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Method for improving hydrogen yield of hydrogenogens

A production and strain technology, applied in the field of microbial technology application, can solve problems such as no relevant reports, and achieve the effects of reducing costs, increasing hydrogen production, and promoting growth

Active Publication Date: 2018-04-13
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no relevant report on how to increase the hydrogen production of acetic-type fermentative hydrogen-producing Clostridium (C.pasteurianum) at this stage.

Method used

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  • Method for improving hydrogen yield of hydrogenogens
  • Method for improving hydrogen yield of hydrogenogens
  • Method for improving hydrogen yield of hydrogenogens

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Experimental program
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Effect test

Embodiment 1

[0027] 1) Strain acquisition and cultivation:

[0028] C. pasteurianum (Clostridium pasturii) was purchased from the American Tissue Culture Collection (ATCC), and the deposit number is: 7041. Culture conditions, medium components: tryptone: 0.5g / L, peptone: 0.5g / L, NaCl: 5g / L, KH 2 PO 4 : 0.544g / L, K 2 HPO 4 : 2.10g / L, trace mineral salt: 10mL / L (see Table 1 for details), vitamin solution: 10mL / L (see Table 2 for details). PH=7.0, 37°C, culture in the dark.

[0029] Table 1

[0030]

[0031]

[0032] Table 2

[0033] Vitamin composition (100×)

mg / L

Biotin

2

folic acid

2

pyridoxine HCl

10

Thiamine HCl

5

riboflavin

5

nicotinic acid

5

calcium D-(+)-pantothenate

5

cyanocobalamine

0.1

p-aminobenzoic acid

5

thioctic acid

5

[0034] 2) Laboratory synthesis of magnetite and ferrihydrite:

[0035] Synthesize magnetite with reference to the method of Young e...

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Abstract

The invention belongs to the field of microbial technology application, and particularly relates to a method for improving the hydrogen yield of hydrogenogens. The hydrogenogens strains are inoculatedinto a culture medium containing 2.5 to 20 mmol / L ferriferous oxides; through dark fermentation culture, the great-quantity hydrogen generation of the hydrogenogens strains can be realized. Through the addition of ferrihydrite and magnetite, the growth of hydrogen production clostridium C.pasteurianum is promoted; the C.pasteurianum substrate conversion efficiency is accelerated; the yield of metabolite products of hydrogen gas, acetic acid and butyric acid is improved. The method provided by the invention has the advantages that the hydrogen yield of the hydrogen production clostridium is greatly improved; the theoretical support is provided for the large-scale production of microbial dark fermentation hydrogen production.

Description

technical field [0001] The invention belongs to the field of microbial technology application, and specifically relates to a method for increasing the hydrogen production of hydrogen-producing bacteria. Background technique [0002] Hydrogen production by microbial dark fermentation is a biohydrogen production method with application potential. However, the slow rate and low yield are still the main reasons restricting the large-scale production and application of this hydrogen production method. Therefore, it is necessary to find new methods with high substrate conversion Hydrogen-producing strains and methods to improve the high-efficiency hydrogen production of hydrogen-producing strains are urgent problems to be solved in the field of microbial dark fermentation hydrogen production. [0003] Hydrogen is a clean energy with a high heat of combustion (122kJ / g). However, the main way of industrial hydrogen production is still through high-temperature reforming of natural ga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/145
CPCC12P3/00
Inventor 刘芳华张月超肖雷雷王欧美
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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