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Saline-alkali soil composite conditioner and application method thereof

A saline-alkali land and soil improver technology, applied in the field of soil restoration, can solve the problems of inability to convert saline-alkali land, little application value and promotion significance, and reduce the salt content of saline-alkali land, so as to improve the level of plant growth hormone, strengthen the improvement effect, increase The effect of soluble phosphorus

Active Publication Date: 2019-06-07
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the soil salinity is too high, the growth of salt-tolerant plants and halophytes will also be inhibited; and this method can only reduce the salt content of saline-alkali land to a certain extent, and cannot completely convert saline-alkali land into arable land, so , the practical application value and promotion significance are not great

Method used

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  • Saline-alkali soil composite conditioner and application method thereof
  • Saline-alkali soil composite conditioner and application method thereof
  • Saline-alkali soil composite conditioner and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Screening and identification of desired microorganisms

[0047] 1. The medium and reagents involved in this embodiment are as follows:

[0048] (1) Beef extract-peptone liquid medium: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1000mL, adjust pH=7.0, sterilize at 121°C for 20min. Add 18 g of agar to the beef extract peptone liquid medium to obtain the beef extract peptone solid medium.

[0049] (2) Ashby nitrogen-free liquid medium: mannitol 10g, CaCO 3 5g, KH 2 PO 3 0.2g, MgSO 4 ·7H 2 O0.2g, NaCl 0.2g, CaSO 4 2H 2 O 0.1g, pH=7.4, deionized water 1000mL.

[0050] (3) Phosphorus-dissolving liquid medium: glucose 10g, calcium phosphate 5g, ammonium sulfate 0.1g, potassium chloride 0.2g, magnesium chloride heptahydrate 0.25g, distilled water 1000mL, pH 6.8-7.0, autoclaved at 115°C for 30min.

[0051] (4) DF liquid medium: MnSO 4 ·7H 2 O 0.2g, KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, citric acid 2.0g, glucose 2.0g, sodium gluconat...

Embodiment 2

[0086] Example 2: Salt-alkali tolerance display of the strains screened

[0087] 1. Preparation of seed solution: Inoculate the strains YZX4 and YX7 screened in Example 1 into beef extract peptone solid medium for activation: culture in a constant temperature incubator at 30°C for 8 hours. Pick an inoculation loop of activated functional strains YZX4 and YX7 into beef extract peptone liquid medium, and cultivate them at 28°C and 180r / min until the logarithmic phase of the strains (determined by growth curve and OD value) to obtain seed liquid.

[0088] 2. Salt tolerance test: Take an inoculation loop of YZX4 and YX7 bacteria (seed liquid) and inoculate them into beef extract peptone liquid containing 0, 20, 50, 70, 100, 120, 150, 200g / L NaCl In the culture medium (pH=7.2), the beef extract peptone liquid culture medium without inoculation was used as a blank control, and cultured on a shaker at 30° C. at 180 r / min for 2 days. The absorbance value (OD) of the culture medium at...

Embodiment 3

[0091] Example 3: Screening of desalting agent in improver

[0092] 1. Prepare 30g / L NaCl solution, measure its pH value and pNa value, the measurement result shows that the pH value is 5.77, and the pNa value is 0.79. Take 50ml of the above solution, mix it with 1.6g of desalting agent evenly, place it in a constant temperature shaker at 30°C and 160r / min, measure the pH value and pNa value of each bottle of solution after 24h. The desalting agents selected in this experiment were D001 resin, AB500 resin, D113 resin and zeolite powder, and each desalting agent was repeated three times.

[0093] 2. The measurement results are shown in Table 3.

[0094] Table 3 shows the measurement results of different desalting agents

[0095] Type of desalination agent

[0096] The results show that the pH value of the NaCl solution after D001 treatment is reduced from initial 5.77 to 1.34 ± 0.02, and the pNa value is increased to 2.02 ± 0.07 from initial 0.79. desalination agen...

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Abstract

The invention belongs to the field of soil remediation, and particularly relates to a saline-alkali soil composite conditioner and an application method thereof. The invention particularly provides anovel bacillus subtilis, and a soil conditioner prepared by using the bacillus subtilis. The soil conditioner comprises the following specific components in parts by weight: 5-10 parts of a microbialagent, 70-80 parts of organic fertilizer, 10-20 parts of biochar and 5-10 parts of a desalting agent. The invention also provides a series of methods for improving saline-alkali soil based on the soilconditioner. The method provided by the invention is free from secondary pollution, and environmentally-friendly, and can thoroughly improve the severe saline-alkali soil into the soil which can be cultivated normally.

Description

technical field [0001] The invention belongs to the field of soil restoration, and in particular relates to a compound improver for saline-alkali land and an application method thereof. Background technique [0002] The accumulation of salinity in soil is the result of the superposition of a series of natural and human factors acting on different temporal and spatial scales. Soil salinization seriously restricts agricultural development, improving and restoring large areas of saline-alkali wasteland resources is of great significance to the development of agricultural production, land management, and ecological environment protection. [0003] At present, the improvement methods of saline-alkali land mainly include physical methods, chemical methods and biological methods. The physical method is mainly based on leaching and salt discharge, combined with plowing, leaching, silting and other measures to achieve the purpose of improving saline-alkaline soil; the chemical metho...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/40C09K109/00C09K101/00C12R1/125C12R1/38
Inventor 李东孙晓莹陈意超刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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