Peanut rhizosphere bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacillus, applied in the direction of application, bacteria, biocide, etc., to achieve the effect of improving the microbial community structure, increasing the number, increasing the variety and abundance

Active Publication Date: 2017-08-18
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this strain only involves the study of a single antibacterial function, and Bacillus amyloliquefaciens with multiple growth-promoting mechanisms and biological control functions, such as growth-promoting, siderophore production, plant resis

Method used

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  • Peanut rhizosphere bacillus amyloliquefaciens and application thereof
  • Peanut rhizosphere bacillus amyloliquefaciens and application thereof
  • Peanut rhizosphere bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1: Isolation and screening of strains

[0036] 1. Isolation and screening of strains:

[0037] (1) Isolation of strains

[0038] The strain was sieved from the peanut rhizosphere soil, and the soil samples were collected from the peanut plantation in the suburbs of Tai'an City, Shandong Province. Carefully shake off the peanut rhizosphere soil into a sterile bag, put it in a low-temperature storage box, and take it back to the laboratory for immediate disposal. Weigh 10 g of rhizosphere soil sample, put it into 90 mL of sterile water, and shake for 30 minutes to make a microbial suspension. Draw 1mL of the suspension separately, and dilute it in a 10-fold ratio, then take 10 -4 , 10 -5 And 10 -6 Spread 200 μL of the doubling dilution on a beef extract peptone plate, let it stand for 10 minutes, and place it upside down in a 37°C constant temperature incubator for about 24 hours. Select representative colonies on the plate and purify by the three-zone streaking metho...

Example Embodiment

[0056] Example 2: Preparation of strain Y14 biocontrol preparation

[0057] The specific preparation method is as follows:

[0058] The preparation method of Y14 fermentation broth: transfer the Bacillus amyloliquefaciens Y14 stored on the slant into a test tube containing 10 mL of liquid LB medium, and place it in a constant temperature shaker for 12 hours (temperature: 37°C; rotation speed: 180 rpm). The activated bacteria solution was connected to 200mL liquid LB medium at 1% (volume percentage) inoculum, and cultured in a shaker for 24h (temperature: 37°C; rotation speed: 180rpm) to obtain the fermentation broth of strain Y14; When using biocontrol preparations, dilute the fermentation broth with a proper amount of sterile water to 1×10 8 cfu / ml is fine.

Example Embodiment

[0059] Example 3: Strain Y14 inhibits the growth of the pathogen of Sclerotia spp

[0060] 1. Panel confrontation test

[0061] (1) Materials and methods:

[0062] The plate confrontation test was used to inoculate the activated peanut S. elegans mycelium block cut with a sterile punch in the center of the PDA plate, culture for 1 day at 28°C, and inoculate the antagonistic antibacterial Y14 at about 3 cm from the edge of the sclerotium sp Only inoculate pathogenic bacteria as a control, and observe the antibacterial effect after culturing for 3 days. At the same time, pick out the sclerotium sclerotium mycelium on the opposite edge, and observe the growth and morphological changes of the sclerotium sclerotium under a microscope with the sclerotium spp.

[0063] (2) Test results:

[0064] Through plate confrontation culture, it was found that the strain Y14 had a significant antagonistic effect on the pathogen of peanut sclerotium ( figure 2 ), the control plate (A) mycelium grew vig...

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Abstract

The invention discloses bacillus amyloliquefaciens. The bacillus amyloliquefaciens has the classification and name of Bacillus amyloliquefaciens Y14 and is preserved in China General Microbiological Culture Collection Center (CGMCC for short) on November 10th, 2016 with the preservation address of Microbiology Research Institute of China Science Academe, No.3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing and the preservation number of CGMCC NO.13258. The strain has good inhibitory effect on pathogenic bacteria of southern blight and also can induce resistance of a botanical system, iron nutrition is provided for peanuts, a soil microbial community structure also can be improved, and a strong guarantee is provided for production of the peanuts.

Description

technical field [0001] The present invention relates to a strain of Bacillus amyloliquefaciens in peanut rhizosphere and its application, in particular to a strain of Bacillus amyloliquefaciens capable of preventing and treating peanut blight, and simultaneously having the function of promoting growth, improving peanut iron nutrition and rhizosphere microbial community structure, belonging to field of biotechnology. Background technique [0002] The pathogenic bacterium of peanut white silkworm is Selerotium rolfsii Sacc., which spreads by means of sclerotium, mycelium or infected plant debris, causing the roots of plants to rot and infecting a variety of plants. It is a worldwide epidemic soil-borne diseases. Peanut canker sores is a serious disease in my country, and occurs in all major peanut producing areas. Peanut canker sore can cause peanut stem rot and fruit rot, which seriously threatens the yield and quality of peanuts. At present, cultivation measures, breeding ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 丁延芹杜秉海姚良同刘凯汪城墙
Owner SHANDONG AGRICULTURAL UNIVERSITY
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