This invention involves the technology of extraction of beta-mannan enzyme from Erwinia bacilli in the industry of biological agents. The technical program includes original species activation, primary seed cultivation, secondary seed cultivation, fermentation, proteinase inhibitor, crude separation, refining of concentrated efficient bacilli and mannan-liquid, formulas, vacuum packaging technology and condensed efficient liquid agent and mannan enzyme products. This invention uses centralized preparation agent program to reduce technical difficulty of bio-degumming and biological pulping process, and can reduce the production cost by extracting cell pellets and secretions from the same broth. The efficient concentrated liquid agent, produced by this invention technical programs, is suitable for bio-degumming of crudefiber plants, biological pulping of grasses and biological glycosylated processing of herbaceous fiber raw materials biopulping alcohol; mannan enzymes are widely used in food, medicine, fodder, paper, textiles, petroleum mining industry.