Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide
A liquid bacterial agent and microbial technology, which is applied in the field of microbial liquid bacterial agent containing Pantoea camelii and its preparation, can solve the problems of easy inactivation and low content of effective bacteria, so as to promote rapid proliferation, improve nutrient absorption, The effect of extending shelf life
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[0046] Example 1 Fermentation of Promoting Bacteria
[0047] Prepare the microbial agent of the present invention as follows:
[0048] The Pantoea alhagi CGMCC NO.15525 stored on a slope at 4°C was inoculated on the eggplant-shaped culture flask medium (i.e. activation medium), and cultured at 30°C for 16 hours. Pick the strains on the activation medium, inoculate it in the fermentation medium, cultivate at 30°C for 40 hours, place the fermentation broth or fermentation broth concentrate in a cold storage at 4°C for 48 hours, and measure by plate coating. The number of viable bacteria in the fermentation broth can reach 1×10 10 Above CFU / mL. The medium in the eggplant-shaped culture flask is LB medium; the fermentation medium components are: sucrose 50g / L, beef extract 3g / L, NaCl 8g / L, peptone 8g / L, and pH 6.0-8.0.
[0049] Activate the Bacillus subtilis CCTCC NO:M 2016264, Bacillus jelly-like CCTCCNO:M2016265, and Bacillus amyloliquefaciens CCTCC NO:M 2016266 stored on a slant at ...
Example Embodiment
[0052] Example 2 Preparation of composite microbial agent
[0053] The composite microbial liquid inoculum containing the combination of polyglutamic acid, biological organic matter, and growth-promoting bacteria of the present invention is prepared according to the following method:
[0054] 1) Polyglutamic acid is prepared by fermentation (reference Xu Z, Feng X, Dan Z, et al. Enhanced poly(γ-glutamic acid) fermentation by Bacillus subtilis, NX-2immobilized in anaerobic plant fibrous-bed bioreactor[J] .Bioresour Technol,2014,155:8-14), the polyglutamic acid content of the fermentation broth after concentration is 15%;
[0055] 2) Molasses are purchased from sugar factories;
[0056] 3) Mix the composite growth-promoting bacteria liquid of Example 1 with the polyglutamic acid fermentation concentrate and molasses at a mass ratio of 1:2.5:6.5 to prepare the composite microbial agent of the present invention. The detection showed that the mass percentage of polyglutamic acid was 4.0%,...
Example Embodiment
[0057] Example 3 The composite microbial liquid inocula can be stored open for up to 24 months
[0058] The bacterial agents used in this embodiment are as follows:
[0059] 1) Dilute the mixed bacterial agent prepared in Example 1 to an effective viable number of 7.6×10 9 cfu / mL, as bacterial agent 1;
[0060] 2) Mix the mixed bacterial agent in Example 1 with the polyglutamic acid fermentation concentrate in proportion to make the polyglutamic acid content 4.0% and the effective viable bacteria number 7.6×10 9 cfu / mL, as bacterial agent 2;
[0061] 3) Mix the mixed bacterial agent and molasses in Example 1 uniformly in proportion, so that the organic matter content is 63%, and the effective number of viable bacteria is 7.6×10 9 cfu / mL, as bacterial agent 3;
[0062] 4) Mix the mixed bacterial agent prepared in Example 1 with polyglutamic acid and molasses uniformly in proportions so that the polyglutamic acid content is 4.0%, the organic matter content is 15%, and the effective number...
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