Method for producing hydrogen through biomass fermentation and photosynthetic coupling

Abstract

The invention belongs to the field of biotechnology, and in particular relates to a method for producing hydrogen by utilizing sweet potato, yam and other biomass fermentation and photosynthetic coupling. The method completes fermentative hydrogen production and photosynthetic hydrogen production in the same reactor, and comprises that: biomass is subjected to heat treatment first; a fermentation medium taking the biomass as a substrate is inoculated with fermentative bacteria; anaerobic fermentative hydrogen production is performed in an environment at the temperature of between 25 and 40 DEG C with a pH between 5.5 and 7.5; and after hydrogen production is completed, fermentation broth does not need to be added with any other nutrient substances or be diluted, and only needs to have pH regulated to be between 6.0 and 7.5 and then be directly inoculated with hybrid photosynthetic flora so as to continue anaerobic photosynthetic hydrogen production with the light intensity between 2,000 and 8, 000 lux. In the method, final fermentation broth after hydrogen production only needs to be added with sweet potatoes, yams and other biomass again and then can be used for coupling hydrogen production again, and the fermentation broth can be reused for 3 to 5 times. The method has the advantages of high hydrogen production efficiency, long duration of hydrogen production, high substrate conversion rate, easiness for industrialization, and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for utilizing sweet potato, yam and other biomass fermentation-photocoupling joint production of hydrogen. Background technique [0002] As a clean and environmentally friendly hydrogen production method, biological hydrogen production has attracted widespread attention. There are two main methods for biological hydrogen production: fermentation hydrogen production and photosynthetic hydrogen production. Fermentative hydrogen production mainly uses strict anaerobic bacteria and facultative anaerobic bacteria to decompose some macromolecular organic matter (such as starch, cellulose, etc.) to produce hydrogen. The hydrogen production rate is fast and the substrate conversion rate is high. A large amount of volatile organic acids (acetic acid, butyric acid, and propionic acid) produced will cause a drop in pH, thereby affecting the efficiency of hydrogen production...

AI Technical Summary

Problems solved by technology

[0008] Sweet potato is one of the main food crops in my country. At present, the application of sweet potato in my country is limited to extracting its starch. There have been some reports on the use of sweet potato to generate ethanol, but there is no report on the use of sweet potato to generate hydrogen.

[0009] Dioscorea Zingiberensis C.H Wright is a perennial twisting herb of the Dioscorea family Dioscorea genus, commonly known as turmeric, fire head root, and is the main plant for extracting diosgenin, a raw material for steroid hormone drugs. Solution: While decomposing diosgenin, the starch and cellulose in Dioscorea scutellariae enter the wastewater without being utilized, which not only wastes a lot of resources, but also makes wastewater treatment more difficult and seriously pollutes the environment