hydrogenogens
  • Method for improving hydrogen yield of hydrogenogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Strain acquisition and cultivation:

[0028] C. pasteurianum (Clostridium pasturii) was purchased from the American Tissue Culture Collection (ATCC), and the deposit number is: 7041. Culture conditions, medium components: tryptone: 0.5g / L, peptone: 0.5g / L, NaCl: 5g / L, KH 2 PO 4 : 0.544g / L, K 2 HPO 4 : 2.10g / L, trace mineral salt: 10mL / L (see Table 1 for details), vitamin solution: 10mL / L (see Table 2 for details). PH=7.0, 37°C, culture in the dark.

[0029] Table 1

[0030]

[0031]

[0032] Table 2

[0033] Vitamin composition (100×)

mg / L

Biotin

2

folic acid

2

pyridoxine HCl

10

Thiamine HCl

5

riboflavin

5

nicotinic acid

5

calcium D-(+)-pantothenate

5

cyanocobalamine

0.1

p-aminobenzoic acid

5

thioctic acid

5

[0034] 2) Laboratory synthesis of magnetite and ferrihydrite:

[0035] Synthesize magnetite with reference to the method of Young e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of microbial technology application, and particularly relates to a method for improving the hydrogen yield of hydrogenogens. The hydrogenogens strains are inoculatedinto a culture medium containing 2.5 to 20 mmol / L ferriferous oxides; through dark fermentation culture, the great-quantity hydrogen generation of the hydrogenogens strains can be realized. Through the addition of ferrihydrite and magnetite, the growth of hydrogen production clostridium C.pasteurianum is promoted; the C.pasteurianum substrate conversion efficiency is accelerated; the yield of metabolite products of hydrogen gas, acetic acid and butyric acid is improved. The method provided by the invention has the advantages that the hydrogen yield of the hydrogen production clostridium is greatly improved; the theoretical support is provided for the large-scale production of microbial dark fermentation hydrogen production.

Description

technical field [0001] The invention belongs to the field of microbial technology application, and specifically relates to a method for increasing the hydrogen production of hydrogen-producing bacteria. Background technique [0002] Hydrogen production by microbial dark fermentation is a biohydrogen production method with application potential. However, the slow rate and low yield are still the main reasons restricting the large-scale production and application of this hydrogen production method. Therefore, it is necessary to find new methods with high substrate conversion Hydrogen-producing strains and methods to improve the high-efficiency hydrogen production of hydrogen-producing strains are urgent problems to be solved in the field of microbial dark fermentation hydrogen production. [0003] Hydrogen is a clean energy with a high heat of combustion (122kJ / g). However, the main way of industrial hydrogen production is still through high-temperature reforming of natural ga...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/145
CPCC12P3/00
Inventor 刘芳华张月超肖雷雷王欧美
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap