DDT (dichlorodiphenyltrichloroethane)-degrading pseudomonad strain

A technology of pseudomonas and microbial strains, applied in the field of pseudomonas, can solve the problems of environmental pollution, teratogenicity, high toxicity, etc., and achieve the effect of high application value

Inactive Publication Date: 2012-04-25
HARBIN NORMAL UNIVERSITY
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Problems solved by technology

Due to its teratogenic, carcinogenic and mutagenic "three-cause" effects, high toxicity and refractory characteristics, it has c

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  • DDT (dichlorodiphenyltrichloroethane)-degrading pseudomonad strain
  • DDT (dichlorodiphenyltrichloroethane)-degrading pseudomonad strain
  • DDT (dichlorodiphenyltrichloroethane)-degrading pseudomonad strain

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specific Embodiment approach 1

[0013] Specific embodiment 1: The Pseudomonas that degrades DDT in this embodiment is Pseudomonas sp. 12-3#, which is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city. The preservation date is November 08, 2011, and the preservation number is CGMCCNo.5449.

[0014] In this embodiment, Pseudomonas sp. 12-3# is a Gram-positive bacterium, an aerobic bacterium, rod-shaped, and the cell size is (0.1 μm to 0.2 μm)×(0.4 μm to 0.6 μm). Flagella, the staining picture of Pseudomonas 12-3# is as follows figure 1 As shown, the morphology of Pseudomonas 12-3# atomic mechanical microscope is as follows figure 2 As shown; when cultured on solid inorganic salt medium, the colony is round, light yellow in color, transparent, smooth in surface, neat in edge, viscous and easy to provoke. Wherein said solid inorgan...

specific Embodiment approach 2

[0017] Specific embodiment 2: In this embodiment, Pseudomonas sp. 12-3# is screened from DDT-contaminated soil in Guangzhou City. Screening was carried out according to the following steps: ① Take 10 mL of DDT-acclimated soil suspension and put it into a sterilized Erlenmeyer flask filled with glass beads and 90 mL of sterile water and shake it fully to break up the micelle groups and make the bacteria appear The single-cell state is dispersed in the liquid; ②Take 1mL of the bacterial suspension from the soil bacterial suspension in the Erlenmeyer flask, inoculate it in the solid inorganic salt medium with DDT as the only carbon source by the doubling dilution method, and place it upside down at 30 Cultivate in a constant temperature incubator at ℃ for 3 days; ③Pick out different typical single colonies, mark them on the plate, and culture them in a new culture plate by streaking, repeat the above operation 3 to 4 times, and obtain the pure 4. The obtained pure bacteria were t...

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Abstract

The invention relates to a DDT (dichlorodiphenyltrichloroethane)-degrading pseudomonad strain, and specifically provides a pseudomonad strain capable of degrading DDT, which can be used for the biological intensified treatment of DDT pollutants and can help to degrade DDT organochlorine pesticides. The DDT-degrading pseudomonad strain is named pseudomonad No.12-3; the strain was preserved by China General Microbiological Culture Collection Center on November 8th, 2011; and the preservation number is CGMCC NO.5449. The pseudomonad No.12-3 has the characteristic of degrading DDT organochlorine pesticides; the DDT degradation rate can be up to 51.6%; and secondary pollution can not be caused to the environment. Thus, the DDT-degrading pseudomonad strain can help to solve the problems of DDT degradation, pollution remediation and the like, thereby having high application value.

Description

technical field [0001] The present invention relates to a pseudomonas degrading DDT. Background technique [0002] DDT is also called DDT, and its chemical name is Dichlorodiphenyltrichloroethane (Dichlorodiphenyltrichloroethane). It is one of the 12 restricted POPs stipulated in the Stockholm Convention on Persistent Organic Pollutants (POPs). It is a heat-resistant, An organochlorine broad-spectrum insecticide with acid resistance, high fat solubility and long residual effect. Because of its "three causes" effects of teratogenicity, carcinogenicity and mutagenicity, high toxicity and refractory characteristics, it has caused serious pollution to the environment. Although it has been banned for decades, its cumulative effect in the environment is still serious. Therefore, it is imminent to use certain technical means to repair the environmental problems caused by the highly toxic, highly residual, and refractory DDT organochlorine pesticides. [0003] Microbial degradati...

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/38A62D101/04A62D101/22
Inventor 王继华刘翔牛佳辛佳宋北夏琳
Owner HARBIN NORMAL UNIVERSITY
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