Saline-alkali soil composite conditioner and application method thereof

A saline-alkali land and soil improver technology, applied in the field of soil restoration, can solve the problems of inability to convert saline-alkali land, little application value and promotion significance, and reduce the salt content of saline-alkali land, so as to improve the level of plant growth hormone, strengthen the improvement effect, increase The effect of soluble phosphorus

Active Publication Date: 2019-06-07
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the soil salinity is too high, the growth of salt-tolerant plants and halophytes will also be inhibited; and this method can only reduce the salt content of saline-

Method used

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  • Saline-alkali soil composite conditioner and application method thereof
  • Saline-alkali soil composite conditioner and application method thereof
  • Saline-alkali soil composite conditioner and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1: Screening and identifying required microorganisms

[0047] 1. The medium and reagents involved in this embodiment are as follows:

[0048] (1) Beef extract peptone liquid culture medium: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, adjust pH=7.0, and sterilize at 121°C for 20min. Add 18 g of agar to the beef extract peptone liquid medium to obtain a beef extract peptone solid medium.

[0049] (2) Ashby nitrogen-free liquid medium: mannitol 10g, CaCO 3 5g, KH 2 PO 3 0.2g, MgSO 4 ·7H 2 O0.2g, NaCl 0.2g, CaSO 4 ·2H 2 O 0.1g, pH=7.4, 1000mL deionized water.

[0050] (3) Phosphorus solution liquid medium: glucose 10g, calcium phosphate 5g, ammonium sulfate 0.1g, potassium chloride 0.2g, heptahydrate magnesium chloride 0.25g, distilled water 1000mL, pH 6.8-7.0, autoclaved at 115°C for 30 min.

[0051] (4) DF liquid medium: MnSO 4 ·7H 2 O 0.2g, KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, citric acid 2.0g, glucose 2.0g, sodium gluconate 2.0g, (NH 4 ) 2 S...

Example Embodiment

[0086] Example 2: Display of the salt-tolerant ability of the selected strains

[0087] 1. Preparation of seed liquid: the strains YZX4 and YX7 screened in Example 1 were respectively inoculated into beef extract peptone solid medium for activation: cultivated in a constant temperature incubator at 30°C for 8 hours. Pick a loop of activated functional strains YZX4 and YX7 into beef extract peptone liquid medium, and cultivate them to the logarithmic phase (determined by growth curve and OD value) at 28° C. and 180 r / min to obtain seed liquid.

[0088] 2. Salt tolerance test: Take an inoculated loop of YZX4 and YX7 bacteria (seed liquid) and inoculate them into beef extract peptone liquid containing 0, 20, 50, 70, 100, 120, 150, 200g / L NaCl. In the medium (pH=7.2), the non-inoculated beef extract peptone liquid medium was used as a blank control, and cultured on a shaker at 30°C and 180r / min for 2 days. Measure the absorbance value (OD 600 ).

[0089] Alkali tolerance test: Take an ...

Example Embodiment

[0091] Example 3: Screening of desalting agent in improver

[0092] 1. Prepare a 30g / L NaCl solution, measure its pH value and pNa value, the measurement result is that the pH value is 5.77 and the pNa value is 0.79. Take 50ml of the above solution and mix it with 1.6g of desalting agent uniformly, place it in a constant temperature shaker at 30°C and 160r / min, and measure the pH value and pNa value of each bottle solution after 24h. The desalting agents selected in this experiment are D001 type resin, AB500 type resin, D113 type resin and zeolite powder, with three replicates for each type of desalting agent.

[0093] 2. The measurement results are shown in Table 3.

[0094] Table 3 Display of determination results of different desalting agents

[0095] Desalting agent type

[0096] The results showed that the pH value of the NaCl solution after D001 treatment decreased from the initial 5.77 to 1.34 ± 0.02, and the pNa value increased from the initial 0.79 to 2.02 ± 0.07. The eff...

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Abstract

The invention belongs to the field of soil remediation, and particularly relates to a saline-alkali soil composite conditioner and an application method thereof. The invention particularly provides anovel bacillus subtilis, and a soil conditioner prepared by using the bacillus subtilis. The soil conditioner comprises the following specific components in parts by weight: 5-10 parts of a microbialagent, 70-80 parts of organic fertilizer, 10-20 parts of biochar and 5-10 parts of a desalting agent. The invention also provides a series of methods for improving saline-alkali soil based on the soilconditioner. The method provided by the invention is free from secondary pollution, and environmentally-friendly, and can thoroughly improve the severe saline-alkali soil into the soil which can be cultivated normally.

Description

technical field [0001] The invention belongs to the field of soil restoration, and in particular relates to a compound improver for saline-alkali land and an application method thereof. Background technique [0002] The accumulation of salinity in soil is the result of the superposition of a series of natural and human factors acting on different temporal and spatial scales. Soil salinization seriously restricts agricultural development, improving and restoring large areas of saline-alkali wasteland resources is of great significance to the development of agricultural production, land management, and ecological environment protection. [0003] At present, the improvement methods of saline-alkali land mainly include physical methods, chemical methods and biological methods. The physical method is mainly based on leaching and salt discharge, combined with plowing, leaching, silting and other measures to achieve the purpose of improving saline-alkaline soil; the chemical metho...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/40C09K109/00C09K101/00C12R1/125C12R1/38
Inventor 李东孙晓莹陈意超刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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