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Method for producing hydrogen through kitchen waste enzymolysis and reinforced dark fermentation

The technology of kitchen waste and dark fermentation is applied in the field of hydrogen production through enzymatic hydrolysis of kitchen waste to strengthen dark fermentation, which can solve the problems of high cost, complicated process and high energy consumption, and achieves low cost, simple process and improved hydrogen production rate. Effect

Inactive Publication Date: 2012-02-29
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese invention patent with the application number 200710029440.x discloses a method for two-phase anaerobic fermentation of food waste to produce hydrogen and methane. Under atm conditions, heat treatment for 0.25-1.0h, two-phase anaerobic fermentation to produce hydrogen and methane, has the problems of complex process, high energy consumption and high cost

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  • Method for producing hydrogen through kitchen waste enzymolysis and reinforced dark fermentation
  • Method for producing hydrogen through kitchen waste enzymolysis and reinforced dark fermentation

Examples

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Embodiment 1

[0027]Fill the bucket with dewatered sludge from the sewage plant to remove impurities such as silt and grass, cover the bucket and leave it for 15-20 days to create an anaerobic environment, remove the aerobic bacteria in it, and then take 100g from the middle and lower part of the bucket Put the dehydrated sludge into a fermentation bottle, seal it, and heat it at 80°C for 0.5h, then add 1.2g of glucose and 1g of peptone, shake well, and seal it. The fermentation bottle is acclimated at 50-53°C, and the sludge is acclimated until no gas is produced, and the time is 20-30 hours. The acclimatized sludge is the inoculation sludge. Heat and gelatinize 100g of food waste, add α-amylase (40u / 100g of food waste) to react for 4 hours at 80°C, then cool down to 55-60°C and then add glucoamylase (40u / 100g of food waste) for heating reaction 4h, cool down. After saccharification, add food waste and inoculated sludge into the fermentation bottle and shake well, add water to adjust the ...

Embodiment 2

[0029] Take 100g of the sludge left in the anaerobic state of Example 1 for 10-20 days, put it into a fermentation bottle, seal it, heat treat it at 85°C for 0.5h, then add 2g of glucose and 0.9g of peptone, shake it up, and seal it. The fermentation bottle is acclimatized at 50-53°C, and the sludge is acclimated until no gas is produced, and the time is 20-30 hours. The acclimatized sludge is recorded as inoculation sludge. Heat and gelatinize 100g of food waste, add α-amylase (20u / 100g of food waste) to react for 2 hours at 80°C, then cool down to 55-60°C and add glucoamylase (20u / 100g of food waste) for heating reaction 2h, cooling. After saccharification, add food waste and inoculated sludge into the fermentation bottle and shake well, add water to adjust the solid content between 5-10wt% (preferably 6-8%), seal it, control the dark fermentation reaction temperature at 50-53°C, hydrogen The yield is 73.07~94.36mLH 2 / gVS, after 14-20 hours of fermentation time, the proce...

Embodiment 3

[0031] Take 100g of the sludge left in the anaerobic state of Example 1 for 10-20 days, put it into a fermentation bottle, seal it, and heat-treat it at 83°C for 0.5h, then add 0.9g of glucose and 1.2g of peptone, shake well, and seal it. The fermentation bottle was acclimated at 53°C, and the sludge was acclimated until no gas was produced, and the time was 20-30 hours. The acclimated sludge was recorded as inoculation sludge. Heat and gelatinize 100g of food waste, add α-amylase (40u / 100g of food waste) to react for 6 hours at 80°C, then cool down to 55-60°C and add glucoamylase (40u / 100g of food waste) for heating reaction 6h, cooling. After saccharification, add food waste and inoculated sludge into the fermentation bottle and shake well, add water to adjust the solid content between 5-10wt% (preferably 6-8%), seal it, control the dark fermentation reaction temperature at 50-53°C, hydrogen The yield is 98.18~107.66mLH 2 / gVS, after 12-18 hours of fermentation time, the p...

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Abstract

The invention discloses a method for producing hydrogen through kitchen waste enzymolysis and reinforced dark fermentation. The method comprises steps that: impurities are removed from dehydrated sludge discharged from a sewage factory; the sludge is settled for 10 to 20 days under anoxic stress, and is sealed and processed through heat treatment; glucose and peptone are added to the sludge, and the sludge is cultivated and acclimatized under a temperature of 50 to 53 DEG C, such that inoculated sludge is obtained; alpha-amylase and glucoamylase are added to kitchen waste for carrying out saccharification; the saccharified kitchen waste and the inoculated sludge are added into a fermentation bottle, and anaerobic fermentation is carried out under a temperature of 50 to 53 DEG C, such thathydrogen is produced; when the gas production is almost stopped, the reaction is stopped. According to the invention, alpha-amylase and glucoamylase are used for carrying out pre-treatment upon kitchen waste for a short time, such that a fermentation substrate micromolecular glycan which is rich and easy to utilize is provided for hydrogenogens. Therefore, fermentation time is shortened, and hydrogen yield is improved. The method provided by the invention has advantages of simple technology, fast starting, and low cost.

Description

technical field [0001] The invention relates to a method for producing hydrogen, in particular to a method for producing hydrogen by enzymatically decomposing food waste and strengthening dark fermentation. Background technique [0002] Kitchen waste, also known as slops, is a general term for leftovers and kitchen leftovers discarded by families and catering units, and is also an important part of urban domestic waste. With the development of society and the continuous improvement of people's living standards, the output of kitchen waste is also increasing year by year. According to relevant statistics, in 2007, the annual output of food waste in the whole country was about 90 million tons. Taking Beijing, Shanghai, Guangzhou and Hangzhou as examples, the daily output of food waste exceeded 1000 tons. Food waste has high moisture content, complex composition, perishable rancidity, and is prone to mosquitoes and flies. However, the current treatment methods in foreign count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12P19/14
Inventor 张记市
Owner QILU UNIV OF TECH
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