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Method for aerobically producing hydrogen by respiration interaction of aerobic hydrogen producing bacteria and aerobic bacteria

一种氢气、菌粉的技术，应用在基于微生物的方法、生物化学设备和方法、细菌等方向，能够解决不利生物制氢扩大化、条件苛刻、操作繁琐等问题，达到减轻底物竞争、提高产率、纯度高的效果

Active Publication Date: 2017-09-01
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the need in the prior art to continuously remove the oxygen in the medium by boiling the medium and then injecting nitrogen to blow off the air, this method is cumbersome to operate and the conditions are harsh, which is not conducive to the expansion of biological hydrogen production. Boiling and exposure It is also easy to cause the loss of reagents in the process of aeration, and then produce problems such as hydrogen impurity. The present invention aims to provide a new way for the cultivation of hydrogen-producing bacteria, and to provide an aerobic respiration interaction between anaerobic hydrogen-producing bacteria and aerobic bacteria. The hydrogen production method specifically relates to a method for producing hydrogen by co-cultivating facultative bacteria and strictly anaerobic hydrogen-producing bacteria using a culture medium without anaerobic treatment. The technical scheme adopted is as follows:

Method used

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  • Method for aerobically producing hydrogen by respiration interaction of aerobic hydrogen producing bacteria and aerobic bacteria
  • Method for aerobically producing hydrogen by respiration interaction of aerobic hydrogen producing bacteria and aerobic bacteria
  • Method for aerobically producing hydrogen by respiration interaction of aerobic hydrogen producing bacteria and aerobic bacteria

Examples

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Effect test

Embodiment 1

[0032] Example 1: Co-culture hydrogen production with sucrose as carbon source

[0033] This embodiment provides a method for aerobic hydrogen production using the respiration interaction between Ethanoligenens harbinense YUAN-3 and Pseudomonas aeruginosa PAO1, which is implemented in the following steps:

[0034]1. Cultivate Harbin Ethanologenic Bacillus YUAN-3 by anaerobic culture method: Culture Harbin Ethanologenic Bacillus YUAN-3 with liquid medium A after high-temperature steam sterilization, and place the inoculated anaerobic bottle in a constant temperature shaker Cultivate with shaking at 35°C and 170rpm. After 40 hours, agglutinate and form white spherical bacterial micelles at the bottom of the culture bottle. Keep the supernatant clear, centrifuge at 8000 rpm for 5 minutes, and discard the supernatant;

[0035] 2. Cultivate Pseudomonas aeruginosa PAO1 by aerobic culture method: use liquid medium B sterilized by high temperature steam to cultivate Pseudomonas aerugi...

Embodiment 2

[0049] Example 2: Co-culture hydrogen production with lactose as carbon source

[0050] This embodiment provides a method for aerobic hydrogen production using the respiration interaction between Ethanoligenens harbinense YUAN-3 and Pseudomonas aeruginosa PAO1, which is implemented in the following steps:

[0051] 1. Cultivate Harbin Ethanologenic Bacillus YUAN-3 by anaerobic culture method: Culture Harbin Ethanologenic Bacillus YUAN-3 with liquid medium A after high-temperature steam sterilization, and place the inoculated anaerobic bottle in a constant temperature shaker Cultivate with shaking at 35°C and 170rpm. After 40 hours, agglutinate and form white pellets at the bottom of the culture bottle. The supernatant remains clear. Centrifuge at 8000 rpm for 5 minutes and discard the supernatant;

[0052] 2. Cultivate Pseudomonas aeruginosa PAO1 by aerobic culture method: use liquid medium B sterilized by high temperature steam to cultivate Pseudomonas aeruginosa, and place th...

Embodiment 3

[0066] Example 3: Hydrogen production by fermentation with glucose as substrate

[0067] This embodiment provides a method for aerobic hydrogen production using the respiration interaction between Ethanoligenens harbinense YUAN-3 and Pseudomonas aeruginosa PAO1, which is implemented in the following steps:

[0068] 1. Cultivate Harbin Ethanologenic Bacillus YUAN-3 by anaerobic culture method: Culture Harbin Ethanologenic Bacillus YUAN-3 with liquid medium A after high-temperature steam sterilization, and place the inoculated anaerobic bottle in a constant temperature shaker Cultivate with shaking at 35°C and 170rpm. After 40 hours, agglutinate and form white pellets at the bottom of the culture bottle. The supernatant remains clear. Centrifuge at 8000 rpm for 5 minutes and discard the supernatant;

[0069] 2. Cultivate Pseudomonas aeruginosa PAO1 by aerobic culture method: use liquid medium B sterilized by high temperature steam to cultivate Pseudomonas aeruginosa, and place t...

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Abstract

The invention discloses a method for aerobically producing hydrogen by respiration interaction of aerobic hydrogen producing bacteria and aerobic bacteria, and belongs to the technical field of hydrogen production by fermentation. The method comprises co-culturing Ethanoligenens harbinense YUAN-3 and Pseudomonas aeruginosa PAO1, specifically, inoculating Ethanoligenens harbinense YUAN-3 powder and Pseudomonas aeruginosa PAO1 powder into a sterilized liquid medium, performing sealed culture in a 35 DEG C constant temperature culture room for 1-50 h, and collecting the produced hydrogen. The invention provides a new route for culturing hydrogen producing bacteria, and the hydrogen gas prepared by the method of the invention has higher purity and yield. The method of the invention is simple in operation, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to an aerobic hydrogen production method through respiration interaction between anaerobic hydrogen-producing bacteria and aerobic bacteria, and belongs to the technical field of fermentation hydrogen production. Background technique [0002] Traditional anaerobic culture methods mainly include chemical methods (using chemical substances with strong reducing effect to absorb oxygen in the environment or culture medium, or reducing oxidation-reduction potentials with reducing and oxidizing substances), physical methods (using heating, Physical methods such as sealing and pumping to drive out or isolate the environment and the oxygen in the culture medium) and biological methods (the culture medium contains plant tissues to consume oxygen, or the anaerobic bacteria and aerobic bacteria are cultivated in the same dish) superior). [0003] Hydrogen production by biological fermentation is a stable, cost-effective method for producing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P3/00C12R1/385C12R1/01
CPCC12P3/00C12P39/00C07H3/02C07H3/04C12N1/20C07C323/58C12N1/205C12R2001/145C12R2001/385
Inventor 邢德峰于娜娇娃李真杨洋李威
Owner HARBIN INST OF TECH
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