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Method for producing hydrogen by syntrophism and interaction of fermentative hydrogen-producing bacteria and electroactive bacteria

An electroactive and bacterial technology is applied in the field of hydrogen production where fermentative hydrogen-producing bacteria and electroactive bacteria interact with each other. , Improve the efficiency of hydrogen production and reduce the effect of metabolic inhibition

Active Publication Date: 2019-12-24
HARBIN INST OF TECH
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  • Claims
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Problems solved by technology

[0004] In order to solve the problem that the existing anaerobic biological hydrogen production process is inhibited by bacterial metabolism and substrate utilization is not complete, which further affects the hydrogen production efficiency, the invention provides a hydrogen production method in which fermentative hydrogen production bacteria and electroactive bacteria interact with each other method

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  • Method for producing hydrogen by syntrophism and interaction of fermentative hydrogen-producing bacteria and electroactive bacteria

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specific Embodiment approach 1

[0019] Specific embodiment 1: This embodiment provides a method for utilizing Harbin ethanologens (Ethanoligenens harbinense) W1 and Geobacter (Geobacter sulfurreducens) PCA intertrophy interaction and interspecific electron transfer to efficiently produce hydrogen. The operation steps are as follows:

[0020] (1) Cultivate Harbin Ethanologenus W1 by anaerobic culture method: high-purity N with a purity of 99.99% after high temperature boiling 2 The liquid medium A after aeration and high-temperature and high-pressure steam sterilization was used to cultivate Harbin ethanologenic bacteria W1, and the anaerobic bottle inoculated with Harbin ethanologenic bacteria W1 was placed in a constant temperature shaker at 35°C and 170rpm for shaking culture. After 46 hours, In the anaerobic bottle, a light yellow turbid bacterial liquid accompanied by white flocculent bacterial cells was formed, centrifuged at a speed of 5000r / h for 10 minutes, discarded the supernatant, and obtained the...

specific Embodiment approach 2

[0031] Embodiment 2: In this embodiment, a method for producing hydrogen by Ethanoligenensharbinense W1 is provided. The main difference between Embodiment 2 and Embodiment 1 is that only Ethanologens Harbin W1 is inoculated in step (3). The other culture conditions and steps are the same. After 1.5 hours of culture, gas production begins, and the maximum hydrogen production rate is 32.8mL / L-medium h in about 35 hours. The final hydrogen production rate is 1.08mol-hydrogen / mol-glucose.

[0032] The hydrogen production effect of specific embodiment 1 and specific embodiment 2 is compared in the following table,

[0033]

[0034] It can be seen from the above table that the co-cultivation of Harbin Ethanologenus W1 and Geobacter PCA can significantly shorten the gas production time, and the maximum hydrogen production rate is significantly increased, and has no adverse effect on the substrate utilization rate. The co-cultivation can obtain higher Hydrogen yield.

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Abstract

The invention relates to a method for producing hydrogen by syntrophism and interaction of fermentative hydrogen-producing bacteria and electroactive bacteria, in particular to a method for realizinghigh-efficiency hydrogen production by utilizing syntrophism and interaction of fermentative hydrogen-producing bacteria and electroactive bacteria and inter-specific electron transfer, and belongs tothe technical field of hydrogen production by fermentation. The method aims to solve problems that the existing anaerobic biological hydrogen production process is inhibited by bacterial metabolism,substrate is not completely utilized, and then hydrogen production efficiency is influenced. According to the method, Ethanoligenens harbinense W1 and Geobacteria PCA are co-cultured, terminal metabolites such as acetic acid and ethanol produced by the hydrogen-producing bacteria in the fermentation system are consumed by the electroactive bacteria, so that metabolic inhibition among the bacteriain a closed fermentation system is reduced, and continuous and efficient hydrogen production is promoted. In addition, glucose can be used as a carbon source in the method, and the glucose only can beutilized only by the fermentative hydrogen-producing bacteria, so that substrate competition is reduced, and the produced hydrogen has higher yield and higher purity.

Description

technical field [0001] The present invention relates to a method for producing hydrogen through mutual support and interaction between fermentative hydrogen-producing bacteria and electroactive bacteria, and in particular to a method for realizing efficient hydrogen production by using mutual support and interaction between fermentative hydrogen-producing bacteria and electroactive bacteria and interspecies electron transfer The method belongs to the technical field of fermentation hydrogen production. Background technique [0002] Hydrogen energy is a clean and environmentally friendly energy with high energy density and a wide range of sources, which is superior to traditional fossil fuels. However, traditional industrial hydrogen production methods, such as methane cracking, water gas conversion, water electrolysis, etc., are costly, consume a lot of resources and energy, and cause serious environmental pollution. [0003] Fermentative biohydrogen production technology u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P3/00C12N1/20C12R1/01
CPCC12P39/00C12P3/00C12N1/20Y02E60/36
Inventor 邢德峰李真
Owner HARBIN INST OF TECH
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