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Combined pretreatment method for synergically degrading straw cellulose through compound florae and producing hydrogen through fermentation

A technology of straw cellulose and composite flora, which is applied in the direction of fermentation, can solve the problems of resource waste and environmental pollution, and achieve the effect of improving hydrogen production rate and straw degradation rate

Inactive Publication Date: 2016-11-16
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the lack of effective utilization of it at present, it has caused a huge waste of resources, and the usual treatment method of incineration is also likely to cause serious environmental pollution.

Method used

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  • Combined pretreatment method for synergically degrading straw cellulose through compound florae and producing hydrogen through fermentation
  • Combined pretreatment method for synergically degrading straw cellulose through compound florae and producing hydrogen through fermentation
  • Combined pretreatment method for synergically degrading straw cellulose through compound florae and producing hydrogen through fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Combined pretreatment method of compound bacterial flora synergistically degrading straw cellulose and fermenting hydrogen production

[0027] Methods as below:

[0028] 1) Corn stalk crushing

[0029] Corn stalks were obtained from the suburbs of Shenyang City. The corn stalks are cut into small sections of 3-5 cm, and then these small sections are pulverized into powders of 30-60 meshes by using a miniature plant pulverizer to obtain straw powder.

[0030] 2) Combined pretreatment of corn stalks

[0031] According to the solid-to-liquid ratio of 1:10 (g / ml), straw powder and 1.0% NaOH solution were taken, soaked at room temperature for 24 hours, washed with water until neutral, and dried at 80°C.

[0032] According to the solid-liquid ratio of 1:10 (g / ml), take the straw powder pretreated by NaOH solution and the sulfuric acid aqueous solution with a concentration of 1% by volume, put them in a container, and mix them evenly. Seal the container, put it i...

Embodiment 2

[0036] The comparative experiment of embodiment 2 pretreatment method

[0037] The method steps are the same as in Example 1, except that the pretreatment method in step 2) is changed. use as figure 2 In the device shown, different serum bottles 1 use different pretreated straw substrates. Specifically:

[0038] A: In Example 1, combined pretreatment of corn stalk powder (ESCS) with sodium hydroxide and acidified steam explosion combined pretreatment.

[0039] B: Sulfuric acid pretreatment: respectively prepare H with a volume percentage concentration of 0.2%, 0.5%, and 1.0%. 2 SO 4 solution with a solid-to-liquid ratio of 1:10, soaked at room temperature for 24 hours; the treated corn stalks were washed with water until neutral, and then dried at 80°C to obtain pretreated corn stalk powder that was pretreated with sulfuric acid alone.

[0040] C: Sodium hydroxide pretreatment: prepare NaOH solutions with a mass percent concentration of 0.5%, 1.0%, and 2.0%, respectively...

Embodiment 3

[0057] The screening of embodiment 3 compound bacterial strains

[0058] The strains used were taken from the activated sludge in the laboratory continuous flow stirred tank anaerobic fermentation hydrogen production reactor CSTR (ZL92114474.1). After separation and purification, 125 strains of hydrogen-producing bacteria were obtained, and the hydrogen-producing ability and fermentation end products of seven strains of hydrogen-producing bacteria B2, B19, H7, X9, X12, C3 and C17 with strong hydrogen-producing ability were analyzed by gas chromatography. According to optical microscope observation and gas chromatography analysis of end products, the isolated hydrogen-producing bacteria are divided into two types, the first type is ethanol-type fermentation hydrogen-producing strains B2, B19 and H7, etc.; the second type is butyric acid-type fermentation hydrogen production Bacteria X9, X12, C3 and C17 etc. The above seven hydrogen-producing bacteria were screened for the comp...

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Abstract

The invention relates to a combined pretreatment method for synergically degrading straw cellulose through compound florae and producing hydrogen through fermentation, belonging to the technical field of processing of solid waste. The combined pretreatment method for combining the compound florae with the combined pretreatment of sodium hydroxide and acidification steam explosion is utilized for carrying out efficient anaerobic fermentation biological hydrogen production for the first time. The method comprises the steps of firstly grinding straws into 30-60-mesh powder, carrying out sodium hydroxide pretreatment, finally carrying out acidification steam explosion pretreatment to obtain pretreated straw powder, adding the compound florae, culturing the mixture in an intermittent fermentation hydrogen production testing device, and carrying out the efficient anaerobic fermentation biological hydrogen production. According to the combined pretreatment method, by virtue of the combined pretreatment of sodium hydroxide and acidification steam explosion, the hydrogen production rate and the cellulose degradation rate of the straw fermentation hydrogen production are increased, and the recycling and the energy regeneration of lots of straws are realized.

Description

technical field [0001] The invention belongs to the technical field of solid waste treatment, and in particular relates to a method for combined pretreatment of waste stalks, then complex bacterial flora synergistically degrading cellulose and biological hydrogen production under anaerobic fermentation conditions. Background technique [0002] Straw is a kind of lignocellulosic raw material with wide distribution and huge reserves, and it is also an abundant and cheap renewable resource. However, due to the lack of effective use of it at present, it has caused a huge waste of resources, and the usual treatment method of incineration is also likely to cause serious environmental pollution. Using the bioconversion of lignocellulosic raw materials represented by corn stalks to produce clean, efficient and safe new energy-hydrogen has gradually become a research hotspot and has attracted great attention at home and abroad. It has very far-reaching economic value and strategic s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00
CPCC12P3/00C12P2201/00
Inventor 包红旭杨华张浩刘一晨苏弘治王爱杰任南琪史英君李丽丹沈曼莉
Owner LIAONING UNIVERSITY
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