Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide

A liquid bacterial agent and microbial technology, which is applied in the field of microbial liquid bacterial agent containing Pantoea camelii and its preparation, can solve the problems of easy inactivation and low content of effective bacteria, so as to promote rapid proliferation, improve nutrient absorption, The effect of extending shelf life

Active Publication Date: 2018-11-27
NANJING SHINEKING BIOTECH CO LTD
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In order to solve the problem of low effective bacteria content and easy inactivation of existing microbial liquid inoculum, the present invention provides a microbial liquid inoculum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide
  • Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide
  • Microbial liquid fungicide containing pantoea alhagi and preparation method and application of microbial liquid fungicide

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1 Fermentation of Promoting Bacteria

[0047] Prepare the microbial agent of the present invention as follows:

[0048] The Pantoea alhagi CGMCC NO.15525 stored on a slope at 4°C was inoculated on the eggplant-shaped culture flask medium (i.e. activation medium), and cultured at 30°C for 16 hours. Pick the strains on the activation medium, inoculate it in the fermentation medium, cultivate at 30°C for 40 hours, place the fermentation broth or fermentation broth concentrate in a cold storage at 4°C for 48 hours, and measure by plate coating. The number of viable bacteria in the fermentation broth can reach 1×10 10 Above CFU / mL. The medium in the eggplant-shaped culture flask is LB medium; the fermentation medium components are: sucrose 50g / L, beef extract 3g / L, NaCl 8g / L, peptone 8g / L, and pH 6.0-8.0.

[0049] Activate the Bacillus subtilis CCTCC NO:M 2016264, Bacillus jelly-like CCTCCNO:M2016265, and Bacillus amyloliquefaciens CCTCC NO:M 2016266 stored on a slant at ...

Example Embodiment

[0052] Example 2 Preparation of composite microbial agent

[0053] The composite microbial liquid inoculum containing the combination of polyglutamic acid, biological organic matter, and growth-promoting bacteria of the present invention is prepared according to the following method:

[0054] 1) Polyglutamic acid is prepared by fermentation (reference Xu Z, Feng X, Dan Z, et al. Enhanced poly(γ-glutamic acid) fermentation by Bacillus subtilis, NX-2immobilized in anaerobic plant fibrous-bed bioreactor[J] .Bioresour Technol,2014,155:8-14), the polyglutamic acid content of the fermentation broth after concentration is 15%;

[0055] 2) Molasses are purchased from sugar factories;

[0056] 3) Mix the composite growth-promoting bacteria liquid of Example 1 with the polyglutamic acid fermentation concentrate and molasses at a mass ratio of 1:2.5:6.5 to prepare the composite microbial agent of the present invention. The detection showed that the mass percentage of polyglutamic acid was 4.0%,...

Example Embodiment

[0057] Example 3 The composite microbial liquid inocula can be stored open for up to 24 months

[0058] The bacterial agents used in this embodiment are as follows:

[0059] 1) Dilute the mixed bacterial agent prepared in Example 1 to an effective viable number of 7.6×10 9 cfu / mL, as bacterial agent 1;

[0060] 2) Mix the mixed bacterial agent in Example 1 with the polyglutamic acid fermentation concentrate in proportion to make the polyglutamic acid content 4.0% and the effective viable bacteria number 7.6×10 9 cfu / mL, as bacterial agent 2;

[0061] 3) Mix the mixed bacterial agent and molasses in Example 1 uniformly in proportion, so that the organic matter content is 63%, and the effective number of viable bacteria is 7.6×10 9 cfu / mL, as bacterial agent 3;

[0062] 4) Mix the mixed bacterial agent prepared in Example 1 with polyglutamic acid and molasses uniformly in proportions so that the polyglutamic acid content is 4.0%, the organic matter content is 15%, and the effective number...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a microbial liquid fungicide containing pantoea alhagi. The microbial liquid fungicide containing the pantoea alhagi comprises the following components in percentages by mass:1-10% of promoting growth bacteria fluid, 0.1-10% of polyglutamic acid, 50-70% of biological organic matters and the balance of water or aqueous solution. The promoting growth bacteria fluid comprisespantoea alhagi fluid. The invention further discloses a preparation method and application of the composite microbial liquid fungicide. The composite microbial liquid fungicide has a high-activity biological function, wherein the polyglutamic acid, the biological organic matters and a microbial agent have a synergistic effect, a microbiome environment of root systems of crops can be improved, thenumber of beneficial microorganisms in soil is increased, the quality of the crops can be improved, the yield is increased, and resistance against disadvantages such as drought resistance, salt resistance, cold resistance and disease resistance of the crops is enhanced.

Description

technical field [0001] The invention relates to a microbial inoculum, in particular to a microbial liquid inoculum containing Pantoea camelis and a preparation method and application thereof. Background technique [0002] my country is a big agricultural country in the world. With the rapid development of my country's agriculture, the use of chemical fertilizers is also increasing, reaching 1.8 million tons per year. Although this solved the problem of increasing food production and supply, it caused very serious environmental problems. The excessive use of chemical fertilizers has aggravated soil compaction and pollution, leading to the decline and degradation of soil quality, which makes the content of soil organic matter seriously lower than the global average level. Once the soil organic matter content is low, it will greatly reduce the ability to fix heavy metals, increase the harmfulness of soil heavy metals, and endanger the sustainable development of agriculture in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 冯小海徐虹许宗奇周哲
Owner NANJING SHINEKING BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products